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Sample Requirements

As part of our request form, please include your sample metadata sheet which you can download through the form or here. This allows us to efficiently track your samples through the downstream processes.

Sample Naming

Samples must be only letters, numbers, and underscores. No other characters including spaces are permitted.

The following CAN NOT be found in sample names: S[0-9]+ , R[0-9], fastq, gz, L[0-9]+

No sample names can be repeated. Please check that all sample names are unique (This includes the controls).

Examples of good names:

Bisanz1_Mouse1Group1

Bethany1_Participant42

Susan6_Mouse3Cdiff

Examples of bad names:

Sample1

NegativeControl

S1

Bisanz1_Cdiff+fecal transplant

Controls: Please remember to assign unique names to any controls.

Examples of good control names:

ProjectID_ZymoCom_Plate[0-9]_[Well]

ProjectID_ZymoDNA_Plate[0-9]_[Well]

ProjectID_ExtCon_Plate[0-9]_[Well]

ProjectID_NTC_Plate[0-9]_[Well]

Physical Storage

For DNA extractions on the QiaCube, please obtain a 96-well PowerBead plate from the Collaboratory ahead of time for your samples. Generally 50-200ul (or 50-200mg) sample are good amounts for extraction.

For DNA, please have your samples arrayed in fully skirted 96-well plates according to your metadata sheet. Concentrations of >1ng/ul for microbial DNA will give the best chance for success.

Please have your samples appropriately labeled with your Project ID, name, and date. For a sealed skirted 96-well plate, a label with Project ID, name, and date placed on the side is sufficient. For individual samples, please array labeled samples in order in a 2" cardboard box with a label of Project ID, name, and date.

Sample Shipping

Upon submission of the OHMC request form, you will receive an email from us to coordinate sample hand off and timing.