diff --git a/browser/decentralized_dns/test/BUILD.gn b/browser/decentralized_dns/test/BUILD.gn
index 32f4550ec48a..fe40c71a4fdd 100644
--- a/browser/decentralized_dns/test/BUILD.gn
+++ b/browser/decentralized_dns/test/BUILD.gn
@@ -4,6 +4,7 @@
 # You can obtain one at http://mozilla.org/MPL/2.0/. */
 
 import("//brave/build/config.gni")
+import("//brave/components/tor/buildflags/buildflags.gni")
 import("//testing/test.gni")
 
 source_set("browser_tests") {
@@ -29,6 +30,7 @@ source_set("browser_tests") {
 source_set("unit_tests") {
   testonly = true
   sources = [
+    "//brave/browser/decentralized_dns/test/decentralized_dns_navigation_throttle_unittest.cc",
     "//brave/browser/decentralized_dns/test/utils_unittest.cc",
     "//brave/net/dns/dns_transaction_unittest.cc",
   ]
@@ -37,6 +39,7 @@ source_set("unit_tests") {
     "//base",
     "//base/test:test_support",
     "//brave/components/decentralized_dns",
+    "//brave/components/tor/buildflags",
     "//chrome/test:test_support",
     "//components/prefs",
     "//net",
@@ -44,4 +47,8 @@ source_set("unit_tests") {
     "//testing/gmock",
     "//testing/gtest",
   ]
+
+  if (enable_tor) {
+    deps += [ "//brave/browser/tor" ]
+  }
 }  # source_set("unit_tests")
diff --git a/browser/decentralized_dns/test/decentralized_dns_navigation_throttle_unittest.cc b/browser/decentralized_dns/test/decentralized_dns_navigation_throttle_unittest.cc
new file mode 100644
index 000000000000..f4a04035e9cc
--- /dev/null
+++ b/browser/decentralized_dns/test/decentralized_dns_navigation_throttle_unittest.cc
@@ -0,0 +1,115 @@
+/* Copyright (c) 2021 The Brave Authors. All rights reserved.
+ * This Source Code Form is subject to the terms of the Mozilla Public
+ * License, v. 2.0. If a copy of the MPL was not distributed with this file,
+ * You can obtain one at http://mozilla.org/MPL/2.0/. */
+
+#include "brave/components/decentralized_dns/decentralized_dns_navigation_throttle.h"
+
+#include "base/test/scoped_feature_list.h"
+#include "brave/components/decentralized_dns/features.h"
+#include "brave/components/tor/buildflags/buildflags.h"
+#include "chrome/browser/profiles/profile.h"
+#include "chrome/test/base/testing_browser_process.h"
+#include "chrome/test/base/testing_profile.h"
+#include "chrome/test/base/testing_profile_manager.h"
+#include "components/prefs/testing_pref_service.h"
+#include "content/public/test/browser_task_environment.h"
+#include "content/public/test/mock_navigation_handle.h"
+#include "content/public/test/test_renderer_host.h"
+#include "content/public/test/web_contents_tester.h"
+#include "testing/gtest/include/gtest/gtest.h"
+
+#if BUILDFLAG(ENABLE_TOR)
+#include "brave/browser/tor/tor_profile_manager.h"
+#endif
+
+constexpr char kTestProfileName[] = "TestProfile";
+
+namespace decentralized_dns {
+
+class DecentralizedDnsNavigationThrottleTest : public testing::Test {
+ public:
+  DecentralizedDnsNavigationThrottleTest()
+      : profile_manager_(TestingBrowserProcess::GetGlobal()) {}
+  ~DecentralizedDnsNavigationThrottleTest() override = default;
+
+  void SetUp() override {
+    feature_list_.InitAndEnableFeature(features::kDecentralizedDns);
+
+    ASSERT_TRUE(profile_manager_.SetUp());
+    profile_ = profile_manager_.CreateTestingProfile(kTestProfileName);
+    local_state_ = profile_manager_.local_state();
+    web_contents_ =
+        content::WebContentsTester::CreateTestWebContents(profile_, nullptr);
+    locale_ = "en-US";
+  }
+
+  void TearDown() override { web_contents_.reset(); }
+
+  PrefService* local_state() { return local_state_->Get(); }
+  content::WebContents* web_contents() { return web_contents_.get(); }
+
+  // Helper that creates simple test guest profile.
+  Profile* CreateGuestProfile() {
+    return profile_manager_.CreateGuestProfile()->GetPrimaryOTRProfile();
+  }
+
+  TestingProfile* profile() { return profile_; }
+
