protocol_220.ann

T1	Action 61 64	Fix
E1	Action:T1 Acts-on:T2 Using:T5
T2	Reagent 65 89	freshly dissected tissue
T3	Amount 91 101	<3mm thick
T4	Concentration 108 111	10%
T5	Reagent 112 120	formalin
T6	Reagent 124 139	other fixatives
T7	Time 144 151	24-48 h
T8	Temperature 155 171	room temperature
R1	Measure Arg1:T2 Arg2:T3	
R2	Measure Arg1:T5 Arg2:T4	
R3	Or Arg1:T5 Arg2:T6	
R4	Setting Arg1:E1 Arg2:T7	
R5	Setting Arg1:E1 Arg2:T8	
T9	Action 173 178	Rinse
E2	Action:T9 
T10	Reagent 183 189	tissue
T11	Reagent 195 212	running tap water
T12	Time 217 220	1 h
T13	Action 222 231	Dehydrate
E3	Action:T13 Acts-on:T14 Using:T16
T14	Reagent 236 242	tissue
T15	Concentration 251 254	70%
T16	Reagent 255 262	alcohol
T17	Time 264 270	45 min
R6	Measure Arg1:T16 Arg2:T15	
R7	Setting Arg1:E3 Arg2:T17	
T18	Action 272 277	Clear
E4	Action:T18 Acts-on:T19 Using:T20
T19	Reagent 282 288	tissue
T20	Reagent 309 315	xylene
T21	Time 317 320	1 h
T22	Action 329 334	Clear
E5	Action:T22 Acts-on:T23 Using:T24
T23	Reagent 339 345	tissue
T24	Reagent 366 372	xylene
T25	Time 374 377	1 h
R8	Setting Arg1:E4 Arg2:T21	
R9	Setting Arg1:E5 Arg2:T25	
T26	Action 386 393	Immerse
E6	Action:T26 Acts-on:T27 Using:T28
T27	Reagent 398 404	tissue
T28	Reagent 420 428	paraffin
T29	Time 430 433	1 h
T30	Time 486 489	1 h
T31	Time 542 545	1 h
T32	Reagent 476 484	paraffin
T33	Reagent 532 540	paraffin
T34	Action 442 449	Immerse
E7	Action:T34 Acts-on:T36 Using:T32
T35	Action 498 505	Immerse
E8	Action:T35 Acts-on:T37 Using:T33
T36	Reagent 454 460	tissue
T37	Reagent 510 516	tissue
R10	Setting Arg1:E8 Arg2:T31	
R11	Setting Arg1:E7 Arg2:T30	
R12	Setting Arg1:E6 Arg2:T29	
T38	Action 554 559	Embed
E9	Action:T38 Acts-on:T39 Site:T40
T39	Reagent 564 570	tissue
T40	Reagent 576 590	paraffin block
T41	Action 592 599	Section
E10	Action:T41 Acts-on:T42 Site:T43
T42	Reagent 604 634	paraffin-embedded tissue block
T43	Location 660 669	microtome
T44	Modifier 638 654	5-8 µm thickness
T45	Action 674 679	float
E11	Action:T45 Site:T47
T46	Temperature 685 689	40°C
T47	Location 690 700	water bath
T48	Reagent 712 727	distilled water
R13	Mod-Link Arg1:E10 Arg2:T44	
R14	Setting Arg1:E11 Arg2:T46	
R15	Meronym Arg1:T48 Arg2:T47	
T49	Action 729 737	Transfer
E12	Action:T49 Acts-on:T50 Site:T51
T50	Reagent 742 750	sections
T51	Location 756 768	glass slides
T52	Modifier 769 802	suitable for immunohistochemistry
R16	Mod-Link Arg1:T51 Arg2:T52	
T53	Action 827 832;844 850	Allow to dry
E13	Action:T53 Acts-on:T56
T54	Time 851 860	overnight
T55	Action 865 870	store
E14	Action:T55 Acts-on:T57
T56	Location 837 843	slides
T57	Location 871 877	slides
T58	Temperature 881 897	room temperature
R17	Setting Arg1:E13 Arg2:T54	
R18	Setting Arg1:E14 Arg2:T58	
T59	Time 898 917	until ready for use
R19	Setting Arg1:E14 Arg2:T59	
T60	Action 919 932	Deparaffinize
E15	Action:T60 Acts-on:T63 Using:T67
T61	Action 977 990	Deparaffinize
E16	Action:T61 Acts-on:T64 Using:T68
