# ------------------------------------------------------------------ # SearchGUI 4.1.8 Report File # # Originally saved by: 18811 @ LAPTOP-H8OT8EHN # on: 01 4ÔÂ 2022, 20:15 # as: report # ------------------------------------------------------------------ Thu Mar 31 17:17:46 CST 2022 Formatting human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.fasta for OMSSA. Building a new DB, current time: 03/31/2022 17:17:46 New DB name: human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.fasta New DB title: human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.fasta Sequence type: Protein Keep Linkouts: T Keep MBits: T Maximum file size: 1073741824B Adding sequences from FASTA; added 369412 sequences in 25.4594 seconds. Thu Mar 31 17:18:12 CST 2022 Database Formating finished for human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.fasta (25.8 seconds). Thu Mar 31 17:18:12 CST 2022 human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.fasta formatted for OMSSA. Thu Mar 31 17:18:12 CST 2022 Converting raw files. Thu Mar 31 17:18:12 CST 2022 Processing 293t-CELL-DMSO-1.raw with ThermoRawFileParser. Thu Mar 31 17:18:12 CST 2022 Processing 293t-CELL-DMSO-2.raw with ThermoRawFileParser. 2022-03-31 17:18:12 INFO Started parsing D:\All for paper\Microprotein\Search_engine\SearchGUI\293t-CELL-DMSO-1.raw 2022-03-31 17:18:12 INFO Started parsing D:\All for paper\Microprotein\Search_engine\SearchGUI\293t-CELL-DMSO-2.raw 2022-03-31 17:18:27 INFO Processing 39515 MS scans 2022-03-31 17:18:28 INFO Processing 38442 MS scans 2022-03-31 17:20:20 INFO Finished parsing D:\All for paper\Microprotein\Search_engine\SearchGUI\293t-CELL-DMSO-2.raw Thu Mar 31 17:20:20 CST 2022 ThermoRawFileParser finished for 293t-CELL-DMSO-2.raw (2 minutes 8.0 seconds). 2022-03-31 17:20:24 INFO Finished parsing D:\All for paper\Microprotein\Search_engine\SearchGUI\293t-CELL-DMSO-1.raw Thu Mar 31 17:20:24 CST 2022 ThermoRawFileParser finished for 293t-CELL-DMSO-1.raw (2 minutes 13.0 seconds). Thu Mar 31 17:20:25 CST 2022 Raw files conversion completed (2 minutes 13.0 seconds). Thu Mar 31 17:20:25 CST 2022 Importing spectrum files. Thu Mar 31 17:20:25 CST 2022 Importing spectrum file 293t-CELL-DMSO-1.mzml Thu Mar 31 17:20:38 CST 2022 Importing spectrum file 293t-CELL-DMSO-2.mzml Thu Mar 31 17:20:52 CST 2022 Importing spectrum files completed (27.8 seconds). Thu Mar 31 17:20:52 CST 2022 Converting spectrum file 293t-CELL-DMSO-1.mzml to peak list. Thu Mar 31 17:21:04 CST 2022 Processing 293t-CELL-DMSO-1.mgf with X!Tandem. X! TANDEM Vengeance (2015.12.15.2) Loading spectra| (mgf).................. loaded. Spectra matching criteria = 30487 Starting threads .|.|.|.|.|.|.|. started. Computing models: Spectrum-to-sequence matching proce:ss in progress | 50 ks Spectrum-to-sequence matching proces:s in progress | 100 ks Spectrum-to-sequence matching process in progress | 150 ks : Spectrum-to-sequence matching process in progress | 200 ks Spectrum-to-sequen:ce matching process in progress | 250 ks Spectrum-to-sequence matching proc:ess in progress | 300 ks Spectrum-to-sequence matching process in progress | 350 ks Spectrum:-to-sequence waiting for 2|3|4|5|6|7|8| done. sequences modelled = 369 ks Model refinement: partial cleavage ..... done. unanticipated