protocol_161.ann

T1	Action 123 129	filter
E1	Action:T1 Acts-on:T14
T2	Action 136 146	centrifuge
E2	Action:T2 Acts-on:T14
T3	Action 192 196	spot
E3	Action:T3 Acts-on:T16 Site:T20
T4	Action 303 309	absorb
E4	Action:T4 Acts-on:T22
T5	Action 321 327	Remove
E5	Action:T5 Acts-on:T23
T6	Action 355 360	bring
E6	Action:T6 Acts-on:T25 Site:T26
T7	Action 475 481	remove
E7	Action:T7 Acts-on:T29 Using:T28
T8	Action 495 500	Stain
E8	Action:T8 Acts-on:T30 Using:E9
T9	Action 509 516	placing
E9	Action:T9 Acts-on2:T33 Site:T30
T10	Action 593 599	Remove
E10	Action:T10 Acts-on:T36 Using:T37
T11	Action 627 634	Air dry
E11	Action:T11 
T12	Action 672 679	examine
E12	Action:T12 Acts-on:T39 Using:T40
T13	Amount 83 86	5µL
T14	Reagent 93 111	enrichment culture
T15	Amount 116 122	0.2 µm
T16	Reagent 166 187	cell-free supernatant
T17	Time 149 155	10 min
T18	Speed 159 164	3000g
T19	Modifier 205 226	carbon/formvar-coated
T20	Location 227 252	electron microscope grids
T21	Time 314 319	2 min
T22	Mention 281 283	It
T23	Reagent 328 334	sample
T24	Size 366 371	small
T25	Device 383 404	piece of filter paper
T26	Location 438 454	side of the grid
T29	Reagent 460 466	sample
T28	Method 486 493	wicking
T30	Reagent 501 505	grid
T31	Amount 522 525	5μL
T32	Concentration 534 536	2%
T33	Reagent 537 551	Uranyl Acetate
T34	Reagent 556 572	Phosphotungstate
T35	Time 578 591	15-30 seconds
T36	Reagent 600 605	stain
T37	Method 609 616	wicking
T38	Time 648 658	10 minutes
T39	Reagent 660 667	Samples
T40	Method 684 716	transmission electron microscopy
R1	Measure Arg1:T14 Arg2:T13	
R2	Or Arg1:T13 Arg2:T15	
R3	Setting Arg1:E2 Arg2:T17	
R4	Setting Arg1:E2 Arg2:T18	
R5	Mod-Link Arg1:T20 Arg2:T19	
R6	Coreference-Link Arg1:T22 Arg2:T16	
R7	Setting Arg1:E4 Arg2:T21	
R8	Setting Arg1:T25 Arg2:T24	
T27	Modifier 405 430	perpendicular to the grid
R9	Mod-Link Arg1:T26 Arg2:T27	
R10	Measure Arg1:T33 Arg2:T32	
R11	Measure Arg1:T33 Arg2:T31	
R12	Meronym Arg1:T33 Arg2:T34	
R13	Setting Arg1:E9 Arg2:T35	
R14	Setting Arg1:E11 Arg2:T38