T62	Action 1035 1044	Drain off
E17	Action:T62 Acts-on:T65 Site:T66
T63	Location 933 939	slides
T64	Location 991 997	slides
T65	Reagent 1049 1064	blocking buffer
T66	Location 1074 1080	slides
T67	Reagent 955 961	xylene
T68	Reagent 1013 1019	xylene
T69	Time 963 968	5 min
T70	Time 1021 1026	5 min
T71	Action 1082 1090	Transfer
E18	Action:T71 Acts-on:T72 Site:T74
T72	Reagent 1091 1097	slides
T73	Concentration 1101 1105	100%
T74	Reagent 1106 1113	alcohol
T75	Time 1121 1129	3-minute
T76	Time 1184 1192	3-minute
T77	Reagent 1169 1176	alcohol
T78	Concentration 1164 1168	100%
T79	Reagent 1154 1160	slides
T80	Action 1145 1153	Transfer
E19	Action:T80 Acts-on:T79 Site:T77
R20	Measure Arg1:T74 Arg2:T73	
R21	Setting Arg1:E18 Arg2:T75	
R22	Measure Arg1:T77 Arg2:T78	
R23	Setting Arg1:E19 Arg2:T76	
T82	Action 1248 1258	incubating
E21	Action:T82 Acts-on:T83 Site:T85
T83	Reagent 1259 1267	sections
T84	Concentration 1271 1273	3%
T85	Reagent 1274 1287	H2O2 solution
T86	Reagent 1291 1299	methanol
T87	Temperature 1303 1319	room temperature
T88	Time 1324 1330	10 min
R24	Measure Arg1:T85 Arg2:T84	
R26	Setting Arg1:E21 Arg2:T87	
R27	Setting Arg1:E21 Arg2:T88	
T81	Action 1372 1378	Change
E20	Action:T81 Using:E23
T89	Action 1426 1432	Change
E22	Action:T89 Using:E24
T90	Action 1382 1389	rinsing
E23	Action:T90 Using:T94
T91	Action 1436 1443	rinsing
E24	Action:T91 Using:T95
T92	Amount 1393 1399	300 ml
T93	Amount 1447 1453	300 ml
T94	Reagent 1403 1406	PBS
T95	Reagent 1457 1460	PBS
T96	Time 1412 1417	5 min
T97	Time 1466 1471	5 min
R28	Measure Arg1:T94 Arg2:T92	
R29	Measure Arg1:T95 Arg2:T93	
R30	Setting Arg1:E23 Arg2:T96	
R31	Setting Arg1:E24 Arg2:T97	
T98	Action 1491 1516	Perform antigen retrieval
E25	Action:T98 
T99	Action 1619 1626	Arrange
E26	Action:T99 Acts-on:T100 Site:T101
T100	Location 1631 1637	slides
T101	Location 1643 1661	staining container
T102	Action 1663 1667	Pour
E27	Action:T102 Acts-on:T105 Site:T107
T103	Amount 1668 1674	300 ml
T104	Concentration 1678 1683	10 mM
T105	Reagent 1684 1698	citrate buffer
T106	pH 1700 1706	pH 6.0
T107	Location 1716 1734	staining container
T108	Action 1739 1747	incubate
E28	Action:T108 Acts-on:T109
T109	Mention 1748 1750	it
R32	Coreference-Link Arg1:T109 Arg2:T105	
R33	Measure Arg1:T105 Arg2:T104	
R34	Measure Arg1:T105 Arg2:T103	
R35	Measure Arg1:T105 Arg2:T106	
T110	Temperature 1754 1762	95-100°C
T111	Time 1767 1773	10 min
R36	Setting Arg1:E28 Arg2:T110	
R37	Setting Arg1:E28 Arg2:T111	
T112	Action 1830 1836	Remove
E29	Action:T112 Acts-on:T113
T113	Location 1841 1859	staining container
T114	Temperature 1863 1879	room temperature
T115	Action 1884 1889;1901 1908	allow to cool
E30	Action:T115 Acts-on:T116
T116	Reagent 1894 1900	slides
T117	Time 1913 1919	20 min
R38	Setting Arg1:E30 Arg2:T117	
R39	Setting Arg1:E29 Arg2:T114	
T118	Action 1921 1927	Change
E31	Action:T118 Using:E33
T119	Action 1978 1984	Change
E32	Action:T119 Using:E34
T120	Action 1931 1938	rinsing