cleavage .:.:.:.:. done. finishing refinement ... done. waiting for 2|3|4|5|6|7|8| done. Merging results: from 2...3...4...5...6...7...8... Creating report: initial calculations ..... done. sorting ..... done. finding repeats ..... done. evaluating results ..... done. calculating expectations ..... done. writing results ..... done. Valid models = 13609 Thu Mar 31 17:40:29 CST 2022 X!Tandem finished for 293t-CELL-DMSO-1.mgf (19 minutes 26.0 seconds). Thu Mar 31 17:40:29 CST 2022 Processing 293t-CELL-DMSO-1.mzml with MS-GF+. MS-GF+ Release (v2022.01.07) (07 January 2022) Java 16.0.1 (Oracle Corporation) Windows 10 (amd64, version 10.0) Loading database files... Warning: Sequence database contains 242 counts of letter 'U', which does not correspond to an amino acid. Warning: Sequence database contains 16508 counts of letter 'X', which does not correspond to an amino acid. Creating the suffix array indexed file... Size: 155942079 AlphabetSize: 28 Suffix creation: 6.22% complete. Suffix creation: 11.93% complete. Suffix creation: 16.93% complete. Suffix creation: 21.29% complete. Suffix creation: 25.78% complete. Suffix creation: 30.20% complete. Suffix creation: 34.31% complete. Suffix creation: 38.09% complete. Suffix creation: 41.55% complete. Suffix creation: 45.85% complete. Suffix creation: 50.21% complete. Suffix creation: 54.44% complete. Suffix creation: 58.48% complete. Suffix creation: 62.84% complete. Suffix creation: 67.01% complete. Suffix creation: 70.92% complete. Suffix creation: 74.58% complete. Suffix creation: 78.55% complete. Suffix creation: 81.83% complete. Suffix creation: 85.67% complete. Suffix creation: 89.52% complete. Suffix creation: 93.50% complete. Suffix creation: 97.02% complete. Sorting suffixes... Size: 17210368 Sorting: 8.13% complete. Sorting: 9.88% complete. Sorting: 17.43% complete. Sorting: 19.76% complete. Sorting: 22.08% complete. Sorting: 23.24% complete. Sorting: 24.40% complete. Sorting: 27.31% complete. Sorting: 29.63% complete. Sorting: 30.80% complete. Sorting: 31.38% complete. Sorting: 34.86% complete. Sorting: 37.77% complete. Sorting: 43.58% complete. Sorting: 44.74% complete. Sorting: 47.06% complete. Sorting: 48.23% complete. Sorting: 49.39% complete. Sorting: 52.88% complete. Sorting: 56.36% complete. Sorting: 62.17% complete. Sorting: 63.33% complete. Sorting: 65.66% complete. Sorting: 67.40% complete. Sorting: 69.73% complete. Sorting: 72.05% complete. Sorting: 73.21% complete. Sorting: 74.37% complete. Sorting: 76.70% complete. Sorting: 77.86% complete. Sorting: 83.67% complete. Sorting: 84.83% complete. Sorting: 94.13% complete. Counting number of distinct peptides in human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.csarr using human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.cnlcp Counting distinct peptides: 8.53% complete. Counting distinct peptides: 16.42% complete. Counting distinct peptides: 24.05% complete. Counting distinct peptides: 31.29% complete. Counting distinct peptides: 38.03% complete. Counting distinct peptides: 44.76% complete. Counting distinct peptides: 50.40% complete. Counting distinct peptides: 56.62% complete. Counting distinct peptides: 63.29% complete. Counting distinct peptides: 68.68% complete. Counting distinct peptides: 