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<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="UTF-8">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<title>David Garcia - Senior Research Scientist</title>
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</head>
<body>
<header>
<h1>David Garcia</h1>
<img src="portrait_DG.png" alt="Profile Picture">
<h3 style="text-align: center; margin-top: 1rem; font-weight: normal;">Senior Research Scientist<br>DEVCOM CBC & Caltech</h3>
<div class="social-links">
<a href="https://www.linkedin.com/in/david-garcia-172602ba/" target="_blank">
<img src="https://upload.wikimedia.org/wikipedia/commons/c/ca/LinkedIn_logo_initials.png" alt="LinkedIn">
LinkedIn
</a>
<a href="https://scholar.google.com/citations?user=HTLatcMAAAAJ&hl=en" target="_blank">
<img src="https://upload.wikimedia.org/wikipedia/commons/c/c7/Google_Scholar_logo.svg" alt="Google Scholar">
Google Scholar
</a>
</div>
<nav>
<ul>
<li><a href="#biography">Biography</a></li>
<li><a href="#research">Research</a></li>
<li><a href="#publications">Publications</a></li>
</ul>
</nav>
</header>
<main>
<section id="biography">
<h2>Biography</h2>
<p>Dr. David Garcia is a synthetic biologist and metabolic engineer working as Senior Research Scientist in the applied synthetic biology branch at
<a href="https://www.cbc.devcom.army.mil/research/biosciences/"
target="_blank" style="color: rgb(53, 53, 87); text-decoration: underline;">DEVCOM CBC</a> and is currently
at <a href="https://murray.cds.caltech.edu/Main_Page" target="_blank" style="color: rgb(53, 53, 87); text-decoration: underline;">Caltech</a>
under the guidance of Dr. Richard Murray. Prior to his current position he was a National Research Council Fellow at
Caltech in the Murray lab developing cell-free biological circuitry and simplified organisms. He earned his doctorate in
Mitchel J Doktycz’s laboratory at The University of Tennessee and Oak Ridge National Laboratory. During this time, he was awarded an NSF Graduate Research Fellowship to
develop his thesis work focused on cell-free systems optimized for metabolic engineering and protein production. His current work is centered around the use of
high-throughput methodologies to scale the production of cell-free biological sensors and cell-free bioproduction.</p>
<div style="display: flex; justify-content: flex-end; gap: 1rem; padding-right: rem; margin-top: 50px;">
<a href="David_Garcia_CV.pdf" class="download-link" download="David_Garcia_CV.pdf">Download CV</a>
<!-- Outreach Button -->
<a href="#" class="download-link" onclick="showMentoringModal(); return false;">Mentoring</a>
<a href="#" class="download-link" onclick="showOutreachModal(); return false;">Outreach</a>
<a href="#" class="download-link" onclick="showAwardsModal(); return false;">Awards</a>
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<div class="modal-content" style="width: 100%; max-width: 1000px; padding: 50px; background-color: white; border-radius: 8px; margin: 40px auto;">
<span class="close" onclick="closeMentoringModal();" style="cursor: pointer; font-size: 18px; float: right;">×</span>
<h3 style="font-size: 1.2rem; margin-top: 0;">Mentoring Activities</h3>
<p>As an academic, I believe mentoring is the foundational cornerstone of our profession. Becoming a professor not only offers the opportunity create new knowledge, but the ability to
share it with my students. My interest in academia is rooted in the strong mentorship I received both as a student and as a young researcher. The work of my mentors inspired me to develop
a robust and hands-on approach rooted in developing a strong fundemental understanding of the basics of a project while encouraging students to take ownership of their work explore their own insights.
