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protocol_510.ann
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T1 Action 45 54 spin down
E1 Action:T1 Acts-on:T151
T2 Action 132 136 Keep
E2 Action:T2
T3 Action 148 154 vortex
E3 Action:T3 Acts-on:T153
T4 Action 179 184 Allow
E4 Action:T4 Acts-on:T154
T5 Action 243 249 vortex
E5 Action:T5 Acts-on:T156
T6 Action 327 334 combine
E6 Action:T6 Acts-on:T157 Site:T159
T7 Action 559 562 Mix
E7 Action:T7 Using:T181
T8 Action 582 590 Incubate
E8 Action:T8 Using:T170
T9 Action 629 637 Incubate
E9 Action:T9 Acts-on:T173
T10 Action 673 677 Spin
E10 Action:T10 Acts-on:T176
T11 Action 735 738 Add
E11 Action:T11 Acts-on:T179 Site:T180
T12 Action 783 786 mix
E12 Action:T12 Acts-on:T180 Using:T182
T13 Action 806 814 Incubate
E13 Action:T13
T14 Action 850 855 Place
E14 Action:T14 Acts-on:T186 Site:T187
T15 Action 959 965 Remove
E15 Action:T15 Acts-on:T190
T16 Action 970 977 discard
E16 Action:T16 Acts-on:T190
T17 Action 1100 1103 add
E17 Action:T17 Acts-on:T197 Site2:T198
T18 Action 1176 1184 incubate
E18 Action:T18 Acts-on:T191
T19 Action 1238 1244 remove
E19 Action:T19 Acts-on:T200 Using:T201
T20 Action 1300 1303 add
E20 Action:T20 Acts-on:T206 Site:T207
T21 Action 1376 1384 incubate
E21 Action:T21 Acts-on:T208
T22 Action 1438 1444 remove
E22 Action:T22 Acts-on:T211 Using:T212
T23 Action 1502 1508 Remove
E23 Action:T23 Acts-on:T213 Site:T214
T24 Action 1545 1552 let dry
E24 Action:T24 Acts-on:T213
T25 Action 1614 1623 Resuspend
E25 Action:T25 Acts-on:T217 Using:T219
T26 Action 1669 1672 Mix
E26 Action:T26 Using:T221
T27 Action 1755 1763 Incubate
E27 Action:T27 Acts-on:T222
T28 Action 1817 1822 Place
E28 Action:T28 Acts-on:T225 Site:T226
T29 Action 1901 1909 transfer
E29 Action:T29 Acts-on:T230 Site:T231
T30 Action 2022 2028 repeat
E30 Action:T30 Using:T232 Acts-on:T234
T31 Action 2257 2265 Incubate
E31 Action:T31
T32 Action 2422 2425 Mix
E32 Action:T32 Using:T254
T33 Action 2445 2450 Apply
E33 Action:T33 Using:T255
T34 Action 2479 2484 place
E34 Action:T34 Site:T256
T35 Action 2532 2539 Prepare
E35 Action:T35 Creates:T257
T36 Action 2602 2605 Mix
E36 Action:T36
T37 Action 2610 2614 pour
E37 Action:T37 Site:T260
T38 Action 2630 2634 Load
E38 Action:T38 Acts-on:T262 Acts-on2:T264 Acts-on3:T266
T39 Action 2726 2730 Load
E39 Action:T39 Acts-on:T267 Site:T268 Acts-on2:T271
T40 Action 2805 2808 Run
E40 Action:T40 Acts-on:T272 Using:T273 Measure-Type-Link:T275
T41 Action 2909 2918 Visualize
E41 Action:T41 Acts-on:T277 Using:T278
T44 Action 3227 3230 Set
E44 Action:T44 Acts-on:T42
T45 Action 3253 3256 mix
E45 Action:T45 Using:T281
T46 Action 3276 3284 Transfer
E46 Action:T46 Acts-on:T282 Site:T283
T47 Action 3315 3321 Follow
E47 Action:T47 Using:T284
T48 Action 3529 3537 Transfer
E48 Action:T48 Acts-on:T285 Site:T286 Site2:T288
T49 Action 3646 3655 spin down
E49 Action:T49 Acts-on:T289 Site:T290
T50 Action 3948 3956 quantify
E50 Action:T50 Acts-on:T293 Using:T294
T51 Action 3997 4004 combine
E51 Action:T51 Acts-on:T295 Site:T297
T52 Action 4219 4222 Mix
E52 Action:T52 Using:T305
T53 Action 4242 4247 Apply
E53 Action:T53 Using:T306
T54 Action 4327 4330 Add
E54 Action:T54 Acts-on:T312 Site:T313
T55 Action 4375 4378 mix
E55 Action:T55 Using:T315
T56 Action 4398 4406 Incubate
E56 Action:T56 Acts-on:T316
T57 Action 4449 4454 Place
E57 Action:T57 Acts-on:T319 Site:T320
T58 Action 4573 4579 Remove
E58 Action:T58 Acts-on:T323
T59 Action 4584 4591 discard
E59 Action:T59 Acts-on:T323
T60 Action 4714 4717 add
E60 Action:T60 Acts-on:T328 Site:T329
T61 Action 4790 4798 incubate
E61 Action:T61 Acts-on:T330
T62 Action 4992 5000 incubate
E62 Action:T62 Acts-on:T342
T63 Action 4916 4919 add
E63 Action:T63 Acts-on:T340 Site:T341
T64 Action 5054 5060 remove
E64 Action:T64 Acts-on:T347 Using:T348
T65 Action 5118 5124 Remove
E65 Action:T65 Acts-on:T349 Site:T350
T66 Action 5163 5170 let dry
E66 Action:T66
T67 Action 5238 5247 Resuspend
E67 Action:T67 Acts-on:T353 Using:T355
T68 Action 5292 5295 Mix
E68 Action:T68 Using:T357
T69 Action 5376 5384 Incubate
E69 Action:T69 Acts-on:T358
