diff --git a/src/test/java/htsjdk/samtools/cram/structure/SubstitutionMatrixTest.java b/src/test/java/htsjdk/samtools/cram/structure/SubstitutionMatrixTest.java
index f82cd3e4cd..74af86b44c 100644
--- a/src/test/java/htsjdk/samtools/cram/structure/SubstitutionMatrixTest.java
+++ b/src/test/java/htsjdk/samtools/cram/structure/SubstitutionMatrixTest.java
@@ -73,7 +73,6 @@ public void testSingleReferenceBaseSubstitution (
         final SubstitutionMatrix substitutionMatrix = getSubstitutionMatrixForFrequencies(refBase, nA, nC, nG, nT, nN);
         Assert.assertEquals(substitutionMatrix.getEncodedMatrix(), expectedMatrixBytes);
 
-        System.out.println(substitutionMatrix);
         // create a "roundTrip" matrix using the expected bytes as input, and cross-validate that the codes match
         final SubstitutionMatrix roundTrippedMatrix = new SubstitutionMatrix(expectedMatrixBytes);
         for (final SubstitutionBase referenceBase : SubstitutionBase.values()) {