protocol_527.ann

T1	Action 64 69	place
E1	Action:T1 Acts-on:T16 Site:T17
T2	Action 127 130	Add
E2	Action:T2 Acts-on:T20
T3	Action 154 159	rinse
E3	Action:T3 
T4	Action 179 186	Discard
E4	Action:T4 Acts-on:T22
T5	Action 216 219	add
E5	Action:T5 Acts-on:T24
T6	Action 282 285	Add
E6	Action:T6 Acts-on:T26
T7	Action 311 316	rinse
E7	Action:T7 
T8	Action 333 340	Discard
E8	Action:T8 Acts-on:T28
T9	Action 354 357	add
E9	Action:T9 Acts-on:T30
T10	Action 386 391	Place
E10	Action:T10 Acts-on:T31 Site:T32
T11	Action 460 465	Rinse
E11	Action:T11 Acts-on:T33 Using:T34
T12	Action 618 623	rinse
E12	Action:T12 Acts-on:T35 Using:T36
T13	Action 678 682	Wash
E13	Action:T13 Acts-on:T37 Using:T41
T14	Action 796 801	rinse
E14	Action:T14 Using:T42
T15	Action 818 823	store
E15	Action:T15 Acts-on:T44 Site:T43
T16	Device 74 82	membrane
T17	Location 97 101	tray
T18	Amount 131 135	20ml
T19	Concentration 136 140	100%
T20	Reagent 141 149	methanol
R1	Measure Arg1:T20 Arg2:T19	
R2	Measure Arg1:T20 Arg2:T18	
T21	Time 164 177	10‐30 seconds
R3	Setting Arg1:E3 Arg2:T21	
T22	Reagent 191 199	methanol
T23	Amount 220 224	12ml
T24	Reagent 225 246	Swift™ Membrane Stain
R4	Measure Arg1:T24 Arg2:T23	
T25	Amount 286 290	20ml
T26	Reagent 291 306	deionized water
R5	Measure Arg1:T26 Arg2:T25	
T27	Time 321 331	30 seconds
R6	Setting Arg1:E7 Arg2:T27	
T28	Reagent 345 349	wash
T29	Amount 358 362	12ml
T30	Reagent 363 384	Swift™ Membrane Stain
R7	Measure Arg1:T30 Arg2:T29	
T31	Location 396 401	trays
T32	Device 407 421	rocking shaker
T33	Device 470 478	membrane
T34	Reagent 482 490	DI water
T35	Device 628 636	membrane
T36	Reagent 640 648	DI water
T37	Reagent 687 696	membranes
T38	Time 701 714	30‐60 seconds
R8	Setting Arg1:E13 Arg2:T38	
T39	Amount 718 722	50ml
T40	Concentration 723 725	1X
T41	Reagent 726 740	Swift™ Destain
R9	Measure Arg1:T41 Arg2:T40	
R10	Measure Arg1:T41 Arg2:T39	
T42	Reagent 805 813	DI water
T43	Reagent 836 844	DI water
T44	Reagent 828 832	blot