config.toml

[caller_settings.dorado]
config = "rna_rp4_130bps_sup"
address = "ipc:///tmp/.guppy/5555"

[mapper_settings.barcode]
n_threads = 4

[[regions]]
name = "Control"
control = true
min_chunks = 1 # minimum number of chunks before a decision can be made
max_chunks = 1 # maximum number of chunks to use in decision-making - after this perform the above_max_chunks action
targets = []   # Genomic targets for this region
single_on = "stop_receiving"        # Action to take if there is one mapping on target.
multi_on = "stop_receiving"         # Action to take if there is more than one mapping, with at least one target.
single_off = "stop_receiving"       # Action to take if there is one mapping and it is off target
multi_off = "stop_receiving"        # Action to take if there are multiple mappings, where all are off target.
no_seq = "stop_receiving"           # Action to take if there is no sequence information
no_map = "stop_receiving"           # Action to take if there is no mapping information
above_max_chunks = "stop_receiving" # Action to take if the number of chunks received is above max_chunks
below_min_chunks = "stop_receiving" # Action to take if the number of chunks received is below min_chunks

[[regions]]
name = "Analysis"
min_chunks = 1
max_chunks = 10
targets = ["yes"]                # This can only be either "yes" or "no"
single_on = "stop_receiving"     # Action to take if the barcodes were detected in the sequence
multi_on = "stop_receiving"      # This will never be trigerred since the mapper returns a yes/no response
single_off = "unblock"           # Action to take when some or all of the barcodes are detected but are malformed, missing, etc.
multi_off = "unblock"            # Again, this will never be trigerred
no_seq = "proceed"               # Read has not been basecalled
no_map = "proceed"               # No barcodes have not been detected yet
above_max_chunks = "unblock"     # Action to take if the number of chunks received is above max_chunks
below_min_chunks = "proceed"     # Action to take if the number of chunks received is below min_chunks