T70 Action 5438 5443 Place
E70 Action:T70 Acts-on:T361 Site:T362
T71 Action 5524 5532 transfer
E71 Action:T71 Acts-on:T365 Site:T366
T72 Action 5570 5577 Combine
E72 Action:T72 Acts-on:T368 Site:T369
T73 Action 5717 5720 Mix
E73 Action:T73 Using:T375
T74 Action 5740 5745 Apply
E74 Action:T74 Using:T376
T75 Action 5774 5782 incubate
E75 Action:T75 Using:T377
T76 Action 5842 5849 combine
E76 Action:T76 Acts-on:T380 Site:T381
T77 Action 6067 6070 Mix
E77 Action:T77 Using:T390
T78 Action 6090 6095 Apply
E78 Action:T78 Using:T391
T79 Action 6124 6132 incubate
E79 Action:T79 Using:T392
T80 Action 6175 6178 Add
E80 Action:T80 Acts-on:T396 Site:T397
T81 Action 6223 6226 mix
E81 Action:T81 Using:T399
T82 Action 6246 6254 Incubate
E82 Action:T82
T83 Action 6290 6295 Place
E83 Action:T83 Acts-on:T402 Site:T403
T84 Action 6414 6417 Set
E84 Action:T84 Acts-on:T406
T85 Action 6446 6452 remove
E85 Action:T85 Acts-on:T409
T86 Action 6574 6577 add
E86 Action:T86 Acts-on:T414 Site:T415
T87 Action 6762 6765 add
E87 Action:T87 Acts-on:T423 Site:T424
T88 Action 6824 6832 incubate
E88 Action:T88 Acts-on:T425
T89 Action 6636 6644 incubate
E89 Action:T89 Acts-on:T416
T90 Action 6886 6892 remove
E90 Action:T90 Acts-on:T428 Using:T429
T91 Action 6950 6956 Remove
E91 Action:T91 Acts-on:T430 Site:T431
T92 Action 7038 7047 Resuspend
E92 Action:T92 Acts-on:T435 Using:T437
T93 Action 7092 7095 Mix
E93 Action:T93 Using:T439
T94 Action 7181 7189 Incubate
E94 Action:T94 Acts-on:T440
T95 Action 7243 7248 Place
E95 Action:T95 Acts-on:T443 Site:T444
T96 Action 7329 7337 transfer
E96 Action:T96 Acts-on:T448 Site:T449
T97 Action 7373 7376 Add
E97 Action:T97 Acts-on:T451 Site:T452
T98 Action 7421 7424 mix
E98 Action:T98 Using:T454 Acts-on:T452
T99 Action 7444 7452 Incubate
E99 Action:T99
T100 Action 7488 7493 Place
E100 Action:T100 Acts-on:T458 Site:T465
T101 Action 7612 7615 Set
E101 Action:T101 Acts-on:T468
T102 Action 7644 7650 remove
E102 Action:T102 Acts-on:T471
T103 Action 7773 7776 add
E103 Action:T103 Acts-on:T476 Site:T477
T104 Action 7961 7964 add
E104 Action:T104 Acts-on:T488 Site:T489
T105 Action 8023 8031 incubate
E105 Action:T105 Acts-on:T490
T106 Action 7835 7843 incubate
E106 Action:T106 Acts-on:T478
T107 Action 8085 8091 remove
E107 Action:T107 Acts-on:T493 Using:T494
T108 Action 8149 8155 Remove
E108 Action:T108 Acts-on:T497 Site:T498
T109 Action 8194 8201 let dry
E109 Action:T109 Acts-on:T497
T110 Action 8237 8246 Resuspend
E110 Action:T110 Acts-on:T501 Using:T503
T111 Action 8292 8295 Mix
E111 Action:T111 Using:T505
T112 Action 8375 8383 Incubate
E112 Action:T112 Acts-on:T506
T113 Action 8437 8442 Place
E113 Action:T113 Acts-on:T509 Site:T510
T114 Action 8523 8531 transfer
E114 Action:T114 Acts-on:T514 Site:T515
T115 Action 8584 8591 combine
E115 Action:T115 Acts-on:T516 Site:T519
T116 Action 8807 8810 Add
E116 Action:T116 Acts-on:T530 Site:T531
T117 Action 8855 8858 mix
E117 Action:T117 Using:T533
T118 Action 8878 8886 Incubate
E118 Action:T118
T119 Action 8922 8927 Place
E119 Action:T119 Acts-on:T537 Site:T538
T120 Action 9045 9048 Set
E120 Action:T120 Acts-on:T541
T121 Action 9077 9083 remove
E121 Action:T121 Acts-on:T544
T122 Action 9205 9208 add
E122 Action:T122 Acts-on:T549
T123 Action 9267 9275 incubate
E123 Action:T123 Acts-on:T550
T124 Action 9455 9463 incubate
E124 Action:T124 Acts-on:T563
T125 Action 9393 9396 add
E125 Action:T125 Acts-on:T561 Site:T562
T126 Action 9517 9523 remove
E126 Action:T126 Acts-on:T566 Using:T568
T127 Action 9581 9587 Remove
E127 Action:T127 Acts-on:T569 Site:T570
T128 Action 9626 9633 let dry
E128 Action:T128 Acts-on:T569
T129 Action 9669 9678 Resuspend
E129 Action:T129 Acts-on:T573 Using:T575
T130 Action 9723 9726 Mix
E130 Action:T130 Using:T577
T131 Action 9874 9879 Place
E131 Action:T131 Acts-on:T582 Site:T583
T132 Action 9960 9968 transfer
E132 Action:T132 Acts-on:T587 Site:T588
T133 Action 10004 10007 Add
E133 Action:T133 Acts-on:T590 Site:T591
T134 Action 10075 10083 Incubate
E134 Action:T134
T135 Action 10119 10124 Place
E135 Action:T135 Acts-on:T598 Site:T599
T136 Action 10242 10245 Set
