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TapeStation 4200

Last updated by Ryan Snow on 2/1/23. The following protcol was designed and adapted from the Agilent D1000 Protocol. Please refer to the manufacturer's kit guide for additional protocol information.

This protocol is intended for Genomic DNA.

Running the TapeStation

  1. Remove samples and reagents from storage

    • Allow sample to acclimate to RT for 30 minutes

  2. Record sample and reagent record log form(s) attached

    • Ladder (Φ) will always be in position A1
    • Store in lab notebook

  3. Launch TapeStation Controller software

  4. Record sample and reagent information in TapeStation Controller software

    • Ladder (Φ) will always be in position A1

  5. Flick ScreenTape(s) to remove any bubbles if necessary

  6. Load TapeStation deck with consumables

    • ScreenTape(s)
      • First ScreenTape will be placed into the ScreenTape nest
      • Additional ScreenTape(s) will be placed in the ScreenTape rack leaving room for the first ScreenTape
    • Tips

  7. Vortex and spin samples

  8. Label tube strip(s) and/or 96-well plates

    • Ensure good documentation practices to track sample position

  9. Prepare TapeStation Ladder in position A1 (3 uL Genomic Sample Buffer:1 uL Genomic Ladder)

    • For 1 ScreenTapes; load 3 uL of Genomic Sample Buffer and 1 uL Genomic Ladder
    • For 2 ScreenTapes; load 6 uL of Genomic Sample Buffer and 2 uL Genomic Ladder
    • For 3+ ScreenTapes; load 15 uL of Genomic Sample Buffer and 5 uL Genomic Ladder

  10. Per sample, load 3 uL Genomic Sample Buffer and 1 uL of DNA sample in planned position(s)

    • Apply lid to sample tube strip and/or plate after adding sample

  11. Vortex at 2000 rpm for 1 minute using the IKAMS3 vortexer

    • Located near the TapeStation

  12. Spin down for 1 minute to remove sample and ladder from lid

  13. Load TapeStation instrument

    • Ladder must be in position A1

  14. Remove sample lid before closing and running instrument

    • Verify sample presence
    • Close TapeStation lid

  15. On the TapeStation computer click Start

    • Confirm information in pop-up dialogue box
    • Move on to Data Analysis after run is complete

Data Analysis

  1. Follow instructions in Run Finished dialogue box

    • Label and store any partially used ScreenTapes as such
    • Discard used tips
      • Best practice to replace tips after use
    • Remove samples from TapeStation
    • Store ScreenTape(s) in Used ScreenTapes container

  2. Save and export data using the following naming convention: [Initials/Personal Identifier][Relevant Run Info]-[MMDDYY]

Ex. for Ryan Snow Test Run on Jan 18th, 2023 SnTR-011823

  1. Data should default to saving in the following location: Documents > Agilent > [Run Date] > [File Name]