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protocol_71.ann
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T1 Action 47 54 Prepare
E1 Action:T1 Acts-on:T36
T2 Action 165 174 resuspend
E2 Action:T2 Acts-on:T60 Using:E3 Site:T56
T3 Action 208 214 adding
E3 Action:T3 Site:T59
T4 Action 327 333 Filter
E4 Action:T4 Acts-on:T61 Using:T63
T5 Action 372 382 centrifuge
E5 Action:T5 Acts-on:T61
T6 Action 413 422 resuspend
E6 Action:T6 Acts-on:T61 Site:T67
T7 Action 468 473 Count
E7 Action:T7 Acts-on:T68
T8 Action 478 484 adjust
E8 Action:T8 Acts-on:T68
T9 Action 529 536 Aliquot
E9 Action:T9 Acts-on:T32 Site:T33
T10 Action 592 595 Add
E10 Action:T10 Acts-on:T72
T11 Action 635 638 mix
E11 Action:T11 Acts-on:T72
T12 Action 648 656 incubate
E12 Action:T12 Acts-on:T72 Site:T74
T13 Action 680 685 Scale
E13 Action:T13 Acts-on:T76
T15 Action 751 754 add
E15 Action:T15 Acts-on:T55
T16 Action 961 970 Resuspend
E16 Action:T16 Using:E17 Acts-on:T14
T17 Action 984 993 vortexing
E17 Action:T17
T18 Action 1021 1024 Add
E18 Action:T18 Acts-on:T80
T19 Action 1058 1061 Mix
E19 Action:T19
T20 Action 1071 1079 incubate
E20 Action:T20 Site:T83
T21 Action 1103 1108 Scale
E21 Action:T21 Acts-on:T84
T23 Action 1279 1283 Wash
E23 Action:T23 Acts-on:T52 Using:E24
T24 Action 1297 1303 adding
E24 Action:T24 Acts-on:T53
T25 Action 1330 1340 centrifuge
E25 Action:T25 Acts-on:T53
T26 Action 1367 1374 discard
E26 Action:T26 Acts-on:T51
T27 Action 1481 1490 Resuspend
E27 Action:T27 Acts-on:T46 Site:T22
T28 Action 1639 1648 pipetting
E28 Action:T28 Acts-on:T44 Using:T45
T29 Action 1613 1620 disrupt
E29 Action:T29 Acts-on:T43 Using:E28
T30 Action 1675 1680 Place
E30 Action:T30 Acts-on:T40 Site:T41
T31 Action 1719 1723 Pour
E31 Action:T31 Acts-on:T39
T36 Reagent 55 60 cells
T38 Action 1732 1739 collect
E36 Action:T38 Acts-on:T39
T39 Reagent 1744 1750 liquid
T40 Device 1685 1689 tube
T41 Location 1697 1703 magnet
T42 Time 1708 1717 5 minutes
R1 Setting Arg1:E30 Arg2:T42
T43 Reagent 1625 1635 aggregates
T44 Reagent 1653 1661 solution
T45 Method 1662 1673 up and down
T46 Reagent 1495 1500 cells
T47 Amount 1504 1508 3 mL
T48 Amount 1304 1308 3 mL
T49 Speed 1344 1351 300 x g
T50 Time 1356 1365 5 minutes
T51 Reagent 1375 1386 supernatant
T52 Reagent 1288 1293 cells
T53 Reagent 1312 1328 MojoSort™ Buffer
R3 Measure Arg1:T53 Arg2:T48
R2 Setting Arg1:E25 Arg2:T49
R4 Setting Arg1:E25 Arg2:T50
T54 Amount 755 761 100 μL
T55 Reagent 766 779 1 x 108 cells
R5 Measure Arg1:T55 Arg2:T54
T56 Location 188 204 MojoSort™ Buffer
T57 Amount 221 224 4mL
T58 Amount 230 234 5 mL
T59 Location 248 264 polystyrene tube
T60 Reagent 179 184 cells
R7 Measure Arg1:T60 Arg2:T57
T37 Reagent 71 89 tissue of interest
R8 Meronym Arg1:T36 Arg2:T37
T61 Reagent 338 343 cells
T62 Size 351 356 70 μm
T63 Device 357 370 cell strainer
R9 Setting Arg1:T63 Arg2:T62
T64 Speed 386 393 300 x g
T65 Time 398 407 5 minutes
R10 Setting Arg1:E5 Arg2:T64
R11 Setting Arg1:E5 Arg2:T65
T66 Modifier 426 446 anappropriate volume
T67 Reagent 450 466 MojoSort™ Buffer
R12 Mod-Link Arg1:T67 Arg2:T66
T68 Reagent 489 507 cell concentration
T69 Concentration 511 527 1 x 108 cells/mL
R13 Setting Arg1:E8 Arg2:T69
R14 Setting Arg1:E7 Arg2:T69
T70 Amount 537 543 100 μL
T71 Amount 596 601 10 μL
T72 Reagent 609 633 Biotin-Antibody Cocktail
R15 Measure Arg1:T72 Arg2:T71
T73 Modifier 639 643 well
R16 Mod-Link Arg1:E11 Arg2:T73
T74 Location 660 663 ice
T75 Time 668 678 15 minutes
R17 Setting Arg1:E12 Arg2:T75
T76 Reagent 693 699 volume
T77 Modifier 700 711 accordingly
R18 Mod-Link Arg1:E13 Arg2:T77
T14 Reagent 975 980 beads
T78 Speed 995 1019 maximum speed, 5 touches
T79 Amount 1025 1030 10 μL
T80 Reagent 1034 1056 Streptavidin Nanobeads
R20 Measure Arg1:T80 Arg2:T79
T81 Modifier 1062 1066 well
R21 Mod-Link Arg1:E19 Arg2:T81
T82 Time 1091 1101 15 minutes
T83 Location 1083 1086 ice
R22 Setting Arg1:E20 Arg2:T82
T84 Reagent 1116 1122 volume
T85 Modifier 1123 1134 accordingly
R23 Mod-Link Arg1:E21 Arg2:T85
T22 Location 1512 1528 MojoSort™ Buffer
R24 Measure Arg1:T22 Arg2:T47
R6 Measure Arg1:T59 Arg2:T58
T32 Reagent 547 562 cell suspension
T33 Location 586 590 tube
T34 Modifier 582 585 new
R25 Measure Arg1:T32 Arg2:T70
R26 Mod-Link Arg1:T33 Arg2:T34
R19 Setting Arg1:E17 Arg2:T78