-
Notifications
You must be signed in to change notification settings - Fork 3
/
Copy pathprotocol_228.ann
83 lines (83 loc) · 2.26 KB
/
protocol_228.ann
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
T1 Action 32 40 Submerge
E1 Action:T1 Acts-on:T2 Site:T3
T2 Reagent 41 63 protein acrylamide gel
T3 Reagent 67 87 coomassie blue stain
T4 Action 92 97 shake
E2 Action:T4
T5 Temperature 101 117 room temperature
T6 Time 122 131 two hours
R1 Setting Arg1:E2 Arg2:T5
R2 Setting Arg1:E2 Arg2:T6
T7 Action 133 141 Pour off
E3 Action:T7 Acts-on:T8 Site:T9
T8 Reagent 142 147 stain
T9 Location 155 161 bottle
T10 Action 194 198 wash
E4 Action:T10 Acts-on:T11 Using:T12
T11 Reagent 199 202 gel
T12 Reagent 208 213 water
T13 Action 220 224 pour
E5 Action:T13 Acts-on:T14
T14 Reagent 230 235 stain
T15 Action 243 248 shake
E6 Action:T15
T16 Temperature 252 268 room temperature
T17 Time 273 290 another two hours
T18 Amount 225 229 more
R3 Measure Arg1:T14 Arg2:T18
R4 Setting Arg1:E6 Arg2:T16
R5 Setting Arg1:E6 Arg2:T17
T19 Action 292 300 Pour off
E7 Action:T19 Acts-on:T20 Site:T21
T20 Reagent 301 306 stain
T21 Location 314 320 bottle
T22 Action 322 326 wash
E8 Action:T22 Acts-on:T23 Using:T24
T23 Reagent 327 330 gel
T24 Reagent 336 341 water
T25 Action 405 419 take a picture
E9 Action:T25
T26 Action 426 430 pour
E10 Action:T26 Acts-on:T28
T27 Amount 431 435 more
T28 Reagent 436 441 stain
T29 Action 449 454 shake
E11 Action:T29
T30 Temperature 458 474 room temperature
T31 Time 475 484 overnight
R6 Setting Arg1:E11 Arg2:T30
R7 Setting Arg1:E11 Arg2:T31
R8 Measure Arg1:T28 Arg2:T27
T32 Action 486 494 Pour off
E12 Action:T32
T33 Reagent 495 500 stain
T34 Action 505 519 take a picture
E13 Action:T34
T35 Reagent 527 540 protein bands
T36 Action 542 550 Submerge
E14 Action:T36 Acts-on:T37 Site:T38
T37 Reagent 551 554 gel
T38 Reagent 558 572 destain buffer
T39 Amount 574 579 50 mL
T40 Reagent 580 591 Acetic Acid
T41 Amount 593 599 100 mL
T42 Reagent 600 608 Methanol
T43 Amount 610 616 350 mL
T44 Reagent 617 622 Water
T45 Time 631 635 hour
T46 Action 640 645 shake
E15 Action:T46
T47 Temperature 649 665 room temperature
R9 Measure Arg1:T40 Arg2:T39
R10 Measure Arg1:T42 Arg2:T41
R11 Measure Arg1:T44 Arg2:T43
R12 Meronym Arg1:T40 Arg2:T38
R13 Meronym Arg1:T42 Arg2:T38
R14 Meronym Arg1:T44 Arg2:T38
R15 Setting Arg1:E14 Arg2:T45
R16 Setting Arg1:E15 Arg2:T47
T48 Action 667 671 Wash
E16 Action:T48 Acts-on:T49
T49 Reagent 677 682 water
T50 Action 688 701 repeat step 5
E17 Action:T50