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protocol_253.ann
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T1 Action 74 81 Agitate
E1 Action:T1 Acts-on:T2 Using:T5 Using2:T7
T2 Reagent 82 85 gel
T3 Numerical 86 91 twice
T4 Concentration 97 100 50%
T5 Reagent 101 105 MeOH
T6 Concentration 107 110 10%
T7 Reagent 111 122 acetic acid
T8 Time 137 143 15 min
T9 Action 148 153 Rinse
E2 Action:T9 Using:T12
T10 Time 155 160 1 min
T11 Amount 167 173 200 ml
T12 Reagent 174 176 DW
T13 Action 178 185 Agitate
E3 Action:T13 Using:T15 Using2:T17
T14 Concentration 189 192 10%
T15 Reagent 193 197 EtOH
T16 Concentration 199 201 5%
T17 Reagent 202 221 glacial acetic acid
T18 Time 236 241 6 min
T19 Action 243 250 Agitate
E4 Action:T19 Using:T21
T20 Modifier 254 263 distilled
T21 Reagent 264 269 water
T22 Action 271 278 Agitate
E5 Action:T22 Using:T27
T23 Numerical 279 284 twice
T24 Time 286 291 9 min
T25 Modifier 292 296 each
T26 Concentration 303 309 500 ml
T27 Reagent 310 312 DW
T28 Action 314 321 Agitate
E6 Action:T28 Using:T31 Acts-on:T34
T29 Amount 325 331 500 ml
T30 Concentration 335 342 20 mg/L
T31 Reagent 343 350 Na2S2O4
T32 Modifier 394 403 distilled
T33 Modifier 405 414 deionized
T34 Reagent 415 420 water
T35 Time 431 435 9min
T36 Action 453 461 Pour off
E7 Action:T36 Acts-on:T37
T37 Reagent 462 470 solution
T38 Action 472 479 Agitate
E8 Action:T38
T39 Time 484 489 9 min
T40 Action 491 496 Rinse
E9 Action:T40 Using:T42
T41 Time 497 503 30 sec
T42 Reagent 509 512 DDW
T43 Action 516 522 remove
E10 Action:T43 Acts-on:T45
T44 Modifier 523 529 excess
T45 Reagent 530 535 AgNO3
T46 Action 537 540 Add
E11 Action:T46 Acts-on:T48 Acts-on2:T57
T47 Amount 541 547 200 ml
T48 Reagent 548 563 image developer
T49 Amount 565 568 1ml
T50 Concentration 569 572 37%
T51 Reagent 573 585 formaldehyde
T52 Concentration 599 601 3%
T53 Reagent 602 618 sodium carbonate
T55 Amount 631 637 200 ml
T56 Concentration 638 643 10g/L
T57 Reagent 644 662 sodium thiosulfate
T58 Action 664 672 Pour off
E13 Action:T58 Acts-on:T59
T59 Reagent 673 682 developer
T60 Action 684 687 Add
E14 Action:T60 Acts-on:T62
T61 Amount 688 693 80 ml
T62 Reagent 697 710 stop solution
T63 Amount 712 716 50 g
T64 Reagent 717 721 tris
T65 Amount 723 728 25 ml
T66 Reagent 729 743 glacial acetic
T67 Modifier 757 766 distilled
T68 Reagent 767 772 water
T69 Action 775 780 Store
E15 Action:T69 Acts-on:T70 Site:T72
T70 Reagent 781 784 gel
T71 Concentration 788 791 10%
T72 Reagent 792 800 glycerol
T73 Reagent 806 818 bacteriostat
T74 Concentration 820 825 0.02%
T75 Reagent 826 830 NaN3
T76 Action 851 854 add
E16 Action:T76 Acts-on:T79
T77 Amount 855 861 200 ml
T78 Concentration 863 867 0.1%
T79 Reagent 868 873 AgNO3
T80 Amount 875 881 200 mg
T81 Reagent 886 889 DDW
T82 Action 891 894 Add
E17 Action:T82 Acts-on:T85
T83 Amount 895 901 150 ml
T84 Concentration 902 905 37%
T85 Reagent 906 918 formaldehyde
T86 Action 920 927 Agitate
E18 Action:T86
T87 Generic-Measure 931 957 desired staining intensity
T88 Time 959 969 3 to 6 min
R1 Setting Arg1:E1 Arg2:T3
R2 Measure Arg1:T5 Arg2:T4
R3 Measure Arg1:T7 Arg2:T6
R4 Setting Arg1:E1 Arg2:T8
R5 Setting Arg1:E2 Arg2:T10
R6 Measure Arg1:T12 Arg2:T11
R7 Setting Arg1:E3 Arg2:T18
R8 Measure Arg1:T15 Arg2:T14
R9 Measure Arg1:T17 Arg2:T16
R10 Mod-Link Arg1:T21 Arg2:T20
R11 Setting Arg1:E5 Arg2:T23
R12 Mod-Link Arg1:T24 Arg2:T25
R13 Setting Arg1:E5 Arg2:T24
R14 Measure Arg1:T27 Arg2:T26
R15 Measure Arg1:T31 Arg2:T30
R16 Measure Arg1:T31 Arg2:T29
R17 Mod-Link Arg1:T34 Arg2:T33
R18 Mod-Link Arg1:T34 Arg2:T32
R19 Setting Arg1:E6 Arg2:T35
R20 Setting Arg1:E8 Arg2:T39
R21 Setting Arg1:E9 Arg2:T41
R22 Mod-Link Arg1:T45 Arg2:T44
R23 Measure Arg1:T48 Arg2:T47
R24 Measure Arg1:T51 Arg2:T50
R25 Measure Arg1:T51 Arg2:T49
R26 Measure Arg1:T53 Arg2:T52
R27 Measure Arg1:T57 Arg2:T56
R28 Measure Arg1:T57 Arg2:T55
R29 Measure Arg1:T62 Arg2:T61
R30 Measure Arg1:T64 Arg2:T63
R31 Measure Arg1:T66 Arg2:T65
R32 Mod-Link Arg1:T68 Arg2:T67
R33 Measure Arg1:T72 Arg2:T71
R34 Measure Arg1:T75 Arg2:T74
R35 Measure Arg1:T79 Arg2:T78
R36 Measure Arg1:T79 Arg2:T77
R38 Measure Arg1:T85 Arg2:T84
R39 Measure Arg1:T85 Arg2:T83
R40 Meronym Arg1:T75 Arg2:T73
R41 Meronym Arg1:T73 Arg2:T72
R37 Measure Arg1:T79 Arg2:T80
R42 Meronym Arg1:T79 Arg2:T81
R43 Setting Arg1:E18 Arg2:T87
R44 Or Arg1:T87 Arg2:T88
T89 Modifier 586 589 per
T90 Amount 590 595 liter
R45 Mod-Link Arg1:T90 Arg2:T89
R46 Measure Arg1:T53 Arg2:T90
R47 Meronym Arg1:T51 Arg2:T48
R48 Meronym Arg1:T53 Arg2:T48
T54 Modifier 744 747 per
T91 Amount 748 753 liter
R49 Measure Arg1:T68 Arg2:T91
R50 Mod-Link Arg1:T91 Arg2:T54
R51 Meronym Arg1:T64 Arg2:T62
R52 Meronym Arg1:T66 Arg2:T62
R53 Meronym Arg1:T68 Arg2:T62