+  const std::string& locale() { return locale_; }
+
+ private:
+  content::BrowserTaskEnvironment task_environment_;
+  content::RenderViewHostTestEnabler test_render_host_factories_;
+  ScopedTestingLocalState* local_state_;
+  TestingProfileManager profile_manager_;
+  TestingProfile* profile_;
+  base::test::ScopedFeatureList feature_list_;
+  std::unique_ptr<content::WebContents> web_contents_;
+  std::string locale_;
+};
+
+TEST_F(DecentralizedDnsNavigationThrottleTest, Instantiation) {
+  content::MockNavigationHandle test_handle(web_contents());
+  auto throttle = DecentralizedDnsNavigationThrottle::MaybeCreateThrottleFor(
+      &test_handle, local_state(), locale());
+  EXPECT_TRUE(throttle != nullptr);
+
+  // Disable in OTR profile.
+  auto otr_web_contents = content::WebContentsTester::CreateTestWebContents(
+      profile()->GetPrimaryOTRProfile(), nullptr);
+  content::MockNavigationHandle otr_test_handle(otr_web_contents.get());
+  auto throttle_in_otr =
+      DecentralizedDnsNavigationThrottle::MaybeCreateThrottleFor(
+          &otr_test_handle, local_state(), locale());
+  EXPECT_EQ(throttle_in_otr, nullptr);
+
+  // Disable in guest profiles.
+  auto* guest_profile = CreateGuestProfile();
+  auto guest_web_contents =
+      content::WebContentsTester::CreateTestWebContents(guest_profile, nullptr);
+  content::MockNavigationHandle guest_test_handle(guest_web_contents.get());
+  auto throttle_in_guest =
+      DecentralizedDnsNavigationThrottle::MaybeCreateThrottleFor(
+          &guest_test_handle, local_state(), locale());
+  EXPECT_EQ(throttle_in_guest, nullptr);
+}
+
+#if BUILDFLAG(ENABLE_TOR)
+TEST_F(DecentralizedDnsNavigationThrottleTest, NotInstantiatedInTor) {
+  Profile* tor_profile =
+      TorProfileManager::GetInstance().GetTorProfile(profile());
+  ASSERT_TRUE(tor_profile->IsTor());
+  ASSERT_TRUE(tor_profile->IsOffTheRecord());
+
+  auto tor_web_contents =
+      content::WebContentsTester::CreateTestWebContents(tor_profile, nullptr);
+  content::MockNavigationHandle tor_test_handle(tor_web_contents.get());
+  auto throttle_in_tor =
+      DecentralizedDnsNavigationThrottle::MaybeCreateThrottleFor(
+          &tor_test_handle, local_state(), locale());
+  EXPECT_EQ(throttle_in_tor, nullptr);
+}
+#endif
+
+}  // namespace decentralized_dns
diff --git a/browser/profiles/BUILD.gn b/browser/profiles/BUILD.gn
index ed5b9dae5f52..39a22a1ab26c 100644
--- a/browser/profiles/BUILD.gn
+++ b/browser/profiles/BUILD.gn
@@ -53,4 +53,8 @@ source_set("profiles") {
   if (enable_tor) {
     deps += [ "//brave/browser/tor" ]
   }
+
+  if (decentralized_dns_enabled) {
+    deps += [ "//brave/browser/decentralized_dns" ]
+  }
 }
diff --git a/browser/tor/BUILD.gn b/browser/tor/BUILD.gn
index 4cd2a4f5f45d..dc0e793c639d 100644
--- a/browser/tor/BUILD.gn
+++ b/browser/tor/BUILD.gn
@@ -8,6 +8,7 @@ source_set("tor") {
   visibility = [
     ":*",
     "//brave/browser:browser_process",
+    "//brave/browser/decentralized_dns/test:unit_tests",
     "//brave/browser/profiles",
     "//brave/browser/ui",
     "//brave/components/ipfs/test:*",
diff --git a/components/decentralized_dns/decentralized_dns_navigation_throttle.cc b/components/decentralized_dns/decentralized_dns_navigation_throttle.cc
index 10b0fec6127a..6a76a7529968 100644
--- a/components/decentralized_dns/decentralized_dns_navigation_throttle.cc
+++ b/components/decentralized_dns/decentralized_dns_navigation_throttle.cc
@@ -28,7 +28,9 @@ DecentralizedDnsNavigationThrottle::MaybeCreateThrottleFor(
     content::NavigationHandle* navigation_handle,
     PrefService* local_state,
     const std::string& locale) {
-  if (!IsDecentralizedDnsEnabled())
+  content::BrowserContext* context =
+      navigation_handle->GetWebContents()->GetBrowserContext();
+  if (!IsDecentralizedDnsEnabled() || context->IsOffTheRecord())
     return nullptr;
 
   return std::make_unique<DecentralizedDnsNavigationThrottle>(