tris-hcl_stock.Rmd

---
title: "Tris-HCl stock solution"
params:
  final_conc_M: 0.010
  pH: 8.0
  final_vol_l: 0.050
  mol_wt: 121.1
---
```{r setup, include=FALSE}
library(tidyverse)
library(knitr)
```

## Prepare a stock solution of Tris-HCl  
- usually don't need more than 50mL of solution, it is ok to prepare this in a small, clean, beaker, and transfer to a 50mL falcon tube.  
- the molecular weight of Tris-HCl is 121.1g
```{r tris, echo=FALSE}

tris_hcl <- tribble(~item, ~quantity,
                      "tris-hcl_g", params$final_vol_l * params$mol_wt * params$final_conc_M,
                    "ph2o_ml", params$final_vol_l*1000)

kable(tris_hcl)

```
## Adjust pH to `r params$pH` using HCl or NaOH.