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protocol_166.ann
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T1 Action 40 47 Prepare
E1 Action:T1 Acts-on:T13 Site:T14
T2 Action 208 213 Rinse
E2 Action:T2 Acts-on:T26 Using:T27
T3 Action 258 263 Place
E3 Action:T3 Acts-on:T31 Site:T33
T4 Action 376 381 layer
E4 Action:T4 Using:T36 Using2:T35 Acts-on:T39 Site:T40
T5 Action 479 489 Centrifuge
E5 Action:T5
T6 Action 803 810 Air dry
E6 Action:T6 Acts-on:T51 Site:T52
T7 Action 571 579 pull off
E7 Action:T7 Acts-on:T47 Using:T45
T8 Action 605 613 Pull off
E8 Action:T8 Acts-on:T48 Acts-on2:T50
T9 Action 853 854 A
E9 Action:T9 Acts-on:T57
T10 Action 907 916 resuspend
E10 Action:T10 Acts-on:T58 Using:E9
T11 Action 985 992 extract
E11 Action:T11 Acts-on:T60
T12 Action 955 975 Perform virus counts
E12 Action:T12
T13 Reagent 48 55 sucrose
T14 Reagent 121 130 SM buffer
T15 Action 198 206 filtered
E13 Action:T15 Acts-on:T14
T16 Modifier 192 197 0.2μm
T17 Reagent 138 142 NaCl
T18 Reagent 148 153 MgSO4
T19 Reagent 160 168 TrisJHCl
T20 pH 170 176 pH 7.5
T21 Concentration 132 137 100mM
T22 Concentration 144 147 8mM
T23 Concentration 155 159 50mM
T24 Concentration 95 117 38% (weight to volume)
T25 Modifier 57 80 Molecular Biology Grade
T26 Location 214 224 SW40 tubes
T27 Reagent 230 239 SM buffer
T28 Reagent 243 256 sterile water
T29 Amount 268 273 9.5ml
T30 Modifier 274 289 DNase I-treated
T31 Reagent 290 296 sample
T32 Modifier 306 312 rinsed
T33 Location 313 333 ultracentrifuge tube
T34 Modifier 343 346 3cc
T35 Device 347 354 syringe
T36 Device 359 366 cannula
T37 Amount 386 392 2.5 ml
T38 Concentration 396 399 38%
T39 Reagent 400 407 sucrose
T40 Location 408 424 under the sample
T41 Speed 493 502 ~175,000g
T42 Time 507 514 3.25 hr
T43 Time 518 522 18°C
T44 Device 532 548 sterile transfer
T45 Device 552 569 serological pipet
T46 Amount 584 590 9.5 ml
T47 Reagent 591 597 sample
T48 Reagent 618 627 interface
T49 Amount 632 638 2.5 ml
T50 Reagent 639 646 sucrose
T51 Reagent 815 822 pellets
T52 Device 828 837 fume hood
T53 Time 842 851 15-20 min
T54 Reagent 894 898 EDTA
T55 Reagent 899 903 EGTA
T56 Concentration 884 888 0.1M
T57 Reagent 859 872 mixture of TE
T58 Reagent 921 928 pellets
T59 Amount 930 936 500 μl
T60 Reagent 993 996 DNA
R1 Mod-Link Arg1:T13 Arg2:T25
R2 Measure Arg1:T13 Arg2:T24
R3 Measure Arg1:T17 Arg2:T21
R4 Measure Arg1:T18 Arg2:T22
R5 Measure Arg1:T19 Arg2:T23
R6 Measure Arg1:T14 Arg2:T20
R7 Meronym Arg1:T19 Arg2:T14
R8 Meronym Arg1:T18 Arg2:T14
R9 Meronym Arg1:T17 Arg2:T14
R10 Mod-Link Arg1:E13 Arg2:T16
R11 Or Arg1:T27 Arg2:T28
R12 Mod-Link Arg1:T31 Arg2:T30
R13 Measure Arg1:T31 Arg2:T29
R14 Mod-Link Arg1:T33 Arg2:T32
R15 Mod-Link Arg1:T35 Arg2:T34
R16 Measure Arg1:T39 Arg2:T38
R17 Measure Arg1:T39 Arg2:T37
R18 Setting Arg1:E5 Arg2:T41
R19 Setting Arg1:E5 Arg2:T42
R20 Setting Arg1:E5 Arg2:T43
R21 Or Arg1:T45 Arg2:T44
R22 Measure Arg1:T50 Arg2:T49
R23 Setting Arg1:E6 Arg2:T53
R24 Measure Arg1:T54 Arg2:T56
R25 Measure Arg1:T55 Arg2:T56
R26 Meronym Arg1:T54 Arg2:T57
R27 Meronym Arg1:T55 Arg2:T57
R28 Measure Arg1:T57 Arg2:T59