-
Notifications
You must be signed in to change notification settings - Fork 3
/
Copy pathprotocol_200.ann
171 lines (171 loc) · 5.35 KB
/
protocol_200.ann
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
T1 Action 67 76 collected
E1 Action:T1 Acts-on:T24
T2 Action 188 197 collected
E2 Action:T2 Acts-on:T26 Site:T29
T3 Action 236 242 stored
E3 Action:T3 Acts-on:T26
T4 Action 331 338 removed
E4 Action:T4 Acts-on:T32 Acts-on2:T33 Using:T34
T5 Action 417 425;435 446 screened for viruses
E5 Action:T5 Acts-on:T38 Using:T39
T6 Action 826 830 made
E6 Action:T6 Using:T42 Creates:T40
T7 Action 858 868 solidified
E7 Action:T7 Acts-on:T40 Using:T44
T8 Action 936 944 examined
E8 Action:T8 Acts-on:T45
T9 Action 1044 1050 stored
E9 Action:T9 Acts-on:T46
T10 Action 1130 1136 warmed
E10 Action:T10 Acts-on:T46
T11 Action 1239 1244 mixed
E11 Action:T11 Acts-on:T55 Acts-on2:T58
T12 Action 1362 1367 added
E12 Action:T12 Acts-on:T63 Acts-on2:T61
T13 Action 1386 1391 mixed
E13 Action:T13 Acts-on:T64
T14 Action 1403 1409 poured
E14 Action:T14 Site:T67 Acts-on:T64
T15 Action 1491 1500 incubated
E15 Action:T15 Acts-on:T71
T16 Action 1514 1522 inverted
E16 Action:T16 Acts-on:T71 Site:T73
T17 Action 1581 1582;1596 1607 c for plaques
E17 Action:T17 Acts-on:T71
T18 Action 1633 1639 picked
E18 Action:T18 Using:T79 Acts-on:T78
T19 Action 1742 1753 transferred
E19 Action:T19 Acts-on:T81 Site:T82
T20 Action 1909 1917 vortexed
E20 Action:T20
T21 Action 1971 1980 replaqued
E21 Action:T21 Acts-on:T100 Site:T101
T22 Action 2002 2008 purify
E22 Action:T22 Acts-on:T102 Using:E21
T23 Action 2029 2038 eliminate
E23 Action:T23 Acts-on:T103 Using:E21
T24 Reagent 45 62 Salt lake samples
T25 Reagent 82 100 hypersaline waters
T26 Reagent 176 183 Samples
R1 Meronym Arg1:T24 Arg2:T25
T27 Modifier 201 208 sterile
T28 Size 209 216 5–10 mL
T29 Location 217 224 vessels
T30 Time 247 260 several weeks
T31 Temperature 264 280 room temperature
R2 Mod-Link Arg1:T29 Arg2:T27
R3 Measure Arg1:T29 Arg2:T28
R4 Setting Arg1:E3 Arg2:T30
R5 Setting Arg1:E3 Arg2:T31
T32 Reagent 301 306 cells
T33 Reagent 311 326 cellular debris
T34 Method 342 356 centrifugation
T35 Speed 358 364 5,000g
T36 Time 366 372 10 min
T37 Temperature 374 390 room temperature
T38 Reagent 397 408 supernatant
T39 Method 450 462 plaque assay
T40 Reagent 790 813 Base and overlay plates
T41 Size 815 820 90 mm
T42 Reagent 836 839 MGM
R6 Measure Arg1:T40 Arg2:T41
T43 Concentration 875 878 15%
T44 Reagent 879 888 w/v agar.