74.51% complete. Counting distinct peptides: 81.50% complete. Counting distinct peptides: 88.62% complete. Counting distinct peptides: 96.77% complete. Loading database finished (elapsed time: 168.12 sec) Reading spectra... Opening mzML file 293t-CELL-DMSO-1.mzml Ignoring 0 profile spectra. Ignoring 0 spectra having less than 10 peaks. Reading spectra finished (elapsed time: 216.50 sec) Using 8 threads. Search Parameters: PrecursorMassTolerance: 4.5 ppm IsotopeError: 0,1 TargetDecoyAnalysis: false FragmentationMethod: HCD Instrument: QExactive (Q-Exactive) Enzyme: Tryp Enzyme file: D:\SearchGUI\SearchGUI-4.1.8\resources\temp\search_engines\msgf\params\enzymes.txt Enzyme info: Added new enzyme Trypsin_(no_P_rule) with target residues RK Enzyme info: Added new enzyme CNBr with target residues M Enzyme info: Added new enzyme Pepsin_A with target residues LF Enzyme info: Added new enzyme Thermolysin with target residues AILMFV Enzyme info: Added new enzyme Lys-C_(no_P_rule) with target residues K Enzyme info: Added new enzyme Arg-C_(no_P_rule) with target residues R Enzyme info: Added new enzyme Chymotrypsin_(no_P_rule) with target residues YLFW Enzyme info: Added new enzyme Asp-N_(ambic) with target residues DE Enzyme info: Added new enzyme Arg-N with target residues R Enzyme info: Added new enzyme LysargiNase with target residues RK Protocol: Standard NumTolerableTermini: 2 IgnoreMetCleavage: false MinPepLength: 8 MaxPepLength: 30 MinCharge: 2 MaxCharge: 4 NumMatchesPerSpec: 10 MaxMissedCleavages: 2 MaxNumModsPerPeptide: 2 ChargeCarrierMass: 1.00727649 (proton) MinNumPeaksPerSpectrum: 10 NumIsoforms: 128 Post translational modifications in use: Variable (dynamic): Acetyl on * at the peptide N-terminus (+42.0106) Variable (dynamic): Oxidation on M (+15.9949) Spectrum 0-36241 (total: 36242) Splitting work into 24 tasks. Search progress: 0 / 24 tasks, 0.00% 0.00 seconds elapsed Loading built-in param file: HCD_QExactive_Tryp.param Loading built-in param file: HCD_QExactive_Tryp.param Loading built-in param file: HCD_QExactive_Tryp.param Search progress: 0 / 24 tasks, 11.74% 1.00 minutes elapsed Search progress: 0 / 24 tasks, 20.46% 2.00 minutes elapsed Search progress: 1 / 24 tasks, 27.93% 2.42 minutes elapsed Search progress: 2 / 23 tasks, 30.85% 2.59 minutes elapsed Search progress: 3 / 24 tasks, 30.95% 2.62 minutes elapsed Search progress: 4 / 24 tasks, 31.47% 2.67 minutes elapsed Search progress: 5 / 24 tasks, 33.40% 2.81 minutes elapsed Search progress: 6 / 24 tasks, 34.46% 2.91 minutes elapsed Search progress: 7 / 24 tasks, 35.26% 2.95 minutes elapsed Search progress: 7 / 24 tasks, 31.35% 2.95 minutes elapsed Search progress: 8 / 24 tasks, 36.18% 3.00 minutes elapsed Search progress: 8 / 24 tasks, 50.40% 4.00 minutes elapsed Search progress: 8 / 24 tasks, 61.80% 5.00 minutes elapsed Search progress: 9 / 24 tasks, 62.43% 5.06 minutes elapsed Search progress: 10 / 24 tasks, 64.08% 5.35 minutes elapsed Search progress: 11 / 24 tasks, 64.82% 5.41 minutes elapsed Search progress: 12 / 24 tasks, 65.94% 5.52 minutes elapsed Search progress: 13 / 24 tasks, 67.97% 5.72 minutes elapsed Search progress: 14 / 24 tasks, 69.71% 5.85 minutes elapsed Search progress: 14 / 24 tasks, 71.64% 6.00 minutes elapsed Search progress: 15 / 24 tasks, 