During my career I’ve mentored four junior graduate students, 10 undergraduates, and many more on my iGEM team. My focus has always been on understanding each student's needs, providing hands-on
guidance, and creating an eviroment where each student can thrive. As someone who loves to discuss science, I nurture my students’ interest and monitor their progress by encouraging frequent casual
meetings in between more formalized presentations and reports. I have found that providing feedback on a student’s ability to describe their work either through talks or written reports allows me to
effectively steer a project and provides them with a more holistic view of the mechanics and purpose of their work.</p>
<ul style="text-align: left; font-size: 1rem; line-height: 1.5rem; padding: 0; list-style: none;">
<li><strong>2024:</strong> Caltech Summer Undergraduate Fellow Mentor (SURF) (Max Janssen)</li>
<li><strong>2024:</strong> Caltech Diversification Initiative through Veteran Education(DIVE) (Hanna Kashchei) (Lisardo Duran Jr.)</li>
<li><strong>2023:</strong> DEVCOM CBC Army Educational Outreach Mentor (AEOP)(Alexandra Minor) </li>
<li><strong>2021:</strong> Caltech Summer Undergraduate Fellow Mentor (SURF) (Pranay Satya)</li>
<li><strong>2015-2018:</strong> IGEM Graduate Student Advisor and Founder:</strong> Responsible for assisting an undergraduate team of researchers design, perform, and present a novel work at the International Genetically Engineered Machine competition.</li>
<li><strong>2017:</strong>IGEM Giant Jamboree Judge:</strong> Judged student presentations and posters at IGEM conference.</li>
</ul>
</div>
</div>
<!-- Awards Modal Structure -->
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<div class="modal-content" style="width: 100%; max-width: 1000px; padding: 50px; background-color: white; border-radius: 8px; margin: 40px auto;">
<span class="close" onclick="closePopup();" style="cursor: pointer; font-size: 18px; float: right;">×</span>
<h3 style="font-size: 1.2rem; margin-top: 0;">Fellowships, Awards, and Grants</h3>
<ul style="text-align: left; font-size: 1rem; line-height: 1.5rem; padding: 0; list-style: none;">
<li><strong>2022-Present:</strong> Grant: Principal Investigator: DEVCOM CBC Laboratory Independent Research Program ($405K)</li>
<li><strong>2022-Present:</strong> Grant: Principal Investigator: DEVCOM CBC Laboratory Independent Research Program ($405K)</li>
<li><strong>2021-Present:</strong> Fellowship: National Research Council Postdoctoral Fellowship</li>
<li><strong>2020:</strong> Award: ORNL (Biosciences Division) Distinguished Achievement Award</li>
<li><strong>2020:</strong> Award: UT Extraordinary Professional Promise Award</li>
<li><strong>2017-2020:</strong> Fellowship: National Science Foundation Graduate Research Fellowship</li>
<li><strong>2017:</strong> Award: Forum on Science Ethics and Policy Writing Competition 1st Place</li>
<li><strong>2016:</strong> Award: GEM Consortium Conference 2016: 1st Place Poster Award</li>
<li><strong>2016:</strong> Award: 2016 National Science Foundation Conference Travel Grant</li>
<li><strong>2015-16:</strong> Fellowship: Energy Science & Engineering, Bredesen Center for Interdisciplinary Research and Graduate Education Fellowship, University of Tennessee</li>
<li><strong>2015-16:</strong> Fellowship: GEM Consortium Graduate Fellowship</li>
</ul>
</div>
</div>
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<span class="close" onclick="closeOutreachModal();" style="cursor: pointer; font-size: 18px; float: right;">×</span>
<h3 style="font-size: 1.2rem; margin-top: 0;">Outreach Activities</h3>
<ul style="text-align: left; font-size: 1rem; line-height: 1.5rem; padding: 0; list-style: none;">
<li><strong>Synthetic Biology Young Speaker Series Conference Organizing Committee:</strong> Assisting with organizing and funding the first iteration of the Synthetic Biology Young Speaker Series conference. (2023-Present)</li>
<li><strong>Synthetic Biology Gordon Research Seminar Elected Chair:</strong> Responsible for funding and organizing Gordon research seminar. (2019-2023)</li>
<li><strong>Daily Beacon Science Columnist:</strong> Produced a science and society column dedicated to helping bridge the divide between science and the public through discussions of scientific events and topics. (2015-2017)</li>
</ul>
</div>
</div>
</section>
<section id="research">
<h2>Research</h2>
<p>Engineerable biological systems hold immense promise in addressing a wide range of challenges, from combating biological and chemical threats to improving human health,
food security, and material production. The complexity of even simple programs within the context of a cell inherently requires minimization into a modular engineerable context.
