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protocol_288.ann
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T1 Action 48 51 Add
E1 Action:T1 Acts-on:T23 Site:T24
T2 Action 117 121 Melt
E2 Action:T2 Acts-on:T25 Site:T26
T3 Action 160 164 cool
E3 Action:T3
T4 Action 169 177 maintain
E4 Action:T4 Site:T28
T5 Action 203 207 Warm
E5 Action:T5 Acts-on:T29 Using:T31
T6 Action 274 277 mix
E6 Action:T6 Acts-on:T33
T7 Action 329 337 transfer
E7 Action:T7 Acts-on:T35 Site:T36
T8 Action 397 405 transfer
E8 Action:T8 Acts-on:T38 Site:T39
T9 Action 524 531 amended
E9 Action:T9 Acts-on:T42
T10 Action 583 591 Incubate
E10 Action:T10
T11 Action 623 629 Decant
E11 Action:T11 Acts-on:T45
T12 Action 695 700 Rinse
E12 Action:T12 Acts-on:T46 Count:T47
T13 Action 728 734 adding
E13 Action:T13 Acts-on:T50
T14 Action 780 790 incubating
E14 Action:T14
T15 Action 827 836 decanting
E15 Action:T15 Acts-on:T56
T16 Action 854 859 Rinse
E16 Action:T16 Acts-on:T57
T17 Action 912 920 incubate
E17 Action:T17
T18 Action 961 967 decant
E18 Action:T18 Acts-on:T63
T19 Action 996 1001 rinse
E19 Action:T19 Acts-on:T64
T20 Action 1013 1016 add
E20 Action:T20 Acts-on:T67
T21 Action 1054 1062 incubate
E21 Action:T21
T22 Action 1127 1132 Store
E22 Action:T22 Acts-on:T71 Site:T73
T23 Reagent 52 59 agarose
T24 Reagent 63 72 SE buffer
T25 Reagent 126 133 agarose
T26 Device 139 153 microwave oven
T27 Temperature 181 185 37°C
T28 Device 191 201 water bath
R1 Setting Arg1:E4 Arg2:T27
T29 Reagent 212 229 viral concentrate
T30 Temperature 233 237 37°C
T31 Device 245 255 water bath
T32 Modifier 262 273 immediately
T33 Reagent 302 316 molten agarose
T34 Modifier 321 328 quickly
T35 Reagent 342 349 mixture
T36 Location 353 366 casting molds
R2 Setting Arg1:E5 Arg2:T30
R3 Mod-Link Arg1:E6 Arg2:T32
T37 Amount 286 298 equal volume
R4 Measure Arg1:T33 Arg2:T37
R5 Mod-Link Arg1:E7 Arg2:T34
T38 Reagent 410 414 plug
T39 Location 482 486 tube
T40 Amount 498 507 5 volumes
T41 Reagent 511 523 lysis buffer
T42 Reagent 537 549 proteinase K
R6 Measure Arg1:T41 Arg2:T40
R7 Meronym Arg1:T39 Arg2:T41
T43 Temperature 595 611 room temperature
T44 Time 612 621 overnight
R8 Setting Arg1:E10 Arg2:T43
R9 Setting Arg1:E10 Arg2:T44
T45 Reagent 634 646 lysis buffer
T46 Reagent 705 710 plugs
T47 Numerical 711 716 twice
T48 Amount 735 745 25 volumes
T49 Modifier 749 754 fresh
T50 Reagent 755 757 TE
T51 Concentration 769 773 1 mM
T52 Reagent 774 778 PMSF
R10 Measure Arg1:T52 Arg2:T51
R11 Mod-Link Arg1:T50 Arg2:T49
R12 Measure Arg1:T50 Arg2:T48
R13 Meronym Arg1:T52 Arg2:T50
T53 Time 795 798 1 h
T54 Modifier 804 810 gentle
T55 Method 811 820 agitation
R14 Setting Arg1:E14 Arg2:T53
R15 Or Arg1:T55 Arg2:T54
T56 Reagent 841 852 rinse fluid
T57 Reagent 864 869 plugs
T58 Action 871 874 add
E23 Action:T58 Acts-on:T61
T59 Amount 875 885 50 volumes
T60 Modifier 889 894 fresh
T61 Reagent 895 910 TE with no PMSF
T62 Time 925 931 30 min
R16 Setting Arg1:E17 Arg2:T62
R17 Mod-Link Arg1:T61 Arg2:T60
R18 Measure Arg1:T61 Arg2:T59
T63 Reagent 972 983 rinse fluid
T64 Reagent 1006 1011 plugs
T65 Amount 1017 1027 50 volumes
T66 Modifier 1031 1036 fresh
T67 Reagent 1037 1052 TE with no PMSF
T68 Time 1067 1073 30 min
R19 Setting Arg1:E21 Arg2:T68
R20 Mod-Link Arg1:T67 Arg2:T66
R21 Measure Arg1:T67 Arg2:T65
T69 Action 1103 1109 decant
E24 Action:T69 Acts-on:T70
T70 Reagent 1114 1125 rinse fluid
T71 Reagent 1137 1142 plugs
T72 Temperature 1146 1149 4°C
T73 Reagent 1163 1165 TE
R22 Setting Arg1:E22 Arg2:T72
T74 Modifier 1150 1159 submerged
R23 Mod-Link Arg1:E22 Arg2:T74