diff --git a/src/gg.regression.unity.bots/Runtime/Scripts/RGHeadlessSequenceRunner.cs b/src/gg.regression.unity.bots/Runtime/Scripts/RGHeadlessSequenceRunner.cs
index efef3919..1b1e7301 100644
--- a/src/gg.regression.unity.bots/Runtime/Scripts/RGHeadlessSequenceRunner.cs
+++ b/src/gg.regression.unity.bots/Runtime/Scripts/RGHeadlessSequenceRunner.cs
@@ -1,5 +1,6 @@
 using System;
 using System.Collections;
+using System.IO;
 using System.Linq;
 using System.Reflection;
 using RegressionGames.StateRecorder.BotSegments;
@@ -23,6 +24,7 @@ public static class RGHeadlessSequenceRunner
         internal static readonly int Rc_SequenceTimeoutNeedsPath = 5;
         internal static readonly int Rc_SequenceTimeoutMissing = 6;
         internal static readonly int Rc_SequenceTimeoutNotInt = 7;
+        internal static readonly int Rc_SequencePathNotRelative = 8;
 
         internal static readonly string SequencePathArgument = "-rgsequencepath";
         internal static readonly string SequenceTimeoutArgument = "-rgsequencetimeout";
@@ -124,7 +126,19 @@ internal static string ParsePathArgument()
                 {
                     if (i + 1 < argsLength)
                     {
-                        return args[i + 1];
+
+                        var path = args[i + 1];
+                        
+                        // Sequences that are run via -rgsequencepath must be relative, as we only allows sequences
+                        // included in the Resource or persistent path directories.
+                        if (Path.IsPathRooted(path))
+                        {
+                            RGDebug.LogError($"{SequencePathArgument} command line argument requires a relative path");
+                            Application.Quit(Rc_SequencePathNotRelative);
+                            return null;
+                        }
+                        
+                        return path;
                     }
                     // else
                     RGDebug.LogError($"{SequencePathArgument} command line argument requires a path value to be passed after it");
diff --git a/src/gg.regression.unity.bots/Runtime/Scripts/StateRecorder/BotSegments/Models/BotSequence.cs b/src/gg.regression.unity.bots/Runtime/Scripts/StateRecorder/BotSegments/Models/BotSequence.cs
index 1d3db1be..9a78cea1 100644
--- a/src/gg.regression.unity.bots/Runtime/Scripts/StateRecorder/BotSegments/Models/BotSequence.cs
+++ b/src/gg.regression.unity.bots/Runtime/Scripts/StateRecorder/BotSegments/Models/BotSequence.cs
@@ -46,11 +46,6 @@ public static (string, string, BotSequence) LoadSequenceJsonFromPath(string path
                 return (null, null, null);
             }
 
-            if (path.StartsWith('/') || path.StartsWith('\\'))
-            {
-                throw new Exception("Invalid path.  Path must be relative, not absolute in order to support editor vs production runtimes interchangeably.");
-            }
-
             path = path.Replace('\\', '/');
 
             (string, string, string) sequenceJson;
@@ -144,10 +139,6 @@ private static object ParseSegmentOrListJson(string resourcePath, string fileDat
          */
         public static (string, string, object) LoadBotSegmentOrBotSegmentListFromPath(string path)
         {
-            if (path.StartsWith('/') || path.StartsWith('\\'))
-            {
-                throw new Exception("Invalid path.  Path must be relative, not absolute, in order to support editor vs production runtimes interchangeably.");
-            }
 
             path = path.Replace('\\', '/');