E33	Action:T120 Acts-on:T122 Using:T126
T121	Action 1988 1995	rinsing
E34	Action:T121 Acts-on:T123 Using:T127
T122	Location 1939 1945	slides
T123	Location 1996 2002	slides
T124	Amount 1949 1955	300 ml
T125	Amount 2006 2012	300 ml
T126	Reagent 1956 1959	PBS
T127	Reagent 2013 2016	PBS
T128	Time 1964 1969	5 min
T129	Time 2021 2026	5 min
R40	Measure Arg1:T126 Arg2:T124	
R41	Measure Arg1:T127 Arg2:T125	
R42	Setting Arg1:E33 Arg2:T128	
R43	Setting Arg1:E34 Arg2:T129	
T130	Action 2046 2049	Add
E35	Action:T130 Acts-on:T132 Site:T136
T131	Amount 2050 2056	100 µl
T132	Reagent 2057 2072	blocking buffer
T133	Concentration 2079 2082	10%
T134	Reagent 2083 2101	fetal bovine serum
T135	Reagent 2105 2108	PBS
R44	Measure Arg1:T134 Arg2:T133	
R45	Measure Arg1:T132 Arg2:T131	
T136	Location 2119 2127	sections
T137	Location 2135 2141	slides
T138	Action 2146 2154	incubate
E36	Action:T138 Site:T139
T139	Location 2160 2178	humidified chamber
T140	Temperature 2182 2198	room temperature
T141	Time 2203 2205	1h
R48	Meronym Arg1:T136 Arg2:T137	
R49	Setting Arg1:E36 Arg2:T140	
R50	Setting Arg1:E36 Arg2:T141	
T142	Action 2207 2212	Apply
E37	Action:T142 Acts-on:T145 Site:T150
T143	Amount 2213 2219	100 µl
T144	Concentration 2220 2241	appropriately diluted
T145	Reagent 2242 2258	primary antibody
T146	Reagent 2263 2287	antibody dilution buffer
T147	Concentration 2294 2298	0.5%
T148	Reagent 2299 2319	bovine serum albumin
T149	Reagent 2323 2326	PBS
R51	Measure Arg1:T148 Arg2:T147	
R25	Meronym Arg1:T86 Arg2:T85	
R52	Meronym Arg1:T146 Arg2:T145	
R53	Meronym Arg1:T149 Arg2:T148	
R54	Meronym Arg1:T148 Arg2:T146	
R46	Meronym Arg1:T135 Arg2:T134	
R47	Meronym Arg1:T134 Arg2:T132	
R55	Measure Arg1:T145 Arg2:T144	
R56	Measure Arg1:T145 Arg2:T143	
T150	Reagent 2335 2343	sections
T151	Location 2351 2357	slides
T152	Action 2362 2370	incubate
E38	Action:T152 Site:T153
T153	Location 2376 2394	humidified chamber
T154	Temperature 2398 2414	room temperature
T155	Time 2419 2422	1 h
R57	Setting Arg1:E38 Arg2:T154	
R58	Setting Arg1:E38 Arg2:T155	
R59	Meronym Arg1:T150 Arg2:T151	
T156	Action 2425 2431	Change
E39	Action:T156 Using:E41
T157	Action 2486 2492	Change
E40	Action:T157 Using:E42
T158	Action 2435 2442	washing
E41	Action:T158 Acts-on:T160 Site:T164
T159	Action 2496 2503	washing
E42	Action:T159 Acts-on:T161 Site:T165
T160	Location 2447 2453	slides
T161	Location 2508 2514	slides
T162	Amount 2457 2463	300 ml
T163	Amount 2518 2524	300 ml
T164	Reagent 2464 2467	PBS
T165	Reagent 2525 2528	PBS
T166	Time 2472 2477	5 min
T167	Time 2533 2538	5 min
R60	Measure Arg1:T164 Arg2:T162	
R61	Measure Arg1:T165 Arg2:T163	
R62	Setting Arg1:E41 Arg2:T166	
R63	Setting Arg1:E42 Arg2:T167	
T168	Action 2547 2552	Apply
E43	Action:T168 Acts-on:T171 Site:T173
T169	Amount 2553 2559	100 µl
T170	Concentration 2560 2581	appropriately diluted
T171	Reagent 2582 2613	biotinylated secondary antibody
T172	Reagent 2625 2649	antibody dilution buffer