72.14% 6.02 minutes elapsed Search progress: 16 / 24 tasks, 72.77% 6.06 minutes elapsed Search progress: 16 / 24 tasks, 86.03% 7.00 minutes elapsed Search progress: 17 / 24 tasks, 95.73% 7.81 minutes elapsed Search progress: 18 / 24 tasks, 96.50% 7.93 minutes elapsed Search progress: 19 / 24 tasks, 96.72% 7.93 minutes elapsed Search progress: 19 / 24 tasks, 96.89% 8.00 minutes elapsed Search progress: 20 / 24 tasks, 98.03% 8.38 minutes elapsed Search progress: 21 / 24 tasks, 98.56% 8.51 minutes elapsed Search progress: 22 / 24 tasks, 99.31% 8.74 minutes elapsed Search progress: 23 / 24 tasks, 99.72% 8.79 minutes elapsed Search progress: 24 / 24 tasks, 100.00% 8.83 minutes elapsed Search progress: 24 / 24 tasks, 100.00% 8.83 minutes elapsed Writing results... Writing results finished (elapsed time: 58.55 sec) File: D:\All for paper\Microprotein\Search_engine\SearchGUI\.SearchGUI_temp\293t-CELL-DMSO-1.msgf.mzid MS-GF+ complete (total elapsed time: 805.29 sec) Thu Mar 31 17:53:55 CST 2022 MS-GF+ finished for 293t-CELL-DMSO-1.mzml (13 minutes 26.0 seconds). Thu Mar 31 17:53:56 CST 2022 Processing 293t-CELL-DMSO-1.mgf with OMSSA. Info: Using 8 search threads Info: Sequence=0 Percent=0% Info: Sequence=10000 Percent=2.707% Info: Sequence=20000 Percent=5.41401% Info: Sequence=30000 Percent=8.12101% Info: Sequence=40000 Percent=10.828% Info: Sequence=50000 Percent=13.535% Info: Sequence=60000 Percent=16.242% Info: Sequence=70000 Percent=18.949% Info: Sequence=80000 Percent=21.656% Info: Sequence=90000 Percent=24.363% Info: Sequence=100000 Percent=27.07% Info: Sequence=110000 Percent=29.7771% Info: Sequence=120000 Percent=32.4841% Info: Sequence=130000 Percent=35.1911% Info: Sequence=140000 Percent=37.8981% Info: Sequence=150000 Percent=40.6051% Info: Sequence=160000 Percent=43.3121% Info: Sequence=170000 Percent=46.0191% Info: Sequence=180000 Percent=48.7261% Info: Sequence=190000 Percent=51.4331% Info: Sequence=200000 Percent=54.1401% Info: Sequence=210000 Percent=56.8471% Info: Sequence=220000 Percent=59.5541% Info: Sequence=230000 Percent=62.2611% Info: Sequence=240000 Percent=64.9681% Info: Sequence=250000 Percent=67.6751% Info: Sequence=260000 Percent=70.3821% Info: Sequence=270000 Percent=73.0891% Info: Sequence=280000 Percent=75.7961% Info: Sequence=290000 Percent=78.5031% Info: Sequence=300000 Percent=81.2101% Info: Sequence=310000 Percent=83.9171% Info: Sequence=320000 Percent=86.6241% Info: Sequence=330000 Percent=89.3312% Info: Sequence=340000 Percent=92.0382% Info: Sequence=350000 Percent=94.7452% Info: Sequence=360000 Percent=97.4522% Thu Mar 31 18:33:46 CST 2022 OMSSA finished for 293t-CELL-DMSO-1.mgf (39 minutes 50.0 seconds). Thu Mar 31 18:33:46 CST 2022 Processing 293t-CELL-DMSO-1.mzml with Comet. Comet version "2021.02 rev. 0" Search start: 03/31/2022, 06:33:46 PM - Input file: D:\All for paper\Microprotein\Search_engine\SearchGUI\293t-CELL-DMSO-1.mzml - Load spectra: 29907 - Search progress: 0% 1% 2% 3% 4% 5% 6% 7% 8% 9% 10% 11% 12% 13% 14% 15% 16% 17% 18% 19% 20% 21% 22% 23% 24% 25% 26% 27% 28% 29% 30% 31% 32% 33% 34% 35% 36% 37% 38% 39% 40% 41% 42% 43% 44% 45% 46% 47% 48% 49% 50% 51% 52% 53% 54% 55% 56% 57% 58% 59% 60% 61% 62% 63% 64% 65% 66% 67% 68% 69% 70% 71% 72% 73% 74% 75% 76% 77% 78% 79% 80% 81% 82% 83% 