E136 Action:T136 Acts-on:T602
T137 Action 10274 10280 remove
E137 Action:T137 Acts-on:T605
T138 Action 10402 10405 add
E138 Action:T138 Acts-on:T610 Site:T611
T139 Action 10464 10472 incubate
E139 Action:T139 Acts-on:T612
T140 Action 10652 10660 incubate
E140 Action:T140 Acts-on:T625
T141 Action 10590 10593 add
E141 Action:T141 Acts-on:T623 Site:T624
T142 Action 10714 10720 remove
E142 Action:T142 Acts-on:T628 Using:T629
T143 Action 10778 10784 Remove
E143 Action:T143 Acts-on:T631 Site:T632
T144 Action 10823 10830 let dry
E144 Action:T144 Acts-on:T631
T145 Action 10866 10875 Resuspend
E145 Action:T145 Acts-on:T635 Using:T637
T146 Action 10920 10923 Mix
E146 Action:T146 Using:T639
T147 Action 11003 11011 Incubate
E147 Action:T147 Acts-on:T640
T148 Action 11065 11070 Place
E148 Action:T148 Acts-on:T643 Site:T644
T149 Action 11151 11159 transfer
E149 Action:T149 Acts-on:T648 Site:T651
T150 Modifier 37 44 Briefly
R1 Mod-Link Arg1:E1 Arg2:T150
T151 Reagent 60 69 component
T152 Temperature 140 143 ice
R2 Setting Arg1:E2 Arg2:T152
T153 Location 160 164 tube
T154 Reagent 185 210 Agencourt AMPure XP Beads
T155 Temperature 222 238 room temperature
R3 Setting Arg1:E4 Arg2:T155
T156 Reagent 254 259 beads
T157 Reagent 339 357 following reagents
T158 Temperature 361 364 ice
R4 Setting Arg1:E6 Arg2:T158
T159 Location 368 403 nuclease-free microcentrifuge tubes
T160 Reagent 408 427 Nuclease-free Water
T161 Reagent 432 435 DNA
T162 Reagent 450 476 NEXTflex™ mtDNA Buffer Mix
T163 Reagent 482 508 NEXTflex™ mtDNA Buffer Mix
T164 Reagent 514 546 NEXTflex™ Nuclear DNA Digest Mix
T165 Amount 509 514 25 μL
R5 Measure Arg1:T164 Arg2:T165
T166 Amount 477 482 15 μL
R6 Measure Arg1:T163 Arg2:T166
T167 Amount 446 450 5 μL
R7 Measure Arg1:T162 Arg2:T167
T168 Amount 437 445 4 – 8 μg
R8 Measure Arg1:T161 Arg2:T168
R9 Meronym Arg1:T160 Arg2:T157
R10 Meronym Arg1:T161 Arg2:T157
R11 Meronym Arg1:T162 Arg2:T157
R12 Meronym Arg1:T163 Arg2:T157
R13 Meronym Arg1:T164 Arg2:T157
T169 Modifier 563 567 well
R14 Mod-Link Arg1:E7 Arg2:T169
T170 Device 596 606 heat block
T171 Time 611 619 48 hours
T172 Temperature 623 627 37°C
R15 Setting Arg1:E8 Arg2:T171
R16 Setting Arg1:E8 Arg2:T172
T173 Reagent 642 648 sample
T174 Temperature 652 656 70°C
T175 Time 661 671 30 minutes
R17 Setting Arg1:E9 Arg2:T174
R18 Setting Arg1:E9 Arg2:T175
T176 Location 682 686 tube
T177 Time 691 701 10 seconds
R19 Setting Arg1:E10 Arg2:T177
T178 Amount 739 744 50 μL
T179 Reagent 748 763 AMPure XP Beads
R20 Measure Arg1:T179 Arg2:T178
T180 Reagent 772 778 sample
T181 Method 571 580 pipetting
T182 Method 795 804 pipetting
T183 Modifier 787 791 well
R21 Mod-Link Arg1:E12 Arg2:T183
T184 Temperature 818 834 room temperature
T185 Time 839 848 5 minutes
R22 Setting Arg1:E13 Arg2:T184
R23 Setting Arg1:E13 Arg2:T185
T186 Location 860 864 tube
T187 Device 872 885 magnetic rack
T188 Temperature 889 905 room temperature
T189 Time 910 919 5 minutes
R24 Setting Arg1:E14 Arg2:T188
R25 Setting Arg1:E14 Arg2:T189
T190 Reagent 984 995 supernatant
T193 Modifier 1093 1099 gently
R26 Mod-Link Arg1:E17 Arg2:T193
T194 Amount 1104 1110 200 μL
T195 Modifier 1114 1130 freshly prepared
T196 Concentration 1131 1134 80%
T197 Reagent 1135 1142 ethanol
R28 Measure Arg1:T197 Arg2:T196
R29 Mod-Link Arg1:T197 Arg2:T195
R30 Measure Arg1:T197 Arg2:T194
T198 Reagent 1151 1171 magnetic bead pellet
T191 Location 1185 1190 plate
T192 Temperature 1194 1210 room temperature
T199 Time 1215 1225 30 seconds
R27 Setting Arg1:E18 Arg2:T192
R31 Setting Arg1:E18 Arg2:T199
T200 Reagent 1245 1252 ethanol
T201 Method 1256 1263 pipette
T202 Modifier 1293 1299 gently
T203 Amount 1304 1310 200 μL
T204 Modifier 1314 1330 freshly prepared
T205 Concentration 1331 1334 80%
T206 Reagent 1335 1342 ethanol
R32 Measure Arg1:T206 Arg2:T205
R33 Mod-Link Arg1:T206 Arg2:T204
R34 Measure Arg1:T206 Arg2:T203
R35 Mod-Link Arg1:E20 Arg2:T202
T207 Reagent 1351 1371 magnetic bead pellet
T208 Location 1385 1390 plate
T209 Temperature 1394 1410 room temperature
T210 Time 1415 1425 30 seconds
R36 Setting Arg1:E21 Arg2:T209
R37 Setting Arg1:E21 Arg2:T210
