protocol_48.txt

LB Agar Plates
Weigh out the following into a 1L Erlenmeyer flask: 5g NaCl.
5g Tryptone.
2.5g Yeast Extract.
7.5g Agar.
add (dH2O) to 500mL.
Swirl to mix.
Cover the top of the flask with aluminum foil and label with autoclave tape.
Autoclave on the liquid setting for 20 minutes or according to your autoclave's specifications.
After removing the solution from the autoclave, allow the agar solution to cool to 55°C.
Add the appropriate amount of desired antibiotic to the solution (500μL if you are using a 1,000x antibiotic stock) and swirl to mix.
Pour ~20mL of LB agar per 10cm polystyrene Petri dish.
Place the lids on the plates and allow them to cool for 30-60 minutes (until solidified).
Invert the plates and let sit for several more hours or overnight.
Label the bottom of plates with antibiotic and date and store in plastic bags or sealed with parafilm at 4°C.