Correct format taxanomy header for use with dada2's assignTaxonomy function

[mdehollander]

We managed to create a database with a latest BOLD snapshot and midori+gbol. We are using the dada2 output. When using the merged_dada2_taxonomy.fas file with the assignTaxonomy function in dada2 there is an error: is not a sub-multiple or multiple of the number of rows

The messages in dada2's issue board indicate that the formatting of the file is the issue. The taxonomy string should end with a semicolon. And I think it should have only 6 levels up to genus. So when I removed the species name from each sequence it was accepted and sequences were assigned. Is this something you also experience and can maybe be fixed?

[mdehollander]

Hi @nwnoll, if you have a moment, can you please let me know what you think of the current dada2 output file?

[nwnoll]

Ah sorry... I try to have a look today. Otherwise on the weekend. Anyways.. thanks for reporting!