T211 Reagent 1445 1452 ethanol
T212 Method 1456 1463 pipette
T213 Location 1513 1517 tube
T214 Device 1527 1540 magnetic rack
T215 Temperature 1556 1572 room temperature
T216 Time 1577 1586 3 minutes
R38 Setting Arg1:E24 Arg2:T215
R39 Setting Arg1:E24 Arg2:T216
T217 Reagent 1624 1635 dried beads
T218 Amount 1641 1646 36 μL
T219 Reagent 1647 1666 Nuclease-free Water
R40 Measure Arg1:T219 Arg2:T218
T220 Modifier 1673 1677 well
T221 Method 1681 1690 pipetting
R41 Mod-Link Arg1:E26 Arg2:T220
T222 Reagent 1764 1781 resuspended beads
T223 Temperature 1785 1801 room temperature
T224 Time 1806 1815 2 minutes
R42 Setting Arg1:E27 Arg2:T223
R43 Setting Arg1:E27 Arg2:T224
T225 Location 1823 1827 tube
T226 Device 1831 1844 magnetic rack
T227 Time 1849 1858 5 minutes
R44 Setting Arg1:E28 Arg2:T227
T228 Amount 1910 1915 35 μL
T229 Modifier 1894 1900 Gently
R45 Mod-Link Arg1:E29 Arg2:T229
T230 Reagent 1919 1931 clear sample
R46 Measure Arg1:T230 Arg2:T228
T231 Location 1937 1963 fresh microcentrifuge tube
T232 Method 2033 2042 digestion
T233 Amount 2048 2053 35 μL
T234 Reagent 2057 2072 eluted material
R47 Measure Arg1:T234 Arg2:T233
T235 Reagent 2098 2106 reaction
T236 Amount 2119 2124 35 μL
T237 Reagent 2124 2134 Eluted DNA
T238 Amount 2144 2148 5 μL
T239 Reagent 2148 2174 NEXTflex™ mtDNA Buffer Mix
T240 Amount 2175 2180 15 μL
T241 Reagent 2180 2206 NEXTflex™ mtDNA Buffer Mix
T242 Amount 2207 2212 25 μL
T243 Reagent 2212 2244 NEXTflex™ nuclear DNA Digest Mix
R48 Measure Arg1:T243 Arg2:T242
R49 Measure Arg1:T241 Arg2:T240
R50 Measure Arg1:T239 Arg2:T238
R51 Measure Arg1:T237 Arg2:T236
R52 Meronym Arg1:T237 Arg2:T235
R53 Meronym Arg1:T239 Arg2:T235
R54 Meronym Arg1:T241 Arg2:T235
R55 Meronym Arg1:T243 Arg2:T235
T244 Action 2087 2093 Set up
E150 Action:T244 Creates:T235
T245 Time 2270 2277 2 hours
T246 Temperature 2281 2285 37°C
R56 Setting Arg1:E31 Arg2:T245
R57 Setting Arg1:E31 Arg2:T246
T247 Action 2322 2329 prepare
E151 Action:T247 Creates:T249 Count:T248 Site:T250
T248 Numerical 2330 2333 two
T249 Reagent 2343 2352 reactions
T250 Location 2356 2370 adjacent wells
T251 Location 2384 2393 PCR Plate
R58 Meronym Arg1:T251 Arg2:T250
T252 Temperature 2397 2400 ice
R59 Setting Arg1:E151 Arg2:T252
T253 Modifier 2426 2430 well
T254 Method 2434 2443 pipetting
R60 Mod-Link Arg1:E32 Arg2:T253
T255 Seal 2451 2474 adhesive PCR plate seal
T256 Device 2488 2500 thermocycler
T257 Reagent 2540 2561 pre-stained SYBR Gold
T258 Concentration 2562 2564 2%
R61 Measure Arg1:T257 Arg2:T258
T259 Reagent 2568 2600 Ethidium Bromide TAE agarose gel
R62 Or Arg1:T257 Arg2:T259
T260 Location 2620 2628 gel tray
T261 Amount 2635 2639 3 μL
T262 Reagent 2647 2658 PCR product
R63 Measure Arg1:T262 Arg2:T261
T263 Amount 2670 2674 2 μL
T264 Reagent 2678 2692 6X Loading Dye
R64 Measure Arg1:T264 Arg2:T263
T265 Amount 2697 2701 7 μL
T266 Reagent 2705 2724 Nuclease-free Water
R65 Measure Arg1:T266 Arg2:T265
T267 Reagent 2735 2742 samples
T268 Reagent 2750 2753 gel
T269 Amount 2765 2769 6 μL
T270 Size 2773 2782 MW 100 bp
T271 Reagent 2783 2803 Ready-to-Load Ladder
R66 Measure Arg1:T271 Arg2:T270
R67 Measure Arg1:T271 Arg2:T269
T272 Reagent 2813 2816 gel
T273 Reagent 2822 2835 1X TAE buffer
T274 Numerical 2839 2846 100-120
T275 Measure-Type 2846 2847 V
R68 Of-Type Arg1:T274 Arg2:T275
T276 Time 2852 2866 60-120 minutes
R69 Setting Arg1:E40 Arg2:T276
T277 Reagent 2923 2926 gel
T278 Device 2930 2949 UV transilluminator
T279 Device 2953 2981 gel documentation instrument
R70 Or Arg1:T278 Arg2:T279
T42 Device 3231 3238 pipette
T43 Amount 3242 3248 130 μL
R71 Setting Arg1:E44 Arg2:T43
T280 Modifier 3257 3261 well
T281 Method 3265 3274 pipetting
R72 Mod-Link Arg1:E45 Arg2:T280
T282 Reagent 3289 3295 sample
T283 Location 3301 3313 Covaris tube
T284 Method 3322 3349 manufacturer's instructions
T285 Reagent 3543 3549 sample
T286 Location 3557 3569 Covaris tube
T287 Size 3575 3580 15 mL
T288 Location 3581 3601 microcentrifuge tube
R73 Measure Arg1:T288 Arg2:T287
T289 Reagent 3656 3662 sample
T290 Device 3668 3676 SpeedVac
T291 Time 3681 3688 2 hours
T292 Temperature 3691 3695 45°C
