rna_amp_eecseq.Rmd

---
title: "RNA library amplification"
output: html_notebook
params:
  num_samples: 1
---
```{r setup}
library(tidyverse)
```

```{r}
rna_amp <- tribble(~item, ~quantity,
                   "kapa_10x_library_amp_master_mix_ul", 15 * params$num_samples, 
                   "tubes_200ul", 1 * params$num_samples,
                   "tips_100", 2 * params$num_samples)
```

- Assemble each library amplifcation reaction as follows:

|Component|Volume|
|---------|------|
|Purified, adapter-ligated DNA| 10 ul|
|Library Amplification Master Mix| 15 ul|
|--Total Volume--| --25 ul--|

- Mix well by pipetting up and down several times
- Amplify the library using the following thermal cycling protocol:

|Step|Temp|Duration|Cycles|
|----|----|--------|------|
|Initial denaturation|98 °C|45 sec|1|
|Denaturation|98 °C|15 sec|12|
|Annealing-|60 °C|30 sec|12|
|Extension|72 °C|30 sec|12|
|Final Extension|72 °C|5 min|1|
|Hold|10 °C | ∞|1|


- Place the plate/tube on ice and proceed to --Library Amplification Cleanup--