T173	Reagent 2658 2666	sections
T174	Location 2674 2680	slides
T175	Action 2685 2693	incubate
E44	Action:T175 Site:T176
T176	Location 2699 2717	humidified chamber
T177	Temperature 2721 2737	room temperature
T178	Time 2742 2748	30 min
R64	Measure Arg1:T171 Arg2:T170	
R65	Measure Arg1:T171 Arg2:T169	
R66	Meronym Arg1:T172 Arg2:T171	
R67	Setting Arg1:E44 Arg2:T177	
R68	Setting Arg1:E44 Arg2:T178	
R69	Meronym Arg1:T173 Arg2:T174	
T179	Action 2750 2756	Change
E45	Action:T179 Using:E47
T180	Action 2807 2813	Change
E46	Action:T180 Using:E48
T181	Action 2760 2767	washing
E47	Action:T181 Acts-on:T183 Using:T187
T182	Action 2817 2824	washing
E48	Action:T182 Acts-on:T184 Using:T188
T183	Location 2768 2774	slides
T184	Location 2825 2831	slides
T185	Amount 2778 2784	300 ml
T186	Amount 2835 2841	300 ml
T187	Reagent 2785 2788	PBS
T188	Reagent 2842 2845	PBS
T189	Time 2793 2798	5 min
T190	Time 2850 2855	5 min
R70	Measure Arg1:T187 Arg2:T185	
R71	Measure Arg1:T188 Arg2:T186	
R72	Setting Arg1:E47 Arg2:T189	
R73	Setting Arg1:E48 Arg2:T190	
T191	Action 2864 2869	Apply
E49	Action:T191 Acts-on:T194 Using:T195 Site:T196
T192	Amount 2870 2876	100 µl
T193	Concentration 2877 2898	appropriately diluted
T194	Reagent 2899 2917	Sav-HRP conjugates
T195	Reagent 2929 2953	antibody dilution buffer
T196	Reagent 2962 2970	sections
T197	Location 2978 2984	slides
T198	Action 2989 2997	incubate
E50	Action:T198 Site:T199
T199	Location 3003 3021	humidified chamber
T200	Temperature 3025 3041	room temperature
T201	Time 3046 3052	30 min
T202	Action 3054 3079	keep protected from light
E51	Action:T202 
R74	Setting Arg1:E50 Arg2:T200	
R75	Setting Arg1:E50 Arg2:T201	
R76	Measure Arg1:T194 Arg2:T193	
R77	Measure Arg1:T194 Arg2:T192	
R78	Meronym Arg1:T196 Arg2:T197	
T203	Action 3082 3088	Change
E52	Action:T203 Using:E54
T204	Action 3139 3145	Change
E53	Action:T204 Using:E55
T205	Action 3092 3099	washing
E54	Action:T205 Acts-on:T207 Using:T211
T206	Action 3149 3156	washing
E55	Action:T206 Acts-on:T208 Using:T212
T207	Location 3100 3106	slides
T208	Location 3157 3163	slides
T209	Amount 3110 3116	300 ml
T210	Amount 3167 3173	300 ml
T211	Reagent 3117 3120	PBS
T212	Reagent 3174 3177	PBS
T213	Time 3125 3130	5 min
T214	Time 3182 3187	5 min
R79	Measure Arg1:T211 Arg2:T209	
R80	Measure Arg1:T212 Arg2:T210	
R81	Setting Arg1:E54 Arg2:T213	
R82	Setting Arg1:E55 Arg2:T214	
T215	Action 3196 3201	Apply
E56	Action:T215 Acts-on:T217 Site:T218
T216	Amount 3202 3208	100 µl
T217	Reagent 3209 3231	DAB substrate solution
T219	Concentration 3263 3268	0.05%
T220	Concentration 3275 3281	0.015%
T221	Reagent 3290 3293	PBS
T222	Reagent 3282 3286	H2O2
T223	Reagent 3269 3272	DAB
R83	Measure Arg1:T223 Arg2:T219	
R84	Measure Arg1:T222 Arg2:T220	
R85	Meronym Arg1:T223 Arg2:T217	
R86	Meronym Arg1:T222 Arg2:T217	
R87	Meronym Arg1:T221 Arg2:T222	
T218	Reagent 3302 3310	sections
T224	Location 3318 3324	slides
T225	Action 3328 3344	reveal the color
