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protocol_70.ann
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T1 Action 68 75 Pipette
E1 Action:T1 Acts-on:T29 Site:T27
T3 Action 151 156 Float
E3 Action:T3 Acts-on:T33 Using:T38
T4 Action 307 314 balance
E4 Action:T4 Acts-on:T33
T5 Action 335 339 Load
E5 Action:T5 Acts-on:T34 Using:T35
T6 Action 390 394 spin
E6 Action:T6 Acts-on:T34
T7 Action 421 428 Recover
E7 Action:T7 Acts-on:T30
T8 Action 456 462 Remove
E8 Action:T8 Acts-on:T31
T9 Action 483 492 resuspend
E9 Action:T9 Acts-on:T32
T10 Action 505 512 Perform
E10 Action:T10 Site:T42 Acts-on:T65
T11 Action 592 596 Thaw
E11 Action:T11 Acts-on:T46
T12 Action 620 623 add
E12 Action:T12 Acts-on:T48 Site:T50
T13 Action 671 679 incubate
E13 Action:T13 Acts-on:T50 Site:T67
T14 Action 704 710 Verify
E14 Action:T14 Acts-on:T53
T15 Action 762 771 pipetting
E15 Action:T15 Acts-on:T54
T16 Action 820 823 Add
E16 Action:T16 Acts-on:T59
T17 Action 852 859 observe
E17 Action:T17 Acts-on:T60 Site:T61
T18 Action 908 911 Add
E18 Action:T18 Acts-on:T62 Using:T64
T19 Action 962 968 respin
E19 Action:T19 Acts-on:T64
T20 Action 979 985 Remove
E20 Action:T20 Acts-on:T45
T21 Action 1006 1015 resuspend
E21 Action:T21 Acts-on:T45
T22 Amount 76 82 1.7 mL
T23 Size 93 100 0.22-µm
T25 Action 1062 1068 Repeat
E22 Action:T25 Acts-on:T43
T26 Action 1086 1092 Repeat
E23 Action:T26 Acts-on:T44
T2 Modifier 83 92 amplified
T28 Modifier 101 109 filtered
T29 Reagent 110 121 virus stock
T30 Device 433 454 microcentrifuge tubes
T31 Reagent 467 478 supernatant
T32 Reagent 497 503 pellet
T27 Location 134 149 screw cap tubes
T33 Device 161 182 microcentrifuge tubes
T34 Device 344 365 ultracentrifuge tubes
T35 Device 375 385 SW40 rotor
T36 Device 191 212 ultracentrifuge tubes
T37 Modifier 186 190 long
T38 Reagent 232 237 water
T39 Speed 330 333 1 g
R6 Setting Arg1:E4 Arg2:T39
T40 Speed 403 411 133,000g
T41 Time 416 419 1 h
T42 Location 540 553 subdued light
T43 Method 1075 1084 10 and 11
T44 Method 1099 1108 10 and 11
T45 Reagent 990 1001 supernatant
T47 Amount 624 628 1 µL
T48 Reagent 629 634 stain
T49 Modifier 643 655 concentrated
T50 Location 656 666 virus tube
T46 Reagent 601 613 SYBR Green I
R7 Measure Arg1:T48 Arg2:T47
R8 Mod-Link Arg1:T50 Arg2:T49
R9 Mod-Link Arg1:T36 Arg2:T37
T52 Modifier 715 723 staining
T53 Reagent 751 758 viruses
T54 Reagent 784 794 suspension
T55 Location 802 818 microscope slide
R10 Mod-Link Arg1:T53 Arg2:T52
T56 Amount 772 776 1 µL
R11 Measure Arg1:T54 Arg2:T56
R12 Measure Arg1:T29 Arg2:T22
R13 Mod-Link Arg1:T29 Arg2:T28
R1 Measure Arg1:T29 Arg2:T23
R2 Mod-Link Arg1:T29 Arg2:T2
T57 Amount 912 919 1.65 mL
T58 Size 827 837 18 × 18 mm
T59 Device 838 847 coverslip
R3 Measure Arg1:T59 Arg2:T58
T60 Device 864 869 slide
T61 Device 880 906 epifluorescence microscope
T62 Reagent 920 925 water
T63 Reagent 929 937 seawater
T64 Location 953 957 tube
R4 Measure Arg1:T62 Arg2:T57
R14 Measure Arg1:T63 Arg2:T57
R15 Or Arg1:T62 Arg2:T63
T65 Method 517 533 subsequent steps
R16 Setting Arg1:E6 Arg2:T40
R17 Setting Arg1:E6 Arg2:T41
T51 Time 238 305 until they are just flush with the top of the ultracentrifuge tubes
R18 Setting Arg1:E3 Arg2:T51
T24 Amount 127 133 1.7-mL
T66 Time 684 690 15 min
T67 Location 698 702 dark
R5 Setting Arg1:E13 Arg2:T66