protocol_314.ann

T1	Action 28 33	Place
E1	Action:T1 Acts-on:T7 Site:T8
T2	Action 103 115	Flash freeze
E2	Action:T2 Using:E6
T3	Action 210 216	Attach
E3	Action:T3 Site:T15
T4	Action 261 267	Scrape
E4	Action:T4 Acts-on:T17 Site:T19
T5	Action 315 318	Add
E5	Action:T5 Acts-on:T20
T6	Numerical 34 35	1
T7	Reagent 36 49	cup of coffee
T8	Location 80 100	lyophilization flask
T9	Modifier 73 78	clean
R1	Mod-Link Arg1:T8 Arg2:T9	
R2	Measure Arg1:T7 Arg2:T6	
T10	Action 119 126	bathing
E6	Action:T10 Acts-on:T11 Using:T12 Site:T13
T11	Location 127 132	flask
T12	Method 139 152	hand rotation
T13	Location 159 163	bath
T14	Reagent 167 182	liquid nitrogen
R3	Meronym Arg1:T14 Arg2:T13	
T15	Device 220 248	vacuum lyophilization system
T16	Time 249 258	overnight
R4	Setting Arg1:E3 Arg2:T16	
T17	Reagent 268 288	lyophilized crystals
T18	Modifier 296 301	clean
T19	Location 302 312	coffee cup
R5	Mod-Link Arg1:T19 Arg2:T18	
T20	Reagent 319 328	hot water