E57	Action:T225 Using:E56 Acts-on:T226
T226	Reagent 3348 3365	antibody staining
R88	Measure Arg1:T217 Arg2:T216	
R89	Meronym Arg1:T218 Arg2:T224	
T227	Action 3367 3394	Allow the color development
E58	Action:T227 
T228	Time 3399 3406	< 5 min
R90	Setting Arg1:E58 Arg2:T228	
T229	Action 3453 3459	Change
E59	Action:T229 Using:E63
T230	Action 3510 3516	Change
E60	Action:T230 Using:E62
T231	Action 3567 3573	Change
E61	Action:T231 Using:E64
T232	Action 3520 3527	washing
E62	Action:T232 Acts-on:T236 Using:T242
T233	Action 3463 3470	washing
E63	Action:T233 Acts-on:T235 Using:T241
T234	Action 3577 3584	washing
E64	Action:T234 Acts-on:T237 Using:T243
T235	Location 3471 3477	slides
T236	Location 3528 3534	slides
T237	Location 3585 3591	slides
T238	Amount 3481 3487	300 ml
T239	Amount 3538 3544	300 ml
T240	Amount 3595 3601	300 ml
T241	Reagent 3488 3491	PBS
T242	Reagent 3545 3548	PBS
T243	Reagent 3602 3605	PBS
T244	Time 3496 3501	2 min
T245	Time 3553 3558	2 min
T246	Time 3610 3615	2 min
R91	Measure Arg1:T241 Arg2:T238	
R92	Measure Arg1:T242 Arg2:T239	
R93	Measure Arg1:T243 Arg2:T240	
R94	Setting Arg1:E63 Arg2:T244	
R95	Setting Arg1:E62 Arg2:T245	
R96	Setting Arg1:E64 Arg2:T246	
T247	Action 3635 3647	Counterstain
E65	Action:T247 Acts-on:T248 Using:E66
T248	Location 3648 3654	slides
T249	Action 3658 3667	immersing
E66	Action:T249 Acts-on:T250 Site:T251
T250	Location 3668 3673	sides
T251	Reagent 3677 3688	Hematoxylin
T252	Reagent 3695 3713	Gill’s Hematoxylin
T253	Time 3719 3726	1-2 min
R97	Meronym Arg1:T252 Arg2:T251	
R98	Setting Arg1:E66 Arg2:T253	
T254	Action 3728 3733	Rinse
E67	Action:T254 Acts-on:T255 Using:T256
T255	Location 3738 3744	slides
T256	Reagent 3748 3765	running tap water
T257	Time 3770 3778	> 15 min
R99	Setting Arg1:E67 Arg2:T257	
T258	Action 3780 3789	Dehydrate
E68	Action:T258 Acts-on:T266 Using:T262
T259	Action 3859 3868	Dehydrate
E69	Action:T259 Acts-on:T267 Using:T263
T260	Concentration 3828 3831	95%
T261	Concentration 3907 3910	95%
T262	Reagent 3832 3839	alcohol
T263	Reagent 3911 3918	alcohol
T264	Time 3845 3850	5 min
T265	Time 3924 3929	5 min
T266	Reagent 3794 3807	tissue slides
T267	Reagent 3873 3886	tissue slides
R100	Measure Arg1:T262 Arg2:T260	
R101	Measure Arg1:T263 Arg2:T261	
R102	Setting Arg1:E68 Arg2:T264	
R103	Setting Arg1:E69 Arg2:T265	
T268	Action 3938 3943	Clear
E70	Action:T268 Acts-on:T271 Using:T274
T269	Action 4046 4051	Clear
E71	Action:T269 Acts-on:T272 Using:T275
T270	Action 4154 4159	Clear
E72	Action:T270 Acts-on:T273 Using:T276
T271	Reagent 3948 3961	tissue slides
T272	Reagent 4056 4069	tissue slides
T273	Reagent 4164 4177	tissue slides
T274	Reagent 3977 3983	xylene
T275	Reagent 4085 4091	xylene
T276	Reagent 4193 4199	xylene
T277	Action 3988 3997	coverslip
E73	Action:T277 Using:T280
T278	Action 4096 4105	coverslip
E74	Action:T278 Using:T281
T279	Action 4204 4213	coverslip
E75	Action:T279 Using:T282
T280	Reagent 4004 4021	mounting solution