By understanding the fundamental components of complex biological systems and their interactions, we can create biological tools
that operate with robustness, predictability, and precision. My work focuses on utilizing cell-free synthetic biology to conduct high-throughput characterization of these
systems, aiming to both deepen our understanding of their core functions and enhance their potential for novel biological sensing and production mechanisms.</p>
<div class="icons">
<!-- Modal 1 Icon and Link -->
<div class="icon">
<a href="#" onclick="openModal('modal1'); return false;" style="text-align: center; display: inline-block;">
<img src="research_area_icon_1.png" alt="Icon 1" style="display: block; margin: 0 auto;">
<span style="display: block; white-space: nowrap; margin-top: 5px;">Biological Sensing</span>
</a>
</div>
<!-- Modal 1 Content -->
<div id="modal1" class="modal" style="display: none; position: fixed; z-index: 9999; left: 0; top: 0; width: 100%; height: 100%; background-color: rgba(0, 0, 0, 0.8); justify-content: center; align-items: center;">
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<span class="close-modal" style="position: absolute; top: 1rem; right: 1rem; font-size: 1.5rem; cursor: pointer;">×</span>
<img src="Biosensor Optimization.png" alt="Biological Sensing Image" style="width: 80%; height: auto; margin-bottom: 1rem; border-radius: 8px;">
<p style="font-size: 1.5rem;">A. Graphic illustration of the DBTL cycle used to explore the combinatorial space of cell-free paper-ticket compositions. Initial tests of excipients and reagents defined an initial
DOE matrix that was tested and the resulting data used to define a predictive model for active and optimal reagent concentrations. B. Automated image analysis identifies, crops, detects, and trace hue change per well.
C. Wax printed paper microfluidic device containing cell-free extract optimized using DBTL cycle,. Sample chamber contains nucleic acid cell-free biosensor. Custom software used to acquire traces of hue change </p>
</div>
</div>
<!-- Modal 2 Icon and Link -->
<div class="icon">
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<img src="research_area_icon_2.png" alt="Icon 2" style="display: block; margin: 0 auto;">
<span style="display: block; white-space: nowrap; margin-top: 5px;">Machine Learning Guided<br>Bioprototyping</span>
</a>
</div>
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<img src="ML_Materials_Graphic.png" alt="Machine Learning Guided Bioprototyping Image" style="width: 80%; height: auto; margin-bottom: 1rem; border-radius: 8px;">
<p style="font-size: 1.5rem;">High-throughput computational and cell-free synthetic biology techniques to rapidly test novel substrates as building blocks for new melanin-like materials. Data from protein
screening, molecular simulations, and polymerization screens are aggregated into ML models to make valuable predictions for new substrates and catalysts..</p>
</div>
</div>
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<span style="display: block; white-space: nowrap; margin-top: 5px;">In Vitro Metabolic<br>Engineering</span>
</a>
</div>
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<img src="metabolism_graphic.png" alt="In Vitro Metabolic Engineering Image" style="width: 80%; height: auto; margin-bottom: 1rem; border-radius: 8px;">
<p style="font-size: 1.5rem;">Simplified cell-free model. Products of glycolysis (red) and shikimate pathway(green) are modified,
the effects rapidly measured in real time, and used to inform model parameterization.</p>
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<p> All together my lab is focused on unlocking the power of modular biological systems to create data-driven solutions and insights for biological production and function.
Our goal is to ultimately bring forward solutions for sustainable biomanufacturing, fieldable diagnostics, and personalized medicine.
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<h2>Publications</h2>
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<p>For all full list of publications please visit my <a href="https://scholar.google.com/citations?user=HTLatcMAAAAJ&hl=en" target="_blank">Google Scholar</a>.</p>
<li><a href="https://academic.oup.com/synbio/article/3/1/ysy006/4995850" target="_blank">Garcia, D.C., Mohr, B., Dovgan, J. T., Hurst, G. B., Standaert, R. F., and Doktycz, M. J.
Elucidating the potential of crude cell extracts for producing pyruvate from glucose. Synthetic Biology. (2018) SynBio</em>.</a></li>
<li><a href="https://www.sciencedirect.com/science/article/pii/S221403012100002X" target="_blank">Garcia, D.C.; Dinglasan, Jaime L.N.; Shrestha, H.; Abraham, P.E.; Hettich, R.L.; Doktycz, M.J.;
A lysate proteome engineering strategy for enhancing cell-free metabolite production. (2020). <em>Metabolic engineering communications.</em>.</a></li>
<li><a href="https://journals.asm.org/doi/full/10.1128/aem.01185-18" target="_blank">Cecil, J. H.; Garcia, D. C.; Giannone, R. J.; Michener, J. K.
Rapid, Parallel Identification of Catabolism Pathways of Lignin-Derived Aromatic Compounds in Novosphingobium Aromaticivorans. Appl. Environ. Microbiol. (2018) <em>Appl. Environ. Microbiol</em>.</a></li>
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