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bioinformatic_python_tutorial

Tutorial for sequence handling using python

Including step by step python scripts to (main.sh):

  • concatenate forward and reverse fastq files
  • extract reads whose barcode start with A or T (for the sack of practice)
  • convert fastq to fasta file
  • delete N's in fasta file
  • format fasta file so that each read takes two lines (header & seq)
  • count number of bases for each read, output a tab-separated values (TSV) file
  • get reverse complement DNA sequences
  • translate nucleotides to proteins

Using BioPython to do the same steps (biopython_tutorial.py).

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Tutorial for sequence handling using python

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