84% 85% 86% 87% 88% 89% 90% 91% 92% 93% 94% 95% 96% 97% 98% 99% - Post analysis: done Search end: 03/31/2022, 06:47:57 PM, 14m:11s Thu Mar 31 18:47:57 CST 2022 Comet finished for 293t-CELL-DMSO-1.mzml (14 minutes 11.0 seconds). Thu Mar 31 18:47:57 CST 2022 Converting spectrum file 293t-CELL-DMSO-2.mzml to peak list. Thu Mar 31 18:48:07 CST 2022 Processing 293t-CELL-DMSO-2.mgf with X!Tandem. X! TANDEM Vengeance (2015.12.15.2) Loading spectra| (mgf).................. loaded. Spectra matching criteria = 31313 Starting threads .|.|.|.|.|.|.|. started. Computing models: Spectrum-to-sequence matching process in progress | 50 ks : Spectrum-to-sequence matching process in progress | 100 ks Spectrum-to-sequence matchi:ng process in progress | 150 ks Spectrum-to-sequence matching process in pr:ogress | 200 ks Spectrum-to-sequence matching process in progress | 250 ks Spectrum-to-sequence :matching process in progress | 300 ks Spectrum-to-sequence matching process :in progress | 350 ks Spectrum-to-sequence waiting for 2|3|4|56|7|8| done. sequences modelled = 369 ks Model refinement: partial cleavage ..... done. unanticipated cleavage .:.:.:.. done. finishing refinement ... done. waiting for 2|3|4|5|6|7|8| done. Merging results: from 2...3...4...5...6...7...8... Creating report: initial calculations ..... done. sorting ..... done. finding repeats ..... done. evaluating results ..... done. calculating expectations ..... done. writing results ..... done. Valid models = 14045 Thu Mar 31 19:04:19 CST 2022 X!Tandem finished for 293t-CELL-DMSO-2.mgf (16 minutes 11.0 seconds). Thu Mar 31 19:04:19 CST 2022 Processing 293t-CELL-DMSO-2.mzml with MS-GF+. MS-GF+ Release (v2022.01.07) (07 January 2022) Java 16.0.1 (Oracle Corporation) Windows 10 (amd64, version 10.0) Loading database files... Warning: Sequence database contains 242 counts of letter 'U', which does not correspond to an amino acid. Warning: Sequence database contains 16508 counts of letter 'X', which does not correspond to an amino acid. Counting number of distinct peptides in human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.csarr using human-openprot-r1_6-refprots+altprots+isoforms_min_1_pep-+uniprot2019_03_01_concatenated_target_decoy.cnlcp Counting distinct peptides: 8.66% complete. Counting distinct peptides: 17.25% complete. Counting distinct peptides: 26.04% complete. Counting distinct peptides: 34.88% complete. Counting distinct peptides: 43.80% complete. Counting distinct peptides: 52.52% complete. Counting distinct peptides: 61.37% complete. Counting distinct peptides: 70.22% complete. Counting distinct peptides: 79.07% complete. Counting distinct peptides: 87.92% complete. Counting distinct peptides: 96.77% complete. Loading database finished (elapsed time: 24.42 sec) Reading spectra... Opening mzML file 293t-CELL-DMSO-2.mzml Ignoring 0 profile spectra. Ignoring 0 spectra having less than 10 peaks. Reading spectra finished (elapsed time: 66.98 sec) Using 8 threads. Search Parameters: PrecursorMassTolerance: 4.5 ppm IsotopeError: 0,1 TargetDecoyAnalysis: false FragmentationMethod: HCD Instrument: QExactive (Q-Exactive) Enzyme: Tryp Enzyme file: D:\SearchGUI\SearchGUI-4.1.8\resources\temp\search_engines\msgf\params\enzymes.txt Enzyme info: Added new enzyme