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chaining- rerun with new version #2

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jackhump opened this issue Feb 24, 2020 · 1 comment
Open

chaining- rerun with new version #2

jackhump opened this issue Feb 24, 2020 · 1 comment

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@jackhump
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be strict,

chain_samples.py --dun-merge-5-shorter --max_fuzzy_junction=0 --max_3_diff=30
@jackhump
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scratch that.

use dev version of cupcake: https://github.com/Magdoll/cDNA_Cupcake/tree/dev

first cupcake collapse (without dun-merge) to collapse 5' degraded transcripts and count abundances

Then do chaining, again without dun-merge

Then do a final round of collapsing (without dun-merge) to now collapse 5' degraded (but full length in that sample) reads into hopefully the longest read across all transcripts.

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