R74 Setting Arg1:E49 Arg2:T291
R75 Setting Arg1:E49 Arg2:T292
T293 Reagent 3961 3978 DNA concentration
T294 Reagent 3911 3932 Qubit® dsDNA reagents
T295 Reagent 4009 4027 following reagents
T296 Temperature 4031 4034 ice
T297 Location 4040 4053;4062 4071 nuclease-free PCR Plate
R76 Setting Arg1:E51 Arg2:T296
T298 Amount 4074 4079 40 μL
T299 Reagent 4079 4084 mtDNA
T300 Amount 4124 4128 7 μL
T301 Reagent 4128 4165 NEXTflex™ mtDNA End Repair Buffer Mix
T302 Amount 4165 4169 3 μL
T303 Reagent 4169 4206 NEXTflex™ mtDNA End Repair Enzyme Mix
R77 Measure Arg1:T303 Arg2:T302
R78 Measure Arg1:T301 Arg2:T300
R79 Measure Arg1:T299 Arg2:T298
R80 Meronym Arg1:T299 Arg2:T295
R81 Meronym Arg1:T301 Arg2:T295
R82 Meronym Arg1:T303 Arg2:T295
T304 Modifier 4223 4227 well
T305 Method 4231 4240 pipetting
R83 Mod-Link Arg1:E52 Arg2:T304
T306 Seal 4248 4271 adhesive PCR plate seal
T307 Action 4276 4284 incubate
E42 Action:T307 Site:T308
T308 Device 4290 4302 thermocycler
T309 Time 4307 4317 30 minutes
T310 Temperature 4321 4325 22°C
R84 Setting Arg1:E42 Arg2:T309
R85 Setting Arg1:E42 Arg2:T310
T311 Amount 4331 4336 80 μL
T312 Reagent 4340 4355 AMPure XP Beads
R86 Measure Arg1:T312 Arg2:T311
T313 Reagent 4364 4370 sample
T314 Modifier 4379 4383 well
T315 Method 4387 4396 pipetting
R87 Mod-Link Arg1:E55 Arg2:T314
T316 Reagent 4407 4413 sample
T317 Temperature 4417 4433 room temperature
T318 Time 4438 4447 5 minutes
R88 Setting Arg1:E56 Arg2:T317
R89 Setting Arg1:E56 Arg2:T318
T319 Location 4467 4476 PCR Plate
T320 Location 4484 4498 magnetic stand
T321 Temperature 4502 4518 room temperature
T322 Time 4523 4532 5 minutes
R90 Setting Arg1:E57 Arg2:T321
R91 Setting Arg1:E57 Arg2:T322
T323 Reagent 4592 4609 clear supernatant
T324 Modifier 4707 4713 gently
T325 Amount 4718 4724 200 μL
T326 Modifier 4728 4744 freshly prepared
T327 Concentration 4745 4748 80%
T328 Reagent 4749 4756 ethanol
R92 Measure Arg1:T328 Arg2:T327
R93 Mod-Link Arg1:T328 Arg2:T326
R94 Measure Arg1:T328 Arg2:T325
R95 Mod-Link Arg1:E60 Arg2:T324
T329 Reagent 4765 4785 magnetic bead pellet
T330 Location 4799 4804 plate
T331 Temperature 4808 4824 room temperature
T332 Time 4829 4839 30 seconds
R96 Setting Arg1:E61 Arg2:T331
R97 Setting Arg1:E61 Arg2:T332
T333 Action 4852 4858 remove
E43 Action:T333 Acts-on:T334 Using:T335
T334 Reagent 4859 4866 ethanol
T335 Method 4870 4877 pipette
T336 Modifier 4909 4915 gently
T337 Amount 4920 4926 200 μL
T338 Modifier 4930 4946 freshly prepared
T339 Concentration 4947 4950 80%
T340 Reagent 4951 4958 ethanol
R98 Measure Arg1:T340 Arg2:T339
R99 Mod-Link Arg1:T340 Arg2:T338
R100 Measure Arg1:T340 Arg2:T337
R101 Mod-Link Arg1:E63 Arg2:T336
T341 Reagent 4967 4987 magnetic bead pellet
T342 Location 5001 5006 plate
T343 Temperature 5010 5026 room temperature
T344 Time 5031 5041 30 seconds
R102 Setting Arg1:E62 Arg2:T343
R103 Setting Arg1:E62 Arg2:T344
T345 Modifier 4841 4850 Carefully
R104 Mod-Link Arg1:E43 Arg2:T345
T346 Modifier 5043 5052 Carefully
R105 Mod-Link Arg1:E64 Arg2:T346
T347 Reagent 5061 5068 ethanol
T348 Method 5072 5079 pipette
T349 Location 5129 5134 plate
T350 Location 5144 5158 magnetic stand
T351 Temperature 5174 5190 room temperature
T352 Time 5195 5204 3 minutes
R106 Setting Arg1:E66 Arg2:T351
R107 Setting Arg1:E66 Arg2:T352
T353 Reagent 5248 5259 dried beads
T354 Amount 5265 5270 17 μL
T355 Reagent 5271 5290 Resuspension Buffer
R108 Measure Arg1:T355 Arg2:T354
T356 Modifier 5296 5300 well
R109 Mod-Link Arg1:E68 Arg2:T356
T357 Method 5304 5313 pipetting
T358 Reagent 5385 5402 resuspended beads
T359 Temperature 5406 5422 room temperature
T360 Time 5427 5436 2 minutes
R110 Setting Arg1:E69 Arg2:T359
R111 Setting Arg1:E69 Arg2:T360
T361 Location 5444 5449 plate
T362 Location 5453 5467 magnetic stand
T363 Time 5472 5481 5 minutes
R112 Setting Arg1:E70 Arg2:T363
T364 Amount 5533 5538 16 μL
T365 Reagent 5542 5554 clear sample
T366 Location 5564 5568 well
T367 Modifier 5517 5523 Gently
R113 Mod-Link Arg1:E71 Arg2:T367
R114 Measure Arg1:T365 Arg2:T364
T368 Reagent 5582 5591 following
T369 Location 5607 5616 PCR Plate
