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protocol_330.ann
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T1 Action 49 54 Label
E1 Action:T1 Acts-on:T25
T2 Action 117 122 Label
E2 Action:T2 Acts-on:T26
T3 Action 279 282 put
E3 Action:T3 Acts-on:T29 Site:T32 Using:T35
T4 Action 370 378 dispense
E4 Action:T4 Acts-on:T39 Using:T37 Site:T41
T5 Action 437 443 Repeat
E5 Action:T5 Site:T42
T6 Action 558 565 pipette
E6 Action:T6 Acts-on:T49 Site:T44
T7 Action 587 590 mix
E7 Action:T7 Acts-on:T50 Using:E6
T8 Action 606 613 Replace
E8 Action:T8 Acts-on:T51
T9 Action 680 699 Pipette up and down
E9 Action:T9
T10 Action 723 730 Replace
E10 Action:T10 Acts-on:T58
T11 Action 783 789 Repeat
E11 Action:T11 Using:T12 Using2:T13 Using3:T18
T14 Action 962 967 pipet
E14 Action:T14 Acts-on:T70 Site:T71
T15 Action 1035 1040 Reset
E15 Action:T15 Acts-on:T73
T16 Action 1073 1079 put on
E16 Action:T16 Acts-on:T77 Site:T73
T17 Action 1097 1105 transfer
E17 Action:T17 Acts-on:T78 Site:T80
T19 Action 1203 1206 Put
E19 Action:T19 Site:T83 Acts-on:T82
T20 Action 1246 1251 place
E20 Action:T20 Site:T84 Acts-on:T83
T21 Action 1444 1447 add
E21 Action:T21 Using:T92 Acts-on:T95 Site:T98
T22 Action 1514 1518 Seal
E22 Action:T22 Using:T102 Acts-on:T101
T23 Action 1548 1554 return
E23 Action:T23 Acts-on:T103 Site:T104
T24 Action 1349 1355 adsorb
E24 Action:T24 Acts-on:T88 Site:T89
T25 Location 59 85 dilution preparation plate
T26 Location 134 140 plates
T27 Reagent 127 133 sample
R1 Meronym Arg1:T27 Arg2:T26
T28 Amount 283 289 270 µL
T29 Reagent 293 298 Pro99
T30 Reagent 302 311 SN medium
T31 Amount 315 318 all
T32 Location 319 324 wells
T33 Location 328 342 dilution plate
T34 Size 245 254 8x1500 µL
T35 Device 255 277 multi-channel pipettor
R2 Meronym Arg1:T29 Arg2:T30
R3 Measure Arg1:T29 Arg2:T28
R4 Measure Arg1:T30 Arg2:T28
R5 Measure Arg1:T32 Arg2:T31
R6 Meronym Arg1:T32 Arg2:T33
R7 Setting Arg1:T35 Arg2:T34
T36 Size 353 359 100 µL
T37 Device 360 368 pipettor
T38 Amount 379 384 30 µL
T39 Reagent 388 400 virus sample
T40 Location 419 433 dilution plate
T41 Location 406 411 row H
R8 Meronym Arg1:T41 Arg2:T40
R9 Measure Arg1:T39 Arg2:T38
R10 Setting Arg1:T37 Arg2:T36
T42 Reagent 465 478 virus samples
T43 Size 489 498 12x300 µL
T44 Device 499 521 multi-channel pipettor
T45 Size 529 534 30 µL
T46 Amount 540 542 12
T47 Device 543 547 tips
T48 Action 548 556 attached
E25 Action:T48 Acts-on:T47 Acts-on2:T44
R11 Setting Arg1:T47 Arg2:T46
R12 Setting Arg1:T44 Arg2:T45
R13 Setting Arg1:T44 Arg2:T43
T49 Location 566 571 row H
T50 Reagent 595 603 dilution
T51 Device 618 622 tips
