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protocol_380.ann
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T1 Action 33 36 Add
E1 Action:T1 Acts-on:T39 Site:T40
T2 Action 109 112 Add
E2 Action:T2 Acts-on:T19 Site:T20
T3 Action 173 179 Pellet
E3 Action:T3 Acts-on:T42 Site:T43
T5 Action 301 304 Add
E5 Action:T5 Acts-on:T44
T6 Action 329 335 vortex
E6 Action:T6
T7 Action 337 345 Incubate
E7 Action:T7 Using:E8
T8 Action 380 389 vortexing
E8 Action:T8
T9 Action 416 419 Add
E9 Action:T9 Acts-on:T28 Site:T29
T10 Action 447 454 pipette
E10 Action:T10 Acts-on:T28
T11 Action 468 478 centrifuge
E11 Action:T11 Acts-on:T29
T12 Action 509 515 Remove
E12 Action:T12 Acts-on:T32
T13 Action 536 545 resuspend
E13 Action:T13 Acts-on:T34 Site:T46
T14 Action 579 585 spread
E14 Action:T14 Acts-on:T34 Site:T35
T15 Action 610 618 incubate
E15 Action:T15 Acts-on:T34
T16 Amount 37 42 90 uL
T17 Amount 113 118 10 uL
T18 Amount 191 195 1 mL
T19 Reagent 128 143 Lithium Acetate
T20 Location 151 171 microcentrifuge tube
T21 Amount 80 86 1.5 mL
T22 Temperature 349 353 45°C
T25 Time 404 414 10 minutes
T26 Modifier 390 397 briefly
T27 Amount 420 424 1 mL
T28 Reagent 425 430 water
T29 Location 438 442 tube
T30 Speed 482 492 14,000 RPM
T31 Time 497 507 15 seconds
T32 Reagent 520 531 supernatant
T33 Amount 560 566 200 uL
T34 Reagent 550 556 pellet
T35 Location 589 605 selective plates
T36 Temperature 622 626 30°C
T37 Time 631 639 3-6 days
T38 Generic-Measure 46 49 50%
T39 Reagent 50 74 Polyethylene Glycol 3350
T40 Location 87 107 microcentrifuge tube
R1 Measure Arg1:T39 Arg2:T38
R2 Measure Arg1:T39 Arg2:T16
R3 Measure Arg1:T40 Arg2:T21
T41 Generic-Measure 122 127 0.2 N
R4 Measure Arg1:T19 Arg2:T41
R5 Measure Arg1:T19 Arg2:T17
T42 Reagent 180 185 yeast
T43 Location 196 210 liquid culture
R6 Measure Arg1:T43 Arg2:T18
T4 Amount 305 309 1 ug
T44 Reagent 313 316 DNA
T45 Modifier 321 328 briefly
R7 Measure Arg1:T44 Arg2:T4
R8 Mod-Link Arg1:E6 Arg2:T45
R9 Setting Arg1:E7 Arg2:T22
T23 Time 358 378 30 minutes to 1 hour
R10 Setting Arg1:E7 Arg2:T23
R11 Mod-Link Arg1:E8 Arg2:T26
R12 Setting Arg1:E8 Arg2:T25
T24 Modifier 398 403 every
R13 Mod-Link Arg1:E8 Arg2:T24
R14 Measure Arg1:T28 Arg2:T27
R15 Setting Arg1:E11 Arg2:T30
R16 Setting Arg1:E11 Arg2:T31
T46 Location 567 572 water
R17 Measure Arg1:T46 Arg2:T33
R18 Setting Arg1:E15 Arg2:T36
R19 Setting Arg1:E15 Arg2:T37