R7 Measure Arg1:T44 Arg2:T43
T45 Reagent 900 925 salt water concentrations
T46 Reagent 1030 1036 Plates
T47 Time 1051 1063 indefinitely
T48 Temperature 1067 1070 4°C
T49 Action 1072 1079 wrapped
E24 Action:T49 Using:T50
T50 Seal 1083 1090 plastic
T51 Action 1094 1113 prevent dessication
E25 Action:T51 Using:E24 Acts-on:T46
R8 Setting Arg1:E9 Arg2:T47
R9 Setting Arg1:E9 Arg2:T48
T52 Temperature 1140 1156 room temperature
T53 Temperature 1160 1166 warmer
R10 Or Arg1:T52 Arg2:T53
R11 Setting Arg1:E10 Arg2:T52
T54 Amount 1197 1207 100–500 µl
T55 Reagent 1215 1235 cleared water sample
T56 Amount 1250 1256 150 µl
T57 Modifier 1260 1281 exponentially growing
T58 Reagent 1282 1292 host cells
R12 Mod-Link Arg1:T58 Arg2:T57
R13 Measure Arg1:T58 Arg2:T56
R14 Measure Arg1:T55 Arg2:T54
T59 Amount 1300 1306 3–4 mL
T60 Temperature 1310 1323 molten (50°C)
T61 Reagent 1324 1337 top-layer MGM
T62 Concentration 1344 1348 0.7%
T63 Reagent 1349 1357 w/v agar
T64 Reagent 1377 1385 solution
T65 Modifier 1392 1398 gently
R15 Measure Arg1:T61 Arg2:T59
R16 Setting Arg1:E12 Arg2:T60
R17 Measure Arg1:T63 Arg2:T62
R18 Mod-Link Arg1:E13 Arg2:T65
T66 Modifier 1410 1416 evenly
T67 Location 1426 1431 plate
R19 Mod-Link Arg1:E14 Arg2:T66
T68 Action 1439 1446 setting
E26 Action:T68 Acts-on:T71 Site:T69
T69 Location 1452 1465 level surface
T70 Time 1470 1478 5–10 min
T71 Location 1480 1486 plates
T72 Modifier 1501 1512 aerobically
T73 Location 1526 1545 airtight containers
T74 Temperature 1549 1553 30°C
T75 Temperature 1558 1562 37°C
T76 Time 1567 1572 1–4 d
T77 Time 1586 1595 every day
R20 Setting Arg1:E17 Arg2:T77
R21 Setting Arg1:E26 Arg2:T70
R22 Mod-Link Arg1:E15 Arg2:T72
R23 Setting Arg1:E16 Arg2:T74
R24 Setting Arg1:E16 Arg2:T75
R25 Setting Arg1:E16 Arg2:T76
T78 Reagent 1609 1628 Any visible plaques
T79 Device 1646 1676 sterile glass Pasteur pipettes
T80 Device 1681 1714 sterile plastic micropipette tips
R26 Or Arg1:T79 Arg2:T80
T81 Reagent 1722 1732 agar plugs
T82 Location 1757 1762 tubes
T83 Amount 1774 1780 500 µl
T84 Reagent 1784 1801 halovirus diluent
T85 Concentration 1803 1809 2.47 M
T86 Reagent 1811 1815 NaCl
T87 Concentration 1817 1822 90 mM
T88 Reagent 1824 1829 MgCl2
T89 Concentration 1831 1836 90 mM
T90 Reagent 1838 1843 MgSO4
T91 Concentration 1845 1850 60 mM
T92 Reagent 1852 1855 KCl
T93 Concentration 1857 1861 3 mM
T94 Reagent 1863 1868 CaCl2
T95 Concentration 1870 1875 10 mM
T96 Reagent 1877 1885 Tris-HCl
T97 pH 1887 1893 pH 7.5
R27 Measure Arg1:T86 Arg2:T85
R28 Measure Arg1:T88 Arg2:T87
R29 Measure Arg1:T90 Arg2:T89
R30 Measure Arg1:T92 Arg2:T91
R31 Measure Arg1:T94 Arg2:T93
R32 Measure Arg1:T96 Arg2:T95
R33 Measure Arg1:T96 Arg2:T97
R34 Measure Arg1:T84 Arg2:T83
R35 Meronym Arg1:T86 Arg2:T84
R36 Meronym Arg1:T88 Arg2:T84
R37 Meronym Arg1:T90 Arg2:T84
R38 Meronym Arg1:T92 Arg2:T84
R39 Meronym Arg1:T94 Arg2:T84
R40 Meronym Arg1:T96 Arg2:T84
R41 Meronym Arg1:T84 Arg2:T82
T98 Action 1921 1931 homogenize
E27 Action:T98 Acts-on:T99 Using:E20
T99 Reagent 1936 1942 sample
T100 Reagent 1950 1961 suspensions
T101 Location 1984 1998 overlay plates
T102 Reagent 2013 2021 isolates
T103 Reagent 2039 2054 “false plaques”