T281	Reagent 4112 4129	mounting solution
T282	Reagent 4220 4237	mounting solution
T283	Reagent 4028 4036	Permount
T284	Reagent 4136 4144	Permount
T285	Reagent 4244 4252	Permount
R104	Meronym Arg1:T283 Arg2:T280	
R105	Meronym Arg1:T284 Arg2:T281	
R106	Meronym Arg1:T285 Arg2:T282	
T286	Action 4261 4278	Observe the color
E76	Action:T286 Acts-on:T287
T287	Reagent 4286 4303	antibody staining
T288	Reagent 4311 4326	tissue sections
R107	Meronym Arg1:T287 Arg2:T288	
T289	Action 4345 4354	Dehydrate
E77	Action:T289 Acts-on:T294 Using:T304
T290	Action 4395 4404	Dehydrate
E78	Action:T290 Acts-on:T295 Using:T305
T291	Action 4445 4454	Dehydrate
E79	Action:T291 Acts-on:T296 Using:T306
T292	Action 4512 4521	Dehydrate
E80	Action:T292 Acts-on:T297 Using:T307
T293	Action 4579 4588	Dehydrate
E81	Action:T293 Acts-on:T298 Using:T308
T294	Reagent 4359 4365	tissue
T295	Reagent 4409 4415	tissue
T296	Reagent 4459 4465	tissue
T297	Reagent 4526 4532	tissue
T298	Reagent 4593 4599	tissue
T299	Concentration 4374 4377	80%
T300	Concentration 4424 4427	95%
T301	Concentration 4486 4490	100%
T302	Concentration 4553 4557	100%
T303	Concentration 4620 4624	100%
T304	Reagent 4378 4385	alcohol
T305	Reagent 4428 4435	alcohol
T306	Reagent 4491 4498	alcohol
T307	Reagent 4558 4565	alcohol
T308	Reagent 4625 4632	alcohol
T309	Time 4387 4393	45 min
T310	Time 4437 4443	45 min
T311	Time 4500 4503	1 h
T312	Time 4567 4570	1 h
T313	Time 4634 4637	1 h
R108	Measure Arg1:T304 Arg2:T299	
R109	Measure Arg1:T305 Arg2:T300	
R110	Measure Arg1:T306 Arg2:T301	
R111	Measure Arg1:T307 Arg2:T302	
R112	Measure Arg1:T308 Arg2:T303	
R113	Setting Arg1:E77 Arg2:T309	
R114	Setting Arg1:E79 Arg2:T311	
R115	Setting Arg1:E78 Arg2:T310	
R116	Setting Arg1:E80 Arg2:T312	
R117	Setting Arg1:E81 Arg2:T313	
T314	Action 4646 4654	Transfer
E82	Action:T314 Using:T324
T315	Action 4686 4694	Transfer
E83	Action:T315 Using:T325
T316	Action 4726 4734	Transfer
E84	Action:T316 Using:T326
T317	Action 4766 4775	Dehydrate
E85	Action:T317 Acts-on:T319 Using:T329
T318	Action 4846 4855	Dehydrate
E86	Action:T318 Using:T330 Acts-on:T320
T319	Reagent 4780 4793	tissue slides
T320	Reagent 4860 4873	tissue slides
T321	Concentration 4663 4666	95%
T322	Concentration 4703 4706	70%
T323	Concentration 4743 4746	50%
T324	Reagent 4667 4674	alcohol
T325	Reagent 4707 4714	alcohol
T326	Reagent 4747 4754	alcohol
T327	Concentration 4814 4818	100%
T328	Concentration 4894 4898	100%
T329	Reagent 4819 4826	alcohol
T330	Reagent 4899 4906	alcohol
T331	Time 4679 4684	3 min
T332	Time 4719 4724	3 min
T333	Time 4759 4764	3 min
T334	Time 4832 4837	5 min
T335	Time 4912 4917	5 min
R118	Measure Arg1:T324 Arg2:T321	
R119	Measure Arg1:T325 Arg2:T322	
R120	Measure Arg1:T326 Arg2:T323	
R121	Measure Arg1:T329 Arg2:T327	
R122	Measure Arg1:T330 Arg2:T328	
R123	Setting Arg1:E86 Arg2:T335	
R124	Setting Arg1:E85 Arg2:T334	
R125	Setting Arg1:E84 Arg2:T333	
R126	Setting Arg1:E83 Arg2:T332	
R127	Setting Arg1:E82 Arg2:T331