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protocol_585.ann
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T1 Action 146 154 Transfer
E1 Action:T1 Site:T14
T2 Action 238 241 Add
E2 Action:T2 Acts-on:T23 Site:T19
T3 Action 346 354 Incubate
E3 Action:T3 Acts-on:T20
T4 Action 694 700 Insert
E4 Action:T4 Acts-on:T25 Site:T26
T5 Action 762 766 Spin
E5 Action:T5
T6 Action 861 864 Add
E6 Action:T6 Acts-on:T38
T8 Action 1046 1049 Add
E8 Action:T8 Acts-on:T46
T9 Action 1077 1081 spin
E9 Action:T9
T10 Action 1149 1157 Transfer
E10 Action:T10 Acts-on:T49 Site:T52
T11 Action 1330 1333 Add
E11 Action:T11 Acts-on:T54 Acts-on2:T55
T12 Action 1392 1396 Wait
E12 Action:T12
T13 Action 1415 1419 spin
E13 Action:T13
T14 Location 167 181 microfuge tube
T15 Reagent 196 205 gel slice
T16 Action 186 191 weigh
E14 Action:T16 Acts-on:T15
T17 Amount 160 166 1.5 ml
R1 Measure Arg1:T14 Arg2:T17
T18 Reagent 255 276 Gel Dissolving Buffer
T19 Reagent 284 293 gel slice
T20 Reagent 359 365 sample
T21 Temperature 393 397 50°C
T22 Time 455 467 5–10 minutes
R2 Setting Arg1:E3 Arg2:T21
R3 Setting Arg1:E3 Arg2:T22
T23 Reagent 244 251 volumes
R4 Meronym Arg1:T23 Arg2:T18
T24 Numerical 242 243 4
R5 Measure Arg1:T23 Arg2:T24
T25 Location 701 707 column
T26 Location 713 728 collection tube
T27 Action 733 737 load
E15 Action:T27 Acts-on:T28 Site:T29
T28 Reagent 738 744 sample
T29 Location 754 760 column
T30 Time 771 779 1 minute
T31 Speed 783 793 16,000 x g
T32 Action 800 807 discard
E16 Action:T32 Acts-on:T33
T33 Reagent 808 820 flow-through
R6 Setting Arg1:E5 Arg2:T30
R7 Setting Arg1:E5 Arg2:T31
T34 Action 822 831 Re-insert
E17 Action:T34 Acts-on:T35 Site:T36
T35 Location 832 838 column
T36 Location 844 859 collection tube
T37 Amount 865 871 200 μl
T38 Reagent 872 887 DNA Wash Buffer
T39 Reagent 894 901 ethanol
R8 Meronym Arg1:T39 Arg2:T38
T40 Action 913 917 spin
E18 Action:T40
T41 Time 922 930 1 minute
T42 Speed 934 944 16,000 x g
R9 Setting Arg1:E18 Arg2:T41
R10 Setting Arg1:E18 Arg2:T42
R11 Measure Arg1:T38 Arg2:T37
T7 Action 1007 1016 Re-insert
E7 Action:T7 Acts-on:T43 Site:T44
T43 Location 1017 1023 column
T44 Location 1029 1044 collection tube
T45 Amount 1050 1056 200 μl
T46 Reagent 1057 1072 DNA Wash Buffer
R12 Measure Arg1:T46 Arg2:T45
T47 Time 1086 1094 1 minute
T48 Speed 1098 1108 16,000 x g
R13 Setting Arg1:E9 Arg2:T47
R14 Setting Arg1:E9 Arg2:T48
T49 Location 1158 1164 column
T50 Modifier 1170 1175 clean
T51 Amount 1176 1182 1.5 ml
T52 Location 1183 1197 microfuge tube
R15 Measure Arg1:T52 Arg2:T51
R16 Mod-Link Arg1:T52 Arg2:T50
T53 Amount 1334 1340 ≥ 6 μl
T54 Reagent 1344 1362 DNA Elution Buffer
R17 Measure Arg1:T54 Arg2:T53
T55 Location 1370 1376 center
T56 Location 1384 1390 matrix
R18 Meronym Arg1:T55 Arg2:T56
T57 Time 1401 1409 1 minute
T58 Time 1424 1432 1 minute
T59 Speed 1436 1446 16,000 x g
T60 Action 1450 1455 elute
E19 Action:T60 Acts-on:T61 Using:E12 Using2:E13
T61 Reagent 1460 1463 DNA
R19 Setting Arg1:E13 Arg2:T58
R20 Setting Arg1:E13 Arg2:T59
R21 Setting Arg1:E12 Arg2:T57