diff --git a/README.md b/README.md index f0edde0b..b6b801bd 100755 --- a/README.md +++ b/README.md @@ -11,7 +11,8 @@ Palantir is an algorithm to align cells along differentiation trajectories. Pala $> pip install palantir 2. Palantir depends on a number of `python3` packages available on pypi and these dependencies are listed in `setup.py` -All the dependencies will be automatically installed using the above commands + + All the dependencies will be automatically installed using the above commands 3. To uninstall: @@ -19,7 +20,15 @@ All the dependencies will be automatically installed using the above commands 4. If you would like to determine gene expression trends, please install R programming language and the R package GAM . You will also need to install the rpy2 module using + $> pip install .['PLOT_GENE_TRENDS'] + OR, $> pip install rpy2 + + In case of compiler error during installation of `rpy2`, try to link your compiler in `env`. Example: + + $> env CC=/usr/local/Cellar/gcc/xxx/bin/gcc-x pip install .['PLOT_GENE_TRENDS'] + + where `x` should be replaced with the version numbers 5. Palantir can also be used with [**Scanpy**](https://github.com/theislab/scanpy). It is fully integrated into Scanpy, and can be found under Scanpy's external modules ([link](https://scanpy.readthedocs.io/en/latest/api/scanpy.external.html#external-api)) @@ -70,6 +79,10 @@ ____ Release Notes ------------- +### Version 0.2.6 + + * A fix to [issue#33](https://github.com/dpeerlab/Palantir/issues/33) and [issue#31](https://github.com/dpeerlab/Palantir/issues/31) + ### Version 0.2.5 * A fix related to [issue#28](https://github.com/dpeerlab/Palantir/issues/28). When identifying terminal states, duplicate values were generated instead of unique ones. \ No newline at end of file diff --git a/setup.py b/setup.py index 5c7ef5db..2006bf26 100755 --- a/setup.py +++ b/setup.py @@ -40,9 +40,11 @@ "matplotlib>=2.2.2", "seaborn>=0.8.1", "tzlocal", - "rpy2>=3.0.2", "scanpy", ], + extras_require={ + 'PLOT_GENE_TRENDS': ["rpy2>=3.0.2"] + }, classifiers=[ "Programming Language :: Python :: 3", "License :: OSI Approved :: MIT License", diff --git a/src/palantir/utils.py b/src/palantir/utils.py index 4296900b..d3e533aa 100644 --- a/src/palantir/utils.py +++ b/src/palantir/utils.py @@ -22,11 +22,13 @@ def run_pca(data, n_components=300): return pca_projections, pca.explained_variance_ratio_ -def run_diffusion_maps(data_df, n_components=10, knn=30, n_jobs=-1, alpha=0): +def run_diffusion_maps(data_df, n_components=10, knn=30, alpha=0): """Run Diffusion maps using the adaptive anisotropic kernel - :param data_df: PCA projections of the data or adjancency matrix + :param data_df: PCA projections of the data or adjacency matrix :param n_components: Number of diffusion components + :param knn: Number of nearest neighbors for graph construction + :param alpha: Normalization parameter for the diffusion operator :return: Diffusion components, corresponding eigen values and the diffusion operator """ @@ -36,7 +38,11 @@ def run_diffusion_maps(data_df, n_components=10, knn=30, n_jobs=-1, alpha=0): print("Determing nearest neighbor graph...") temp = sc.AnnData(data_df.values) sc.pp.neighbors(temp, n_pcs=0, n_neighbors=knn) - kNN = temp.uns["neighbors"]["distances"] + # maintaining backwards compatibility to Scanpy `sc.pp.neighbors` + try: + kNN = temp.uns["neighbors"]["distances"] + except KeyError: + kNN = temp.obsp['distances'] # Adaptive k adaptive_k = int(np.floor(knn / 3)) diff --git a/src/palantir/version.py b/src/palantir/version.py index 87fa5448..21a43d46 100644 --- a/src/palantir/version.py +++ b/src/palantir/version.py @@ -1,3 +1,3 @@ -__version__ = "0.2.5" +__version__ = "0.2.6" __author__ = "Manu Setty" __author_email__ = "manu.talanki@gmail.com"