Trypsin_(no_P_rule) with target residues RK Enzyme info: Added new enzyme CNBr with target residues M Enzyme info: Added new enzyme Pepsin_A with target residues LF Enzyme info: Added new enzyme Thermolysin with target residues AILMFV Enzyme info: Added new enzyme Lys-C_(no_P_rule) with target residues K Enzyme info: Added new enzyme Arg-C_(no_P_rule) with target residues R Enzyme info: Added new enzyme Chymotrypsin_(no_P_rule) with target residues YLFW Enzyme info: Added new enzyme Asp-N_(ambic) with target residues DE Enzyme info: Added new enzyme Arg-N with target residues R Enzyme info: Added new enzyme LysargiNase with target residues RK Protocol: Standard NumTolerableTermini: 2 IgnoreMetCleavage: false MinPepLength: 8 MaxPepLength: 30 MinCharge: 2 MaxCharge: 4 NumMatchesPerSpec: 10 MaxMissedCleavages: 2 MaxNumModsPerPeptide: 2 ChargeCarrierMass: 1.00727649 (proton) MinNumPeaksPerSpectrum: 10 NumIsoforms: 128 Post translational modifications in use: Variable (dynamic): Acetyl on * at the peptide N-terminus (+42.0106) Variable (dynamic): Oxidation on M (+15.9949) Spectrum 0-37395 (total: 37396) Splitting work into 24 tasks. Search progress: 0 / 24 tasks, 0.00% 0.00 seconds elapsed Loading built-in param file: HCD_QExactive_Tryp.param Loading built-in param file: HCD_QExactive_Tryp.param Loading built-in param file: HCD_QExactive_Tryp.param Search progress: 0 / 24 tasks, 12.71% 1.00 minutes elapsed Search progress: 1 / 24 tasks, 26.96% 1.81 minutes elapsed Search progress: 2 / 24 tasks, 29.19% 1.88 minutes elapsed Search progress: 2 / 24 tasks, 25.49% 1.89 minutes elapsed Search progress: 3 / 24 tasks, 31.04% 2.00 minutes elapsed Search progress: 4 / 23 tasks, 34.01% 2.14 minutes elapsed Search progress: 5 / 24 tasks, 33.70% 2.15 minutes elapsed Search progress: 6 / 24 tasks, 33.96% 2.15 minutes elapsed Search progress: 7 / 24 tasks, 35.82% 2.24 minutes elapsed Search progress: 8 / 24 tasks, 36.91% 2.29 minutes elapsed Search progress: 8 / 24 tasks, 48.01% 3.00 minutes elapsed Search progress: 8 / 24 tasks, 58.73% 4.00 minutes elapsed Search progress: 9 / 23 tasks, 63.42% 4.09 minutes elapsed Search progress: 9 / 24 tasks, 58.37% 4.21 minutes elapsed Search progress: 11 / 24 tasks, 63.77% 4.39 minutes elapsed Search progress: 12 / 24 tasks, 68.60% 4.85 minutes elapsed Search progress: 12 / 24 tasks, 69.77% 5.00 minutes elapsed Search progress: 13 / 24 tasks, 70.44% 5.09 minutes elapsed Search progress: 13 / 24 tasks, 66.52% 5.10 minutes elapsed Search progress: 15 / 24 tasks, 71.32% 5.17 minutes elapsed Search progress: 16 / 24 tasks, 72.63% 5.27 minutes elapsed Search progress: 16 / 24 tasks, 82.67% 6.00 minutes elapsed Search progress: 17 / 24 tasks, 93.89% 6.88 minutes elapsed Search progress: 17 / 24 tasks, 95.39% 7.00 minutes elapsed Search progress: 18 / 24 tasks, 96.46% 7.09 minutes elapsed Search progress: 19 / 24 tasks, 96.84% 7.13 minutes elapsed Search progress: 20 / 24 tasks, 97.56% 7.34 minutes elapsed Search progress: 21 / 24 tasks, 98.24% 7.52 minutes elapsed Search progress: 22 / 24 tasks, 99.30% 7.87 minutes elapsed Search progress: 23 / 24 tasks, 99.73% 7.94 minutes elapsed Search progress: 24 / 24 tasks, 100.00% 7.98 minutes elapsed Search progress: 24 / 24 tasks, 100.00% 7.98 minutes elapsed Writing