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protocol_165.ann
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T1 Action 72 79 Prepare
E1 Action:T1 Acts-on:T31 Using:T33
T2 Action 125 128 Add
E2 Action:T2 Acts-on:T35 Site:T36
T3 Action 195 201 vortex
E3 Action:T3 Acts-on:T36
T4 Action 278 284 vortex
E4 Action:T4 Acts-on:T40
T5 Action 230 238 transfer
E5 Action:T5 Using:T37 Acts-on:T39 Site:T40
T6 Action 203 209 Change
E6 Action:T6 Acts-on:T37
T7 Action 286 292 Repeat
E7 Action:T7 Using:T41 Acts-on:T42
T8 Action 319 323 Pour
E8 Action:T8 Acts-on:T43 Site:T44
T9 Action 387 391 fill
E9 Action:T9 Acts-on:T45
T10 Action 409 412 add
E10 Action:T10 Acts-on:T47 Using:T45 Site:T48
T11 Action 487 491 Fill
E11 Action:T11 Acts-on:T52
T12 Action 509 512 add
E12 Action:T12 Acts-on:T54 Using:T52 Site:T56
T13 Action 587 592 Empty
E13 Action:T13 Acts-on:T59
T14 Action 603 607 Pour
E14 Action:T14 Acts-on:T60 Site:T62
T15 Action 677 681 Fill
E15 Action:T15 Acts-on:T63
T16 Action 699 702 add
E16 Action:T16 Site:T67 Using:T63
T17 Action 737 742 Empty
E17 Action:T17 Acts-on:T68
T18 Action 753 759 Repeat
E18 Action:T18 Using:T69 Acts-on:T70
T19 Action 802 806 fill
E19 Action:T19 Acts-on:T71
T20 Action 855 861 Put on
E20 Action:T20 Acts-on:T74
T21 Action 874 878 seal
E21 Action:T21 Acts-on:T74 Using:T75
T22 Action 922 930 Incubate
E22 Action:T22
T23 Action 992 999 inspect
E23 Action:T23 Acts-on:T79 Using:T78
T24 Action 1065 1069 Mark
E24 Action:T24 Acts-on:T81
T25 Action 1117 1127 incubation
E25 Action:T25
T26 Action 1139 1150 inspections
E26 Action:T26
T27 Action 1179 1186 Prepare
E27 Action:T27 Acts-on:T82 Using:T83
T28 Action 1255 1264 Propagate
E28 Action:T28 Acts-on:T86 Site:T89
T29 Action 1327 1332 store
E29 Action:T29 Acts-on:T86
T30 Amount 80 81 9
T31 Location 82 87 tubes
T32 Amount 110 116 4.5 mL
T33 Reagent 117 123 medium
T34 Amount 129 135 500 μL
T35 Reagent 143 157 virus filtrate
T36 Location 183 190 tube #1
T37 Device 214 225 pipette tip
T38 Amount 239 245 500 μL
T39 Reagent 249 262 suspension #1
T40 Location 266 273 tube #2
T41 Method 297 306 procedure
T42 Location 310 317 tube #8
T43 Reagent 343 361 algal host culture
T44 Location 371 385 reservoir tray
T45 Device 396 404 pipetter
T46 Amount 413 419 150 μL
T47 Reagent 420 427 culture
T48 Location 436 440 well
T49 Modifier 431 435 each
T50 Location 444 453 lines 1–9
T51 Location 459 485 96-well cell culture plate
T52 Device 496 504 pipetter
T53 Amount 513 519 150 μL
T54 Reagent 520 527 culture
T55 Modifier 531 535 each
T56 Location 536 540 well
T57 Location 544 553 lines 1–9
T58 Location 559 585 96-well cell culture plate
T59 Location 597 601 tray
T60 Location 608 624 dilution tube #9
T61 Reagent 626 640 control medium
T62 Location 661 675 reservoir tray
T63 Device 686 694 pipetter
T64 Amount 703 709 100 μL
T65 Modifier 716 720 each
T66 Location 729 735 line 9
T67 Location 721 725 well
T68 Location 747 751 tray
T69 Method 760 773 steps 7 and 8
T70 Location 778 797 dilution tube #8–#1
T71 Location 822 832 well lines
T72 Modifier 811 821 respective
T73 Location 840 853 culture plate
T74 Seal 866 869 lid
T75 Seal 892 904 plastic tape
T76 Action 908 920 avoid drying
E30 Action:T76 Using:E21
T77 Modifier 879 886 tightly
T78 Device 968 987 inverted microscope
T79 Location 1004 1018 culture plates
T80 Time 1046 1063 regular intervals
T81 Location 1070 1075 wells
T82 Reagent 1196 1215 extinction dilution
T83 Reagent 1221 1226 virus
T84 Location 1249 1253 well
T85 Modifier 1236 1248 most-diluted
T86 Reagent 1265 1276 virus clone
T87 Modifier 1286 1298 most diluted
T88 Location 1299 1303 well
T89 Reagent 1309 1322 larger volume
R1 Measure Arg1:T31 Arg2:T30
R2 Measure Arg1:T33 Arg2:T32
R3 Measure Arg1:T35 Arg2:T34
R4 Measure Arg1:T39 Arg2:T38
R5 Measure Arg1:T47 Arg2:T46
R6 Mod-Link Arg1:T48 Arg2:T49
R7 Meronym Arg1:T48 Arg2:T50
R8 Meronym Arg1:T50 Arg2:T51
R9 Measure Arg1:T54 Arg2:T53
R10 Mod-Link Arg1:T56 Arg2:T55
R11 Meronym Arg1:T56 Arg2:T57
R12 Meronym Arg1:T57 Arg2:T58
R13 Meronym Arg1:T61 Arg2:T60
R14 Meronym Arg1:T67 Arg2:T66
R15 Mod-Link Arg1:T67 Arg2:T65
R16 Meronym Arg1:T71 Arg2:T73
R17 Mod-Link Arg1:T71 Arg2:T72
R18 Mod-Link Arg1:E21 Arg2:T77
R19 Setting Arg1:E23 Arg2:T80
T90 Time 1133 1138 daily
R20 Setting Arg1:E26 Arg2:T90
R21 Mod-Link Arg1:T84 Arg2:T85
R22 Meronym Arg1:T83 Arg2:T84
R23 Meronym Arg1:T86 Arg2:T88
R24 Mod-Link Arg1:T88 Arg2:T87