T370 Amount 5618 5623 16 μL
T371 Reagent 5623 5639 End-Repaired DNA
T372 Amount 5665 5671 4.5 μL
T373 Reagent 5671 5702 NEXTflex™ mtDNA Adenylation Mix
R115 Measure Arg1:T373 Arg2:T372
R116 Measure Arg1:T371 Arg2:T370
R117 Meronym Arg1:T371 Arg2:T369
R118 Meronym Arg1:T373 Arg2:T369
T374 Modifier 5721 5725 well
T375 Method 5729 5738 pipetting
R119 Mod-Link Arg1:E73 Arg2:T374
T376 Seal 5746 5769 adhesive PCR plate seal
T377 Device 5788 5800 thermocycler
T378 Time 5805 5815 30 minutes
T379 Temperature 5819 5823 37°C
R120 Setting Arg1:E75 Arg2:T378
R121 Setting Arg1:E75 Arg2:T379
T380 Reagent 5864 5872 reagents
T381 Location 5904 5913 PCR Plate
T382 Reagent 5922 5939 3’ Adenylated DNA
T383 Amount 5915 5922 20.5 μL
T384 Amount 5964 5971 27.5 μL
T385 Reagent 5971 5999 NEXTflex™ mtDNA Ligation Mix
T386 Amount 5999 6005 2.0 μL
T387 Reagent 6005 6028 NEXTflex™ mtDNA Adapter
T388 Reagent 6032 6054 NEXTflex™ ChIP Barcode
R122 Or Arg1:T387 Arg2:T388
R123 Measure Arg1:T387 Arg2:T386
R124 Measure Arg1:T385 Arg2:T384
R125 Measure Arg1:T382 Arg2:T383
R126 Meronym Arg1:T382 Arg2:T380
R127 Meronym Arg1:T385 Arg2:T380
R128 Meronym Arg1:T387 Arg2:T380
T389 Modifier 6071 6075 well
T390 Method 6079 6088 pipetting
R129 Mod-Link Arg1:E77 Arg2:T389
T391 Seal 6096 6119 adhesive PCR plate seal
T392 Device 6138 6150 thermocycler
T393 Time 6155 6165 15 minutes
T394 Temperature 6169 6173 22°C
R130 Setting Arg1:E79 Arg2:T393
R131 Setting Arg1:E79 Arg2:T394
T395 Amount 6179 6184 40 μL
T396 Reagent 6188 6203 AMPure XP Beads
R132 Measure Arg1:T396 Arg2:T395
T397 Reagent 6212 6218 sample
T398 Modifier 6227 6231 well
T399 Method 6235 6244 pipetting
R133 Mod-Link Arg1:E81 Arg2:T398
T400 Temperature 6258 6274 room temperature
T401 Time 6279 6288 5 minutes
R134 Setting Arg1:E82 Arg2:T400
R135 Setting Arg1:E82 Arg2:T401
T402 Location 6308 6317 PCR Plate
T403 Location 6325 6339 magnetic stand
T404 Temperature 6343 6359 room temperature
T405 Time 6364 6373 5 minutes
R136 Setting Arg1:E83 Arg2:T404
R137 Setting Arg1:E83 Arg2:T405
T406 Device 6418 6425 pipette
T407 Amount 6429 6434 88 μL
R138 Setting Arg1:E84 Arg2:T407
T408 Modifier 6439 6445 gently
R139 Mod-Link Arg1:E85 Arg2:T408
T409 Reagent 6453 6470 clear supernatant
T410 Modifier 6567 6573 gently
R140 Mod-Link Arg1:E86 Arg2:T410
T411 Amount 6578 6584 200 μL
T412 Modifier 6588 6604 freshly prepared
T413 Concentration 6605 6608 80%
T414 Reagent 6609 6616 ethanol
R141 Measure Arg1:T414 Arg2:T413
R142 Mod-Link Arg1:T414 Arg2:T412
R143 Measure Arg1:T414 Arg2:T411
T415 Reagent 6625 6631 sample
T416 Location 6645 6650 plate
T417 Temperature 6654 6670 room temperature
T418 Time 6675 6685 30 seconds
R144 Setting Arg1:E89 Arg2:T417
R145 Setting Arg1:E89 Arg2:T418
T419 Modifier 6755 6761 gently
R146 Mod-Link Arg1:E87 Arg2:T419
T420 Amount 6766 6772 200 μL
T421 Modifier 6776 6792 freshly prepared
T422 Concentration 6793 6796 80%
T423 Reagent 6797 6804 ethanol
T424 Reagent 6813 6819 sample
R147 Measure Arg1:T423 Arg2:T422
R148 Mod-Link Arg1:T423 Arg2:T421
R149 Measure Arg1:T423 Arg2:T420
T425 Location 6833 6838 plate
T426 Temperature 6842 6858 room temperature
T427 Time 6863 6873 30 seconds
R150 Setting Arg1:E88 Arg2:T426
R151 Setting Arg1:E88 Arg2:T427
T428 Reagent 6893 6900 ethanol
T429 Device 6904 6911 pipette
T430 Location 6961 6966 plate
T431 Location 6976 6990 magnetic stand
T432 Action 6995 7002 let dry
E152 Action:T432 Acts-on:T430
T433 Temperature 7006 7022 room temperature
T434 Time 7027 7036 3 minutes
R152 Setting Arg1:E152 Arg2:T433
R153 Setting Arg1:E152 Arg2:T434
T435 Reagent 7048 7059 dried beads
T436 Amount 7065 7070 52 μL
T437 Reagent 7071 7090 Resuspension Buffer
R154 Measure Arg1:T437 Arg2:T436
T438 Modifier 7096 7100 well
R155 Mod-Link Arg1:E93 Arg2:T438
T439 Method 7104 7113 pipetting
T440 Reagent 7190 7207 resuspended beads
T441 Temperature 7211 7227 room temperature
T442 Time 7232 7241 2 minutes
R156 Setting Arg1:E94 Arg2:T441
R157 Setting Arg1:E94 Arg2:T442
T443 Location 7249 7254 plate
T444 Location 7258 7272 magnetic stand
T445 Time 7277 7286 5 minutes
R158 Setting Arg1:E95 Arg2:T445
