-
Notifications
You must be signed in to change notification settings - Fork 3
/
Copy pathprotocol_515.ann
155 lines (155 loc) · 4.54 KB
/
protocol_515.ann
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
T1 Action 98 101 mix
E1 Action:T1 Acts-on:T26 Acts-on2:T29 Acts-on3:T34
T2 Action 183 186 Add
E2 Action:T2 Acts-on:T37 Site:T38
T3 Action 226 230 Heat
E3 Action:T3 Acts-on:T39
T4 Action 261 266 Layer
E4 Action:T4 Acts-on:T42 Using:T44 Site:T45
T5 Action 378 381 Add
E5 Action:T5 Acts-on:T47
T6 Action 428 438 Centrifuge
E6 Action:T6 Acts-on:T48 Using:T49
T7 Action 499 505 Remove
E7 Action:T7 Acts-on:T53 Using:T54 Site:T55
T9 Action 640 646 adding
E9 Action:T9 Acts-on:T57 Site:T58
T10 Action 717 723 mixing
E10 Action:T10 Using:T60
T11 Action 745 755 centrifuge
E11 Action:T11 Using:T63
T12 Action 822 831 pipet off
E12 Action:T12 Acts-on:T64
T8 Action 879 882 Add
E8 Action:T8 Acts-on:T68 Site:T69
T13 Action 920 926 adjust
E13 Action:T13 Using:T70
T14 Action 981 992 Precipitate
E14 Action:T14 Acts-on:T71 Using:T74
T15 Action 1032 1035 Mix
E15 Action:T15
T16 Action 1045 1049 hold
E16 Action:T16
T17 Action 1070 1080 Centrifuge
E17 Action:T17 Acts-on:T79 Using:T80
T18 Action 1151 1158 Discard
E18 Action:T18 Acts-on:T84
T19 Action 1177 1180 Dry
E19 Action:T19 Acts-on:T85 Using:T87
T20 Action 1258 1267 Resuspend
E20 Action:T20 Acts-on:T88 Using:T91
T21 Action 1327 1336 Determine
E21 Action:T21
T22 Action 1364 1369 store
E22 Action:T22 Acts-on:T94
T23 Size 79 90 100 x 13 mm
T24 Location 91 96 tubes
R1 Measure Arg1:T24 Arg2:T23
T25 Amount 111 117 500 µL
T26 Reagent 121 126 virus
R2 Measure Arg1:T26 Arg2:T25
T27 Amount 128 133 60 µL
T28 Concentration 137 140 10X
T29 Reagent 141 144 TEN
R3 Measure Arg1:T29 Arg2:T28
R4 Measure Arg1:T29 Arg2:T27
T30 Measure-Type 146 148 pH
T31 Numerical 149 152 7.4
R5 Of-Type Arg1:T31 Arg2:T30
T32 Amount 158 163 60 µL
T33 Concentration 167 169 1%
T34 Reagent 170 181 Na sarcosyl
R6 Measure Arg1:T34 Arg2:T33
R7 Measure Arg1:T34 Arg2:T32
T35 Amount 187 193 600 µL
T36 Concentration 197 200 60%
T37 Reagent 207 211 CsCl
T38 Location 220 224 tube
R8 Measure Arg1:T37 Arg2:T36
R9 Measure Arg1:T37 Arg2:T35
T39 Location 235 240 tubes
T40 Temperature 244 248 75°C
T41 Time 253 259 15 min
R10 Setting Arg1:E3 Arg2:T40
R11 Setting Arg1:E3 Arg2:T41
T42 Reagent 271 278 samples
T43 Concentration 295 301 40-60%
T44 Reagent 308 312 CsCl
R12 Measure Arg1:T44 Arg2:T43
T45 Location 326 342 SW60 rotor tubes
T46 Amount 382 389 1200 µL
T47 Reagent 397 409 heated virus
R13 Measure Arg1:T47 Arg2:T46
T48 Reagent 443 452 gradients
T49 Device 456 467 SW60 rotors
T50 Speed 471 481 35,000 rpm
T51 Time 483 491 18 hours
T52 Temperature 493 497 25°C
R14 Setting Arg1:E6 Arg2:T50
R15 Setting Arg1:E6 Arg2:T51
R16 Setting Arg1:E6 Arg2:T52
T53 Reagent 510 519 DNA bands
T54 Device 546 562 wide bent needle
T55 Location 566 580;587 598 silicon-coated corex tubes
T56 Size 581 586 30 mL
R17 Measure Arg1:T55 Arg2:T56
T57 Reagent 666 695 CsCl/TE-saturated isopropanol
T58 Reagent 703 715 DNA solution
T59 Modifier 724 730 gently
T60 Method 734 743 inversion
R18 Mod-Link Arg1:E10 Arg2:T59
T61 Time 760 765 1 min
T62 Speed 769 778 3,000 rpm
T63 Device 786 793 Sorvall
T64 Reagent 842 859 isopropanol layer
R19 Setting Arg1:E11 Arg2:T61
R20 Setting Arg1:E11 Arg2:T62
T65 Generic-Measure 650 662 equal volume
R21 Measure Arg1:T57 Arg2:T65
T66 Amount 883 889 1.0 mL
T67 Concentration 893 896 3 M
T68 Reagent 897 902 NaOAc
R22 Measure Arg1:T68 Arg2:T67
R23 Measure Arg1:T68 Arg2:T66
T69 Location 911 915 tube
T70 Reagent 970 979 TE buffer
T72 Amount 954 961 10.0 mL
R24 Setting Arg1:E13 Arg2:T72
T71 Reagent 997 1001 DNAs
T73 Concentration 1021 1025 100%
T74 Reagent 1026 1030 EtOH
R25 Measure Arg1:T74 Arg2:T73
T75 Generic-Measure 1007 1017 2X volumes
R26 Measure Arg1:T74 Arg2:T75
T76 Modifier 1036 1040 well
T77 Temperature 1053 1058 -20°C
T78 Time 1059 1068 overnight
R27 Mod-Link Arg1:E15 Arg2:T76
R28 Setting Arg1:E16 Arg2:T77
R29 Setting Arg1:E16 Arg2:T78
T79 Reagent 1085 1096 DNA samples
T80 Device 1104 1122 Sorvall HB-4 rotor
T81 Speed 1126 1136 10,000 rpm
T82 Time 1138 1144 20 min
T83 Temperature 1146 1149 4°C
R30 Setting Arg1:E17 Arg2:T81
R31 Setting Arg1:E17 Arg2:T82
R32 Setting Arg1:E17 Arg2:T83
T84 Reagent 1163 1175 supernatants
T85 Reagent 1185 1192 pellets
T86 Time 1202 1211 10-15 min
T87 Device 1220 1237 vacuum desiccator
R33 Setting Arg1:E19 Arg2:T86
T88 Reagent 1272 1283 DNA samples
T89 Amount 1303 1309 500 µL
T90 Concentration 1313 1315 1X
T91 Reagent 1316 1325 TE buffer
T92 Concentration 967 969 1X
R34 Measure Arg1:T70 Arg2:T92
R35 Measure Arg1:T91 Arg2:T90
R36 Measure Arg1:T91 Arg2:T89
T93 Generic-Measure 1341 1359 DNA concentrations
R37 Setting Arg1:E21 Arg2:T93
T94 Reagent 1374 1378 DNAs
T95 Temperature 1382 1385 4°C
R38 Setting Arg1:E22 Arg2:T95