T52 Action 627 634 pipette
E26 Action:T52 Acts-on2:T56 Site2:T55
T54 Location 646 651 row H
T55 Location 657 662 row A
T56 Mention 635 640 30 µL
R14 Meronym Arg1:T56 Arg2:T54
T53 Action 717 720 mix
E27 Action:T53 Using:E9
T57 Numerical 700 713 several times
R15 Setting Arg1:E9 Arg2:T57
T58 Device 735 739 tips
T59 Action 744 752 transfer
E28 Action:T59 Acts-on:T60 Site:T61
T60 Mention 753 758 30 µL
T61 Location 764 769 row B
T63 Numerical 835 836 4
T12 Method 795 801 mixing
T13 Method 803 817 replacing tips
T18 Method 822 834 transferring
R16 Setting Arg1:E11 Arg2:T63
T62 Size 894 903 12x300 µL
T64 Device 904 926 multi-channel pipettor
T65 Size 934 939 30 µL
T66 Amount 945 946 8
T67 Device 947 951 tips
T68 Action 952 960 attached
E12 Action:T68 Acts-on:T67 Site:T64
R17 Setting Arg1:T67 Arg2:T66
R18 Setting Arg1:T64 Arg2:T62
R19 Setting Arg1:T64 Arg2:T65
T69 Amount 968 973 30 µL
T70 Reagent 974 984 host cells
T71 Location 994 1011 appropriate wells
R20 Measure Arg1:T70 Arg2:T69
T72 Location 1019 1031 sample plate
R21 Meronym Arg1:T71 Arg2:T72
T73 Device 1045 1053 pipettor
T74 Size 1066 1071 20 µL
R22 Setting Arg1:T73 Arg2:T74
T75 Amount 1080 1081 8
T76 Modifier 1082 1087 fresh
T77 Device 1088 1092 tips
R23 Mod-Link Arg1:T77 Arg2:T76
R24 Setting Arg1:T77 Arg2:T75
T78 Mention 1106 1111 20 µL
T79 Location 1121 1141 virus dilution plate
T80 Location 1154 1174 corresponding column
T81 Location 1182 1199 sample test plate
R25 Meronym Arg1:T80 Arg2:T81
R26 Meronym Arg1:T78 Arg2:T79
T82 Seal 1211 1215 lids
T83 Location 1223 1241 sample test plates
T84 Device 1255 1264 incubator
T85 Temperature 1293 1304 temperature
T86 Modifier 1271 1282 appropriate
R27 Mod-Link Arg1:T85 Arg2:T86
R28 Setting Arg1:E20 Arg2:T85
T87 Generic-Measure 1283 1288 light
R29 Mod-Link Arg1:T87 Arg2:T86
R30 Setting Arg1:E20 Arg2:T87
T88 Reagent 1343 1348 virus
T89 Reagent 1363 1367 host
T90 Time 1372 1376 1 hr
R31 Setting Arg1:E24 Arg2:T90
T91 Size 1389 1398 8x1500 µL
T92 Device 1399 1421 multi-channel pipettor
T93 Size 1434 1442 6x200 µL
R32 Setting Arg1:T92 Arg2:T91
R33 Setting Arg1:T92 Arg2:T93
T94 Amount 1448 1454 200 µL
T95 Reagent 1458 1463 Pro99
T96 Reagent 1467 1476 SN medium
T97 Amount 1480 1483 all
T98 Location 1484 1489 wells
T99 Location 1497 1510 sample plates
T100 Amount 1493 1496 all
R34 Meronym Arg1:T98 Arg2:T99
R35 Measure Arg1:T99 Arg2:T100
R36 Measure Arg1:T98 Arg2:T97
R37 Meronym Arg1:T95 Arg2:T96
R38 Measure Arg1:T95 Arg2:T94
R39 Measure Arg1:T96 Arg2:T94
T101 Location 1524 1529 plate
T102 Seal 1535 1543 parafilm
T103 Location 1555 1561 plates
T104 Device 1569 1578 incubator