T446 Modifier 7322 7328 Gently
T447 Amount 7338 7343 50 μL
T448 Reagent 7347 7359 clear sample
T449 Location 7363 7371 new well
R159 Measure Arg1:T448 Arg2:T447
R160 Mod-Link Arg1:E96 Arg2:T446
T450 Amount 7377 7382 40 μL
T451 Reagent 7386 7401 AMPure XP Beads
T452 Reagent 7410 7416 sample
R161 Measure Arg1:T451 Arg2:T450
T453 Modifier 7425 7429 well
R162 Mod-Link Arg1:E98 Arg2:T453
T454 Method 7433 7442 pipetting
T455 Temperature 7456 7472 room temperature
T456 Time 7477 7486 5 minutes
R163 Setting Arg1:E99 Arg2:T455
R164 Setting Arg1:E99 Arg2:T456
T457 Size 7498 7505 96 well
T458 Location 7506 7515 PCR Plate
R165 Measure Arg1:T458 Arg2:T457
T459 Size 6300 6307 96 well
R166 Measure Arg1:T402 Arg2:T459
T460 Size 5599 5606 96 well
R167 Measure Arg1:T369 Arg2:T460
T461 Size 4459 4466 96 well
R168 Measure Arg1:T319 Arg2:T461
T462 Size 4054 4061 96 well
R169 Measure Arg1:T297 Arg2:T462
T463 Size 2376 2383 96-well
R170 Measure Arg1:T251 Arg2:T463
T464 Size 5896 5903 96-well
R171 Measure Arg1:T381 Arg2:T464
T465 Location 7523 7537 magnetic stand
T466 Temperature 7541 7557 room temperature
T467 Time 7562 7571 5 minutes
R172 Setting Arg1:E100 Arg2:T466
R173 Setting Arg1:E100 Arg2:T467
T468 Device 7616 7623 pipette
T469 Amount 7627 7632 88 μL
R174 Setting Arg1:E101 Arg2:T469
T470 Modifier 7637 7643 gently
R175 Mod-Link Arg1:E102 Arg2:T470
T471 Reagent 7651 7668 clear supernatant
T472 Modifier 7766 7772 gently
T473 Amount 7777 7783 200 μL
T474 Modifier 7787 7803 freshly prepared
T475 Concentration 7804 7807 80%
T476 Reagent 7808 7815 ethanol
R176 Measure Arg1:T476 Arg2:T475
R177 Mod-Link Arg1:T476 Arg2:T474
R178 Measure Arg1:T476 Arg2:T473
R179 Mod-Link Arg1:E103 Arg2:T472
T477 Reagent 7824 7830 sample
T478 Location 7844 7849 plate
T479 Temperature 7853 7869 room temperature
T480 Time 7874 7884 30 seconds
R180 Setting Arg1:E106 Arg2:T479
R181 Setting Arg1:E106 Arg2:T480
T481 Action 7897 7903 remove
E153 Action:T481 Acts-on:T482 Using:T483
T482 Reagent 7904 7911 ethanol
T483 Device 7915 7922 pipette
T484 Modifier 7954 7960 gently
T485 Amount 7965 7971 200 μL
T486 Modifier 7975 7991 freshly prepared
T487 Concentration 7992 7995 80%
T488 Reagent 7996 8003 ethanol
T489 Reagent 8012 8018 sample
R182 Measure Arg1:T488 Arg2:T487
R183 Mod-Link Arg1:T488 Arg2:T486
R184 Measure Arg1:T488 Arg2:T485
R185 Mod-Link Arg1:E104 Arg2:T484
T490 Location 8032 8037 plate
T491 Temperature 8041 8057 room temperature
T492 Time 8062 8072 30 seconds
R186 Setting Arg1:E105 Arg2:T491
R187 Setting Arg1:E105 Arg2:T492
T493 Reagent 8092 8099 ethanol
T494 Device 8103 8110 pipette
T495 Modifier 7886 7895 Carefully
T496 Modifier 8074 8083 Carefully
R188 Mod-Link Arg1:E153 Arg2:T495
R189 Mod-Link Arg1:E107 Arg2:T496
T497 Location 8160 8165 plate
T498 Location 8175 8189 magnetic stand
T499 Temperature 8205 8221 room temperature
T500 Time 8226 8235 3 minutes
R190 Setting Arg1:E109 Arg2:T499
R191 Setting Arg1:E109 Arg2:T500
T501 Reagent 8247 8258 dried beads
T502 Amount 8264 8269 38 μL
T503 Reagent 8270 8289 Resuspension Buffer
R192 Measure Arg1:T503 Arg2:T502
T504 Modifier 8296 8300 well
T505 Method 8304 8313 pipetting
R193 Mod-Link Arg1:E111 Arg2:T504
T506 Reagent 8384 8401 resuspended beads
T507 Temperature 8405 8421 room temperature
T508 Time 8426 8435 2 minutes
R194 Setting Arg1:E112 Arg2:T507
R195 Setting Arg1:E112 Arg2:T508
T509 Location 8443 8448 plate
T510 Location 8452 8466 magnetic stand
T511 Time 8471 8480 5 minutes
R196 Setting Arg1:E113 Arg2:T511
T512 Modifier 8516 8522 Gently
T513 Amount 8532 8537 36 μL
T514 Reagent 8541 8553 clear sample
T515 Location 8557 8565 new well
R197 Measure Arg1:T514 Arg2:T513
R198 Mod-Link Arg1:E114 Arg2:T512
T516 Reagent 8596 8614 following reagents
T517 Temperature 8618 8621 ice
T518 Size 8629 8636 96 well
T519 Location 8637 8646 PCR plate
R199 Measure Arg1:T519 Arg2:T518
R200 Setting Arg1:E115 Arg2:T517
T520 Amount 8648 8653 36 μL
T521 Reagent 8653 8678 Purified Ligation Product
T522 Amount 8706 8711 12 μL
T523 Reagent 8711 8735 NEXTflex™ PCR Master Mix
T524 Amount 8735 8739 2 μL
T525 Reagent 8739 8759 NEXTflex™ Primer Mix
