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Here you will find information on individual scripts as well as a tutorial for processing your data. Links to akutils wiki pages are found in the sidebar. Other useful links can be found below.
Using akutils:
Executing any akutils command with no arguments will bring up a usage screen. There are also help screens and this wiki. For a basic walk-through, try the tutorial.
Getting akutils:
See the README file which has detailed documentation: akutils-v1.2 README.
Getting akutils (short instructions):
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Install Ubuntu14.04LTS. I prefer 14.04.1, and then upgrade manually (step 3).
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If in a VirtualBox, run this command to allow your screen resolution to expand to your host hardware:
sudo apt-get install virtualbox-guest-x11
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Run all updates. Don't add anything else just yet.
sudo apt-get update
sudo apt-get -yfm upgrade -
Run the akutils_ubuntu_installation script and follow instructions there.
Useful links:
akutils test data
QIIME
Phyloseq
Vsearch
Swarm
Ghost-tree
NCBI Nucleotide BLAST
HTML reverse/complement tool
Fastx toolkit
ea-utils
Veusz open source plotting software
GraphPad QuickCalcs online stats and such
Citing akutils:
Lela V. Andrews. (2018). akutils-v1.2.4: Facilitating analyses of microbial communities through QIIME. Zenodo. 10.5281/zenodo.1205079
Script wiki pages:
akutils
akutils align_and_tree
akutils check
akutils check_result
akutils configure
akutils core diversity
akutils format_database
akutils join_paired_reads
akutils phix_filtering
akutils pick_otus
akutils primer_file
akutils primer_list
akutils print_config
akutils strip_primers
akutils test
akutils test_result
akutils update
ancomR.sh
biom-summarize_folder.sh
biomtotxt.sh
concatenate_fastqs.sh
convert_table_for_ancomR.sh
fastq_data.sh
fasta_length_histogram.sh
fastq_length_histogram.sh
filter_fasta_by_length.sh
filter_fastq_by_length.sh
filter_observations_by_sample.sh
indicator_species.sh
ITSx_parallel.sh
mapcats.sh
preprocess_otus_for_ghost-tree.sh
slurm_builder.sh
synthetic_index.sh
two-way_permanova.sh
txttobiom.sh
unwrap_fasta.sh
Tutorial pages:
akutils tutorial
ITS analysis
slurm usage
Running akutils on monsoon
Example: 2x300, 2 loci
akutils wiki home:
akutils wiki home
akutils home page:
akutils home page
akutils github page:
akutils github page