results... Writing results finished (elapsed time: 56.30 sec) File: D:\All for paper\Microprotein\Search_engine\SearchGUI\.SearchGUI_temp\293t-CELL-DMSO-2.msgf.mzid MS-GF+ complete (total elapsed time: 602.15 sec) Thu Mar 31 19:14:22 CST 2022 MS-GF+ finished for 293t-CELL-DMSO-2.mzml (10 minutes 3.0 seconds). Thu Mar 31 19:14:22 CST 2022 Processing 293t-CELL-DMSO-2.mgf with OMSSA. Info: Using 8 search threads Info: Sequence=0 Percent=0% Info: Sequence=10000 Percent=2.707% Info: Sequence=20000 Percent=5.41401% Info: Sequence=30000 Percent=8.12101% Info: Sequence=40000 Percent=10.828% Info: Sequence=50000 Percent=13.535% Info: Sequence=60000 Percent=16.242% Info: Sequence=70000 Percent=18.949% Info: Sequence=80000 Percent=21.656% Info: Sequence=90000 Percent=24.363% Info: Sequence=100000 Percent=27.07% Info: Sequence=110000 Percent=29.7771% Info: Sequence=120000 Percent=32.4841% Info: Sequence=130000 Percent=35.1911% Info: Sequence=140000 Percent=37.8981% Info: Sequence=150000 Percent=40.6051% Info: Sequence=160000 Percent=43.3121% Info: Sequence=170000 Percent=46.0191% Info: Sequence=180000 Percent=48.7261% Info: Sequence=190000 Percent=51.4331% Info: Sequence=200000 Percent=54.1401% Info: Sequence=210000 Percent=56.8471% Info: Sequence=220000 Percent=59.5541% Info: Sequence=230000 Percent=62.2611% Info: Sequence=240000 Percent=64.9681% Info: Sequence=250000 Percent=67.6751% Info: Sequence=260000 Percent=70.3821% Info: Sequence=270000 Percent=73.0891% Info: Sequence=280000 Percent=75.7961% Info: Sequence=290000 Percent=78.5031% Info: Sequence=300000 Percent=81.2101% Info: Sequence=310000 Percent=83.9171% Info: Sequence=320000 Percent=86.6241% Info: Sequence=330000 Percent=89.3312% Info: Sequence=340000 Percent=92.0382% Info: Sequence=350000 Percent=94.7452% Info: Sequence=360000 Percent=97.4522% Thu Mar 31 19:54:47 CST 2022 OMSSA finished for 293t-CELL-DMSO-2.mgf (40 minutes 25.0 seconds). Thu Mar 31 19:54:47 CST 2022 Processing 293t-CELL-DMSO-2.mzml with Comet. Comet version "2021.02 rev. 0" Search start: 03/31/2022, 07:54:47 PM - Input file: D:\All for paper\Microprotein\Search_engine\SearchGUI\293t-CELL-DMSO-2.mzml - Load spectra: 30876 - Search progress: 0% 1% 2% 3% 4% 5% 6% 7% 8% 9% 10% 11% 12% 13% 14% 15% 16% 17% 18% 19% 20% 21% 22% 23% 24% 25% 26% 27% 28% 29% 30% 31% 32% 33% 34% 35% 36% 37% 38% 39% 40% 41% 42% 43% 44% 45% 46% 47% 48% 49% 50% 51% 52% 53% 54% 55% 56% 57% 58% 59% 60% 61% 62% 63% 64% 65% 66% 67% 68% 69% 70% 71% 72% 73% 74% 75% 76% 77% 78% 79% 80% 81% 82% 83% 84% 85% 86% 87% 88% 89% 90% 91% 92% 93% 94% 95% 96% 97% 98% 99% - Post analysis: done Search end: 03/31/2022, 08:06:35 PM, 11m:48s Thu Mar 31 20:06:35 CST 2022 Comet finished for 293t-CELL-DMSO-2.mzml (11 minutes 48.0 seconds). Thu Mar 31 20:06:35 CST 2022 Zipping output files. Thu Mar 31 20:09:06 CST 2022 Processing identification files with PeptideShaker. Thu Mar 31 20:13:13 CST 2022 PeptideShaker processing canceled. Please see the PeptideShaker log file: D:\PeptideShaker\PeptideShaker-2.2.6\resources\PeptideShaker.log Thu Mar 31 20:13:13 CST 2022 Searching Canceled! D:\PeptideShaker\PeptideShaker-2.2.6\resources\matches\2022.03.31_DDA20220331-200908.psdb could not be deleted! Thu Mar 31 21:51:47 CST 2022 The search or processing did not finish properly!