pairtools 1.0.0 updates #117
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This was referenced Apr 7, 2022
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* in merge, added option to concatenate instead of merge sorted inputs, resolving: #23 * merge checks that columns of inputs are the same
- auto_open defaults to stdin/stdout when path evaluates to False. resolved #48 - auto_open defaults to stdin/stdout when the path is "-" - if the stream is optional, it's controlled by the module itself Warning: this might be unstable because not all the usecases were tested!
Improved version of parse2 with resolved comments from the previous PR: #96 - Separation of parse and parse2 modules. Parse has an option --walks-policy all, which parses long walks, but always reporting pair orientation and outer positions of 5'-ends, as if each pair was read in paired-end mode independently. Parse2 is specifically designed for long walks, and has options --report-position and --report-orientation, which might be used to report junctions, or reads, or walks. - Parse2 has an option to parse single-end reads, --single-end option, tested on minimap2 output for MC-3C. - Parse2 has the max_fragment_size instead instead of parse's max_molecule_size, which help to determine the overlapping ends of forward and reverse reads. - Recent update simplifies the code: single _parse library used by both parse and parse2, - a number of functions that reduce repetitive code, e.g. push_pair function, - dosctrings and documented structure of _parse library. - Both parse and parse2 have the options to report 5' or 3' ends; to flip alignments according to chromosome coordinate. - Both parse and parse2 have the pysam backend - Improvements of the tests for parse and parse2 - Documentation includes description of various --report-orientation and --report-position cases.
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- new module called by `pairtools header` - submodules: - generate : Generate the header - set-columns : Add the columns to the .pairs/pairsam file - transfer : Transfer the header from one pairs file to another - validate-columns : Validate the columns of the .pairs/pairsam file - resolves #119 - option remove-columns for `pairtools select`: Remove the columns from .pairs/pairsam file
* --tag-mode parameter: XA and XB modes for original and github versions of bwa * XB number of fields problem resolved * comparison of str to integer in alt scores resolved (did not work before!) * reporting scores of optimal, suboptimal and second suboptimal scores added (controlled by --report-scores) * explanations of the inner working added to the docs
…set; - sphinx doc update (added pysam); - header warning if empty and error if try to add a field to empy one
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* Add summaries * Add functions for duplication tile and complexity * Make dedup stats! Co-authored-by: Aleksandra Galitsyna <agalitzina@gmail.com>
* Filtering stats * Multiple filters; save the filtering expression * distance bins saved, saving and reading yaml works * update test * select is now a separate library used by stats and select CLI * Python engine for stats filtering added * black stats and select * travis fix yaml Co-authored-by: Aleksandra Galitsyna <agalitzina@gmail.com>
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pairtools v1.0.0 roadmap updates: #116