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protocol_265.ann
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T1 Action 129 135 Dilute
E1 Action:T1 Acts-on:T22 Site:T24
T2 Action 177 194 EB Electroporate
E2 Action:T2
T3 Action 362 365 mix
E3 Action:T3
T4 Action 377 385 incubate
E4 Action:T4
T5 Action 406 409 Add
E5 Action:T5
T6 Action 456 459 mix
E6 Action:T6
T7 Action 479 487 transfer
E7 Action:T7
T8 Action 528 541 Electroporate
E8 Action:T8
T9 Action 599 607 Transfer
E9 Action:T9
T10 Action 675 679 Wash
E10 Action:T10
T11 Action 737 745 Incubate
E11 Action:T11
T12 Action 775 780 Plate
E12 Action:T12
T13 Action 804 809 Plate
E13 Action:T13
T14 Action 880 888 Incubate
E14 Action:T14
T15 Action 913 920 Collect
E15 Action:T15
T16 Action 973 978 elute
E16 Action:T16
T17 Action 1071 1074 PCR
E17 Action:T17
T18 Action 1122 1126 Pool
E18 Action:T18
T19 Action 1228 1235 measure
E19 Action:T19
T20 Action 1265 1271 dilute
E20 Action:T20
T21 Action 1298 1301 Run
E21 Action:T21
T22 Reagent 141 152 sub-library
T23 Concentration 156 164 50 ng/ul
T24 Reagent 168 173 water
R1 Setting Arg1:E1 Arg2:T23