R201 Measure Arg1:T525 Arg2:T524
R202 Measure Arg1:T523 Arg2:T522
R203 Measure Arg1:T521 Arg2:T520
R204 Meronym Arg1:T521 Arg2:T516
R205 Meronym Arg1:T523 Arg2:T516
R206 Meronym Arg1:T525 Arg2:T516
T526 Action 8772 8775 Mix
E154 Action:T526 Using:T528
T527 Modifier 8776 8780 well
T528 Method 8784 8793 pipetting
R207 Mod-Link Arg1:E154 Arg2:T527
T529 Amount 8811 8816 40 μL
T530 Reagent 8820 8835 AMPure XP Beads
R208 Measure Arg1:T530 Arg2:T529
T531 Reagent 8844 8850 sample
T532 Modifier 8859 8863 well
T533 Method 8867 8876 pipetting
R209 Mod-Link Arg1:E117 Arg2:T532
T534 Temperature 8890 8906 room temperature
T535 Time 8911 8920 5 minutes
R210 Setting Arg1:E118 Arg2:T534
R211 Setting Arg1:E118 Arg2:T535
T536 Size 8932 8939 96 well
T537 Location 8940 8949 PCR Plate
T538 Location 8957 8971 magnetic stand
R212 Measure Arg1:T537 Arg2:T536
T539 Temperature 8975 8991 room temperature
T540 Time 8996 9005 5 minutes
R213 Setting Arg1:E119 Arg2:T539
R214 Setting Arg1:E119 Arg2:T540
T541 Device 9049 9056 pipette
T542 Amount 9060 9065 88 μL
R215 Setting Arg1:E120 Arg2:T542
T543 Modifier 9070 9076 gently
R216 Mod-Link Arg1:E121 Arg2:T543
T544 Reagent 9084 9101 clear supernatant
T545 Modifier 9198 9204 gently
T546 Amount 9209 9215 200 μL
T547 Modifier 9220 9235 reshly prepared
T548 Concentration 9236 9239 80%
T549 Reagent 9240 9247 ethanol
R217 Measure Arg1:T549 Arg2:T548
R218 Mod-Link Arg1:T549 Arg2:T547
R219 Measure Arg1:T549 Arg2:T546
T550 Location 9276 9281 plate
T551 Temperature 9285 9301 room temperature
T552 Time 9306 9316 30 seconds
R220 Setting Arg1:E123 Arg2:T551
R221 Setting Arg1:E123 Arg2:T552
T553 Action 9329 9335 remove
E155 Action:T553 Acts-on:T554 Using:T555
T554 Reagent 9336 9343 ethanol
T555 Device 9347 9354 pipette
T556 Modifier 9318 9327 Carefully
R222 Mod-Link Arg1:E155 Arg2:T556
T557 Modifier 9386 9392 gently
T558 Amount 9397 9403 200 μL
T559 Modifier 9407 9423 freshly prepared
T560 Concentration 9424 9427 80%
T561 Reagent 9428 9435 ethanol
T562 Reagent 9444 9450 sample
R223 Measure Arg1:T561 Arg2:T560
R224 Mod-Link Arg1:T561 Arg2:T559
R225 Measure Arg1:T561 Arg2:T558
R226 Mod-Link Arg1:E125 Arg2:T557
T563 Location 9464 9469 plate
T564 Temperature 9473 9489 room temperature
T565 Time 9494 9504 30 seconds
R227 Setting Arg1:E124 Arg2:T564
R228 Setting Arg1:E124 Arg2:T565
T566 Reagent 9524 9531 ethanol
T567 Modifier 9506 9515 Carefully
T568 Device 9535 9542 pipette
R229 Mod-Link Arg1:E126 Arg2:T567
T569 Location 9592 9597 plate
T570 Location 9607 9621 magnetic stand
T571 Temperature 9637 9653 room temperature
T572 Time 9658 9667 3 minutes
R230 Setting Arg1:E128 Arg2:T571
R231 Setting Arg1:E128 Arg2:T572
T573 Reagent 9679 9690 dried beads
T574 Amount 9696 9701 52 μL
T575 Reagent 9702 9721 Resuspension Buffer
R232 Measure Arg1:T575 Arg2:T574
T576 Modifier 9727 9731 well
T577 Method 9735 9744 pipetting
R233 Mod-Link Arg1:E130 Arg2:T576
T578 Action 9812 9820 Incubate
E156 Action:T578 Acts-on:T579
T579 Reagent 9821 9838 resuspended beads
T580 Temperature 9842 9858 room temperature
T581 Time 9863 9872 2 minutes
R234 Setting Arg1:E156 Arg2:T580
R235 Setting Arg1:E156 Arg2:T581
T582 Location 9880 9885 plate
T583 Location 9889 9903 magnetic stand
T584 Time 9908 9917 5 minutes
R236 Setting Arg1:E131 Arg2:T584
T585 Modifier 9953 9959 Gently
T586 Amount 9969 9974 50 μL
T587 Reagent 9978 9990 clear sample
T588 Location 9994 10002 new well
R237 Mod-Link Arg1:E132 Arg2:T585
R238 Measure Arg1:T587 Arg2:T586
T589 Amount 10008 10013 40 μL
T590 Reagent 10017 10032 AMPure XP Beads
T591 Reagent 10041 10047 sample
T592 Action 10052 10055 mix
E157 Action:T592 Using:T593
T593 Method 10064 10073 pipetting
T594 Modifier 10056 10060 well
R239 Measure Arg1:T590 Arg2:T589
R240 Mod-Link Arg1:E157 Arg2:T594
T595 Temperature 10087 10103 room temperature
T596 Time 10108 10117 5 minutes
R241 Setting Arg1:E134 Arg2:T595
R242 Setting Arg1:E134 Arg2:T596
T597 Size 10129 10136 96 well
T598 Location 10137 10146 PCR Plate
R243 Measure Arg1:T598 Arg2:T597
T599 Location 10154 10168 magnetic stand
T600 Temperature 10172 10188 room temperature