-
Notifications
You must be signed in to change notification settings - Fork 3
/
Copy pathprotocol_282.ann
137 lines (137 loc) · 3.94 KB
/
protocol_282.ann
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
T1 Action 75 78 add
E1 Action:T1 Acts-on:T26 Site:T23
T2 Action 111 114 Add
E2 Action:T2 Acts-on:T29
T3 Action 161 164 Add
E3 Action:T3 Acts-on:T31 Acts-on2:T34
T4 Action 211 214 Add
E4 Action:T4 Acts-on:T36
T5 Action 270 273 mix
E5 Action:T5 Acts-on:T41 Using:E22
T6 Action 330 337 Pipette
E6 Action:T6 Acts-on:T46
T7 Action 460 463 Add
E7 Action:T7 Acts-on:T49 Site:T50
T8 Action 853 856 add
E8 Action:T8 Acts-on:T56
T9 Action 889 892 Add
E9 Action:T9 Acts-on:T59
T10 Action 939 942 Add
E10 Action:T10 Acts-on:T61
T11 Action 954 957 Add
E11 Action:T11 Acts-on:T64
T12 Action 990 993 Add
E12 Action:T12 Acts-on:T66
T13 Action 1033 1036 mix
E13 Action:T13 Using:E24
T14 Action 1041 1049 initiate
E14 Action:T14 Using:T69
T15 Action 1087 1093 remove
E15 Action:T15 Acts-on:T73 Using:T74
T16 Action 1125 1129 fill
E16 Action:T16 Acts-on:T75
T17 Action 1164 1173 pipetting
E17 Action:T17 Acts-on:T78
T18 Action 1258 1263 place
E18 Action:T18 Acts-on:T79
T19 Action 1319 1322 Let
E19 Action:T19 Using:T80
T20 Action 1354 1367 store wrapped
E20 Action:T20 Using:T81 Using2:T82
T22 Amount 52 57 15 mL
T23 Location 58 73 centrifuge tube
T24 Amount 79 83 5 mL
T25 Concentration 84 86 2x
T26 Reagent 87 108 Separating Gel Buffer
R1 Measure Arg1:T26 Arg2:T25
R2 Measure Arg1:T26 Arg2:T24
R3 Measure Arg1:T23 Arg2:T22
T27 Amount 115 121 2.5 mL
T28 Concentration 122 125 40%
T29 Reagent 126 151 Acrylamide/bis-acrylamide
R4 Measure Arg1:T29 Arg2:T28
R5 Measure Arg1:T29 Arg2:T27
T30 Amount 165 171 2.5 mL
T31 Reagent 172 178 DI H2O
T32 Amount 179 184 50 uL
T33 Concentration 185 188 10%
T34 Reagent 189 208 Ammonium persulfate
R6 Measure Arg1:T34 Arg2:T33
R7 Measure Arg1:T34 Arg2:T32
R8 Measure Arg1:T31 Arg2:T30
T35 Amount 215 219 5 uL
T36 Reagent 220 225 TEMED
T37 Action 232 238 invert
E22 Action:T37 Acts-on:T38 Count:T39
T38 Location 239 243 tube
T39 Numerical 244 251 several
T40 Modifier 261 269 properly
T41 Reagent 274 288 all components
T42 Action 293 301 initiate
E23 Action:T42 Using:T43
T43 Method 306 328 polymerization process
R9 Measure Arg1:T36 Arg2:T35
R10 Mod-Link Arg1:E5 Arg2:T40
T44 Amount 338 342 1 mL
T45 Concentration 360 363 10%
T46 Reagent 364 386 separating gel mixture
T47 Modifier 343 352 at a time
R11 Mod-Link Arg1:E6 Arg2:T47
R12 Measure Arg1:T46 Arg2:T45
R13 Measure Arg1:T46 Arg2:T44
T48 Amount 466 482 miniature amount
T49 Reagent 486 503 isopropyl alcohol
T50 Location 507 510 top
T51 Reagent 519 533 separating gel
R14 Measure Arg1:T49 Arg2:T48
R15 Meronym Arg1:T50 Arg2:T51
T52 Amount 830 835 15 mL
T53 Location 836 851 centrifuge tube
T54 Amount 857 861 2 mL
T55 Concentration 865 867 2x
T56 Reagent 868 887 stacking gel buffer
R16 Measure Arg1:T56 Arg2:T55
R17 Measure Arg1:T56 Arg2:T54
R18 Measure Arg1:T53 Arg2:T52
T57 Amount 893 899 0.4 mL
T58 Concentration 900 903 40%
T59 Reagent 904 929 acrylamide/bis-acrylamide
R19 Measure Arg1:T59 Arg2:T58
R20 Measure Arg1:T59 Arg2:T57
T60 Amount 943 949 1.6 mL
T61 Reagent 950 953 H2O
T62 Amount 958 963 20 uL
T63 Concentration 964 967 10%
T64 Reagent 968 987 Ammonium Persulfate
R21 Measure Arg1:T64 Arg2:T63
R22 Measure Arg1:T64 Arg2:T62
R23 Measure Arg1:T61 Arg2:T60
T65 Amount 994 998 4 uL
T66 Reagent 999 1004 TEMED
T67 Action 1009 1015 invert
E24 Action:T67 Acts-on:T68
T68 Location 1016 1020 tube
T69 Method 1050 1064 polymerization
R24 Measure Arg1:T66 Arg2:T65
T70 Modifier 1024 1032 properly
R25 Mod-Link Arg1:E13 Arg2:T70
T71 Modifier 1094 1097 any
T72 Modifier 1098 1106 leftover
T73 Reagent 1107 1118 isopropanol
R26 Mod-Link Arg1:T73 Arg2:T72
R27 Mod-Link Arg1:T73 Arg2:T71
T74 Device 1072 1083 paper towel
T75 Reagent 1134 1137 gel
T76 Amount 1177 1181 1 mL
T77 Concentration 1189 1191 4%
T78 Reagent 1192 1204 stacking gel
R28 Measure Arg1:T78 Arg2:T77
R29 Measure Arg1:T78 Arg2:T76
T79 Device 1268 1272 comb
T80 Method 1323 1333 polymerize
T21 Modifier 1373 1376 wet
T81 Seal 1377 1388 paper towel
T82 Seal 1393 1400 plastic
T83 Temperature 1404 1407 4 C
R30 Mod-Link Arg1:T81 Arg2:T21
R31 Setting Arg1:E20 Arg2:T83