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protocol_547.ann
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T1 Action 52 56 Pull
E1 Action:T1 Acts-on:T36
T2 Action 124 131 Perform
E2 Action:T2 Using:T39 Site:T41
T3 Action 222 227 Store
E3 Action:T3 Acts-on:T42
T4 Action 256 262 Absorb
E4 Action:T4 Acts-on:T44 Site:T45
T5 Action 94 98 grow
E5 Action:T5 Site:T38 Acts-on:T36
T6 Action 293 298 Place
E6 Action:T6 Acts-on:T47 Acts-on2:T49 Site:T50
T7 Action 406 411;427 433 Allow absorb
E7 Action:T7 Acts-on:T53 Site:T54
T8 Action 463 466 Add
E8 Action:T8 Acts-on:T57 Site:T58
T9 Action 521 525 Seal
E9 Action:T9 Acts-on:T59 Using:T60
T10 Action 554 562 incubate
E10 Action:T10 Using:T61
T13 Action 680 692 transferring
E13 Action:T13 Site:T12 Site2:T63
T14 Action 833 843 filter out
E14 Action:T14 Acts-on:T65 Using:T71 Site:T68
T15 Action 899 903 Spin
E15 Action:T15
T16 Action 941 949 Transfer
E16 Action:T16 Acts-on:T72 Site:T73
T17 Action 991 996 Store
E17 Action:T17
T18 Action 1047 1054 growing
E18 Action:T18 Acts-on:T75 Acts-on2:T76 Site:T77
T19 Action 1091 1098 Prepare
E19 Action:T19 Creates:T78 Creates2:T80
T20 Action 1140 1146 Absorb
E20 Action:T20 Acts-on:T84 Site:T86 Site2:T88
T21 Action 1277 1280 Mix
E21 Action:T21 Acts-on:T81 Site:T90
T23 Action 1361 1369 Incubate
E23 Action:T23
T24 Action 1374 1378 look
E24 Action:T24 Acts-on:T92
T25 Action 1402 1406 Pick
E25 Action:T25 Acts-on:T93
T26 Action 1455 1461 record
E26 Action:T26 Acts-on:T96
T27 Action 1490 1495 Store
E27 Action:T27 Acts-on:T97
T28 Action 1567 1578 diffuse out
E28 Action:T28 Acts-on:T100 Site:T101 Site2:T102
T29 Action 1713 1717 Take
E29 Action:T29 Acts-on:T104
T30 Action 1786 1792 absorb
E30 Action:T30 Acts-on:T104 Acts-on2:T106
T31 Action 1833 1836 Add
E31 Action:T31 Acts-on:T109
T32 Action 1855 1859 grow
E32 Action:T32 Using:T110
T33 Action 1875 1885 Centrifuge
E33 Action:T33 Acts-on:T111
T34 Action 1900 1906 filter
E34 Action:T34 Acts-on:T112 Using:T114
T35 Action 2016 2022 Purify
E35 Action:T35 Acts-on:T118 Using:T119
T36 Reagent 57 77 Roseobacter cultures
T37 Amount 102 106 5 ml
T38 Reagent 107 121 broth cultures
R1 Measure Arg1:T38 Arg2:T37
T39 Method 132 147 virus screening
T40 Size 159 166 96 well
T41 Location 167 219 flat-bottom tissue culture treated microtiter plates
R2 Measure Arg1:T41 Arg2:T40
T42 Reagent 228 246 seawater filtrates
T43 Temperature 250 253 4°C
R3 Setting Arg1:E3 Arg2:T43
T44 Reagent 267 274 viruses
T45 Reagent 282 290 bacteria
T46 Amount 299 304 15 µl
T47 Reagent 308 323 log phase cells
T48 Amount 328 333 15 µl
T49 Reagent 337 354 seawater filtrate
R4 Measure Arg1:T47 Arg2:T46
R5 Measure Arg1:T49 Arg2:T48
T50 Location 379 384 wells
T51 Size 390 397 96 well
T52 Location 398 403 plate
R6 Measure Arg1:T52 Arg2:T51
R7 Meronym Arg1:T50 Arg2:T52
T53 Reagent 416 423 viruses
T54 Reagent 441 449 bacteria
T55 Time 454 460 1 hour
R8 Setting Arg1:E7 Arg2:T55
