---------------------------------------------------- ,--./,-. ___ __ __ __ ___ /,-._.--~\ |\ | |__ __ / ` / \ |__) |__ } { | \| | \__, \__/ | \ |___ \`-._,-`-, `._,._,' nf-core/chipseq v1.2.2 ---------------------------------------------------- Run Name: disturbed_mahavira #################################################### ## nf-core/chipseq execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: 1. The full error message was: Error executing process > 'PHANTOMPEAKQUALTOOLS (Ss2_Input_R1)' Caused by: Process `PHANTOMPEAKQUALTOOLS (Ss2_Input_R1)` terminated with an error exit status (1) Command executed: RUN_SPP=`which run_spp.R` Rscript -e "library(caTools); source(\"$RUN_SPP\")" -c="Ss2_Input_R1.mLb.clN.sorted.bam" -savp="Ss2_Input_R1.spp.pdf" -savd="Ss2_Input_R1.spp.Rdata" -out="Ss2_Input_R1.spp.out" -p=6 cp spp_correlation_header.txt Ss2_Input_R1_spp_correlation_mqc.tsv Rscript -e "load('Ss2_Input_R1.spp.Rdata'); write.table(crosscorr\$cross.correlation, file=\"Ss2_Input_R1_spp_correlation_mqc.tsv\", sep=",", quote=FALSE, row.names=FALSE, col.names=FALSE,append=TRUE)" awk -v OFS=' ' '{print "Ss2_Input_R1", $9}' Ss2_Input_R1.spp.out | cat spp_nsc_header.txt - > Ss2_Input_R1_spp_nsc_mqc.tsv awk -v OFS=' ' '{print "Ss2_Input_R1", $10}' Ss2_Input_R1.spp.out | cat spp_rsc_header.txt - > Ss2_Input_R1_spp_rsc_mqc.tsv Command exit status: 1 Command output: ################ ChIP data: Ss2_Input_R1.mLb.clN.sorted.bam Control data: NA strandshift(min): -500 strandshift(step): 5 strandshift(max) 1500 user-defined peak shift NA exclusion(min): 10 exclusion(max): NaN num parallel nodes: 6 FDR threshold: 0.01 NumPeaks Threshold: NA Output Directory: . narrowPeak output file name: NA regionPeak output file name: NA Rdata filename: Ss2_Input_R1.spp.Rdata plot pdf filename: Ss2_Input_R1.spp.pdf result filename: Ss2_Input_R1.spp.out Overwrite files?: FALSE Reading ChIP tagAlign/BAM file Ss2_Input_R1.mLb.clN.sorted.bam opened /tmp/RtmpObgHyb/Ss2_Input_R1.mLb.clN.sorted.tagAlign1f5901cb66 done. read 2142350 fragments Command error: Loading required package: Rcpp Error: protect(): protection stack overflow Execution halted Work dir: /external1/PD/nfcore_PD/work/14/16ecaf4e5f071170a3a075c8c46228 Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out` The workflow was completed at 2021-07-15T16:24:16.602042+01:00 (duration: 4h 21m 40s) The command used to launch the workflow was as follows: nextflow run nf-core/chipseq --input design_pe.csv --genome AtlanticSalmon -profile docker -c config_Ss --macs_gsize 2700000000 --narrow_peak True --clip_r1 20 --clip_r2 20 --three_prime_clip_r1 10 --three_prime_clip_r2 10 --max_cpus 16 Pipeline Configuration: ----------------------- - Run Name: disturbed_mahavira - Data Type: Paired-End - Design File: design_pe.csv - Genome: AtlanticSalmon - Fasta File: /external1/PD/genomes/ensembl/Salmo_salar.ICSASG_v2.dna_sm.toplevel.fa - GTF File: /external1/PD/genomes/ensembl/Salmo_salar.ICSASG_v2.104.gtf - BWA Index: /external1/PD/genomes/ensembl/Salmo_salar.ICSASG_v2.dna_sm.toplevel.fa - MACS2 Genome Size: 2700000000 - Min Consensus Reps: 1 - MACS2 Narrow Peaks: Yes - Trim R1: 20 bp - Trim R2: 20 bp - Trim 3' R1: 10 bp - Trim 3' R2: 10 bp - NextSeq Trim: 0 bp - Fingerprint Bins: 500000 - Save Genome Index: No - Max Resources: 128 GB memory, 16 cpus, 10d time per job - Container: docker - nfcore/chipseq:1.2.2 - Output Dir: ./results - Launch Dir: /external1/PD/nfcore_PD - Working Dir: /external1/PD/nfcore_PD/work - Script Dir: /root/.nextflow/assets/nf-core/chipseq - User: root - Config Profile: docker - Date Started: 2021-07-15T12:02:36.359610+01:00 - Date Completed: 2021-07-15T16:24:16.602042+01:00 - Pipeline script file path: /root/.nextflow/assets/nf-core/chipseq/main.nf - Pipeline script hash ID: 39c073c68caf34f6f725e82907fff2bd - Pipeline repository Git URL: https://github.com/nf-core/chipseq - Pipeline repository Git Commit: 6924b669422215f9021144b251e83fc9929be1fe - Pipeline Git branch/tag: master - Nextflow Version: 21.04.2 - Nextflow Build: 5558 - Nextflow Compile Timestamp: 12-07-2021 07:54 UTC -- nf-core/chipseq https://github.com/nf-core/chipseq