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ApplyTransform.Warp.run() alters the TransformsList such that it can't be used again #1866

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mattcai opened this issue Apr 10, 2020 · 1 comment
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bug An issue with an existing feature
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@mattcai
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mattcai commented Apr 10, 2020

Description

Sometimes, starfish users may want to apply a TransformsList to multiple ImageStacks with ApplyTransform.Warp. For example, applying the same transforms to both dapi and primary ImageStacks. The issue is that TransformsList gets modified when used by ApplyTransform.Warp such that the next time it is used by ApplyTransform.Warp it can return erroneous results or throws an error.

A quick workaround is to only use each TransformsList once, and re-LearnTransform if needed.

Steps/Code to Reproduce

transforms bug gist

Expected Results

The transforms printed before and after Warp.run() are the same. No error is thrown on the second Warp.run().

Actual Results

Before:
tile indices: {<Axes.ROUND: 'r'>: 0}
translation: y=0.0, x=0.0, rotation: 0.0, scale: 1.0
tile indices: {<Axes.ROUND: 'r'>: 1}
translation: y=-102.126, x=194.721, rotation: 0.0, scale: 1.0
tile indices: {<Axes.ROUND: 'r'>: 2}
translation: y=-190.79, x=120.938, rotation: 0.0, scale: 1.0

After:
tile indices: {<Axes.ROUND: 'r'>: 0, <Axes.CH: 'c'>: 2, <Axes.ZPLANE: 'z'>: 0}
translation: y=0.0, x=0.0, rotation: 0.0, scale: 1.0
tile indices: {<Axes.ROUND: 'r'>: 1, <Axes.CH: 'c'>: 2, <Axes.ZPLANE: 'z'>: 0}
translation: y=-102.126, x=194.721, rotation: 0.0, scale: 1.0
tile indices: {<Axes.ROUND: 'r'>: 2, <Axes.CH: 'c'>: 2, <Axes.ZPLANE: 'z'>: 0}
translation: y=-190.79, x=120.938, rotation: 0.0, scale: 1.0

KeyError thrown.

@mattcai mattcai added the bug An issue with an existing feature label Apr 10, 2020
@mattcai mattcai changed the title ApplyTransform.Warp.run() alters the TransformsList such that it can't be used again ApplyTransform.Warp.run() alters the TransformsList such that it can't be used again Apr 10, 2020
@neuromusic neuromusic added this to the 0.3.0 milestone Apr 10, 2020
@neuromusic neuromusic modified the milestones: 0.2.1, 0.2.x, 0.2.2 Aug 7, 2020
@davidhbrann
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It looks like this issue is still present, and due to how the Axes of the selector gets updated. I had to make a deepcopy of TransformsList to warp both a dots fiducial image and an image stack.

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