T56 Amount 467 473 200 µl
T57 Reagent 474 486 growth media
R9 Measure Arg1:T57 Arg2:T56
T58 Location 513 518 wells
T59 Location 530 535 plate
T60 Reagent 541 549 parafilm
T61 Method 568 575 shaking
T11 Size 706 713 96 well
T12 Location 714 719 plate
R10 Measure Arg1:T12 Arg2:T11
T62 Size 731 738 96-well
T63 Location 739 744 plate
R11 Measure Arg1:T63 Arg2:T62
T64 Reagent 756 774 fresh liquid media
R12 Meronym Arg1:T64 Arg2:T63
T65 Reagent 844 849 cells
T66 Amount 855 860 0.2um
T67 Size 877 882 1.5ml
T68 Location 883 898 microfuge tubes
R13 Measure Arg1:T68 Arg2:T67
T69 Time 908 914 5 mins
R14 Setting Arg1:E15 Arg2:T69
T70 Speed 919 922 max
R15 Setting Arg1:E15 Arg2:T70
T71 Device 861 871 filtration
R16 Measure Arg1:T71 Arg2:T66
T72 Reagent 950 961 supernatant
T73 Location 965 975 fresh tube
T74 Temperature 1000 1003 4°C
R17 Setting Arg1:E17 Arg2:T74
T75 Reagent 1055 1060 cells
T76 Reagent 1066 1071 virus
T77 Reagent 1075 1086 solid media
T78 Reagent 1099 1115 solid agar media
T79 Concentration 1120 1124 0.5%
T80 Reagent 1125 1137 overlay agar
R18 Measure Arg1:T80 Arg2:T79
T83 Amount 1189 1194 25 µl
T84 Reagent 1195 1200 virus
T85 Size 1206 1213 96 well
T86 Location 1214 1219 plate
T87 Amount 1236 1242 200 µl
T88 Reagent 1243 1266 log-phase host bacteria
T89 Time 1271 1274 1hr
R19 Measure Arg1:T84 Arg2:T83
R20 Measure Arg1:T86 Arg2:T85
R21 Measure Arg1:T88 Arg2:T87
R22 Setting Arg1:E20 Arg2:T89
T81 Reagent 1285 1305 virus-absorbed cells
T82 Amount 1313 1317 5 ml
T90 Reagent 1318 1330 overlay agar
R23 Measure Arg1:T90 Arg2:T82
T22 Action 1333 1342 Pour over
E11 Action:T22 Acts-on:T91
T91 Reagent 1347 1358 solid media
T92 Reagent 1383 1389 plaque
T93 Reagent 1407 1422 representatives
T94 Reagent 1430 1442 plaque types
R24 Meronym Arg1:T93 Arg2:T94
T95 Generic-Measure 1462 1472 appearance
T96 Reagent 1480 1487 plaques
R25 Measure Arg1:T96 Arg2:T95
T97 Reagent 1500 1505 broth
T98 Reagent 1524 1534 agar plugs
R26 Meronym Arg1:T98 Arg2:T97
T99 Temperature 1538 1541 4°C
R27 Setting Arg1:E27 Arg2:T99
T100 Reagent 1556 1563 viruses
T101 Reagent 1586 1590 agar
T102 Reagent 1600 1605 broth
T103 Amount 1718 1723 25 µl
T104 Reagent 1731 1752 plaque-purified virus
R28 Measure Arg1:T104 Arg2:T103
T105 Amount 1796 1802 200 µl
T106 Reagent 1803 1821 log-phase bacteria
R29 Measure Arg1:T106 Arg2:T105
T107 Time 1826 1830 1 hr
R30 Setting Arg1:E30 Arg2:T107
T108 Amount 1840 1844 5 ml
T109 Reagent 1845 1850 broth
R31 Measure Arg1:T109 Arg2:T108
T110 Method 1865 1872 shaking
T111 Reagent 1890 1895 cells
T112 Reagent 1907 1914 culture
T113 Size 1923 1929 0.2 µm
T114 Device 1930 1936 filter
R32 Measure Arg1:T114 Arg2:T113
T115 Action 1937 1947 SYBR stain
E12 Action:T115 Acts-on:T116
T116 Reagent 1950 1957 portion
T117 Reagent 1965 1973 filtrate
R33 Meronym Arg1:T116 Arg2:T117
T118 Reagent 2023 2026 DNA
T119 Method 2037 2066 Promega Wizard Lambda DNA kit