-
Notifications
You must be signed in to change notification settings - Fork 3
/
Copy pathprotocol_182.ann
83 lines (83 loc) · 2.18 KB
/
protocol_182.ann
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
T1 Action 45 48 Add
E1 Action:T1 Acts-on:T20 Site:T22
T2 Action 92 95 Mix
E2 Action:T2
T3 Action 100 108 incubate
E3 Action:T3
T4 Action 125 133 Separate
E4 Action:T4 Using:T24
T5 Action 153 161 Transfer
E5 Action:T5 Acts-on:T26 Site:T28
T6 Action 223 226 Add
E6 Action:T6 Acts-on:T30
T7 Action 249 252 Mix
E7 Action:T7
T8 Action 257 265 incubate
E8 Action:T8
T9 Action 282 290 Separate
E9 Action:T9 Using:T32
T10 Action 309 316 discard
E10 Action:T10 Acts-on:T34
T11 Action 330 334 Wash
E11 Action:T11 Acts-on:T35 Using:T38
T12 Action 365 369;375 378 Move off
E12 Action:T12 Acts-on:T40
T13 Action 387 390 Dry
E13 Action:T13 Acts-on:T41
T14 Action 415 418 Add
E14 Action:T14 Acts-on:T44
T15 Action 429 432 Mix
E15 Action:T15
T16 Action 437 445 incubate
E16 Action:T16
T17 Action 461 469 Separate
E17 Action:T17 Using:T46
T18 Action 484 492 transfer
E18 Action:T18 Acts-on:T47 Site:T48
T19 Amount 49 54 65 µl
T20 Reagent 55 72 SPRI Ampure beads
R1 Measure Arg1:T20 Arg2:T19
T21 Amount 76 80 50µl
T22 Reagent 84 90 sample
R2 Measure Arg1:T22 Arg2:T21
T23 Time 113 123 20 minutes
R3 Setting Arg1:E2 Arg2:T23
R4 Setting Arg1:E3 Arg2:T23
T24 Device 137 143 magnet
T25 Time 145 150 6 min
R5 Setting Arg1:E4 Arg2:T25
T26 Reagent 166 177 supernatant
T27 Amount 185 191 115 µl
R6 Measure Arg1:T26 Arg2:T27
T28 Location 200 208 new well
T29 Amount 227 233 100 µl
T30 Reagent 237 247 SPRI beads
R7 Measure Arg1:T30 Arg2:T29
T31 Time 270 280 15 minutes
R8 Setting Arg1:E7 Arg2:T31
R9 Setting Arg1:E8 Arg2:T31
T32 Device 294 300 magnet
T33 Time 302 306 6min
R10 Setting Arg1:E9 Arg2:T33
T34 Reagent 317 328 supernatant
T35 Reagent 335 340 beads
T36 Amount 346 351 60 µl
T37 Concentration 355 358 70%
T38 Reagent 359 363 EtOH
R11 Measure Arg1:T38 Arg2:T37
R12 Measure Arg1:T38 Arg2:T36
T39 Modifier 370 374 well
T40 Device 379 385 magnet
R13 Mod-Link Arg1:E12 Arg2:T39
T41 Reagent 391 396 beads
T43 Time 406 412 ~10min
R14 Setting Arg1:E13 Arg2:T43
T42 Amount 419 424 40 µl
T44 Reagent 425 427 EB
R15 Measure Arg1:T44 Arg2:T42
T45 Time 450 459 3 minutes
R16 Setting Arg1:E15 Arg2:T45
R17 Setting Arg1:E16 Arg2:T45
T46 Device 473 479 magnet
T47 Reagent 493 507 eluted product
T48 Location 513 530 destination plate