1.5.0

[1.5.0] - 30/09/2024

Added

• Processing of RM data. (Twist capture + mtDNA)
  • conf/Autorun.config: Add RP profile for processing twist capture results. Identical to TF for now.
  • scripts/prepare_eager_tsv.R: Add RP analysis type for twist capture results
  • scripts/run_Eager.sh: Run for RP TSVs.
  • scripts/cron_daily_prepare.sh: Create RP analysis TSVs daily.
  • scripts/ethical_sample_scrub.sh: Add RP analysis type for ethical sample scrubbing.
  • scripts/clear_work_dirs.sh: Add RP analysis type for work directory clearing.
  • scripts/clear_results.sh: Add RP analysis type for results directory clearing.
• scripts/update_poseidon_packages.sh: Bump version for new release.
• README.md: Updated to list new state of the pipeline.

Fixed

Dependencies

Deprecated

1.4.0

Add processing of RP data.
Bump to nf-core/eager 2.5.0.
Use mapDamage with a read limit of 100k for damage calculations.

1.3.0

[1.3.0] - 12/07/2023

Added

• scripts/ethical_sample_scrub.sh: A script to remove eager input/outputs for samples that were marked as ethically sensitive after the pipelines picked them up.
• scripts/cron_ethical_scrub.sh: A cron-able script to run ethical_sample_scrub.sh daily.
• scripts/clear_work_dirs.sh: A bash script to rm -r the work directories of an individual ID for both SG and TF processing.
• scripts/clear_results.sh: A bash script that deletes the results for an individual while maintaining the nextflow process cache for them.

Fixed

• scripts/cron_daily_prepare.sh: Silenced permission errors due to ethical sample scrubbing.

Dependencies

Deprecated

1.2.0

[1.2.0] - 21/03/2023

Added

• prepare_eager_tsv.R: Added -w/--whitelist option. A whitelist of Pandora Individual IDs can be provided. Only the TSVs of individuals in the whitelist will be updated.

Fixed

• update_poseidon_packages.sh: Library_Names field now includes only unique library names.
• prepare_eager_tsv.R: Camel_Case versions of Pandora Analysis IDs are no longer filtered out.

Dependencies

Deprecated

1.1.3

[1.1.3] - 17/03/2023

Added

Fixed

• Column naming in fill_in_janno.R. Nr_Libs -> Nr_Libraries.
• prepare_eager_tsv.R no longer joins with non-unique iids. Optimised performance and less likely to kill the TSV maker.
• Increased memory given to eager spawner array jobs.

Dependencies

Deprecated

1.1.2

[1.1.2] - 02/01/2023

Added

• Add -a option to run_Eager.sh to create a text file with the run commands for launching Autorun_eager, for submission as a qsub array.
• Add array submission script.
• Add script to create and update poseidon packages from eager output.
  • Includes script to collate eager results and overwrite the janno of a poseidon pacakge.
  • Includes script to update poseidon indFile from janno files.
  • Each poseidon package contains a file named AE_version.txt with the version used for the last package creation/update.
• Added conda environment yaml file autoeager_env.yml

Fixed

• Fixed pull request template.
• Fixed a bug where sequencing runs were considered updated if the Results.txt was updated without changing the data. Now only changes to the output bams constitute an update trigger.
• Fixed a bug where TSV creation would try to match the sequencing ID to the sequencing batch ID, instead of the run ID.

Dependencies

Deprecated

1.1.1

Bugfix where the TSV generation and version file creation would fail for new directories.

1.1.0

What's Changed

• v1.1.0 by @TCLamnidis in #12

Full Changelog: 1.0.0...1.1.0

[1.1.0] - 07/10/2022

Added

• Added bed file for on-target coverage calculation for TF data. In SG this parameter gets overwritten and hence ignored.
• prepare_eager_tsv.R now also creates a version file within the directory of the TSV.
• Added github PR template.

Fixed

• Parameters that require local paths have been moved to their own profile to aid in reproducibility outside of the EVA cluster.

Dependencies

Deprecated

1.0.0

[1.0.0] - 19/09/2022

Added

• Directory structure now includes a subdirectory with the site ID.
• Jobs are now submitted to all.q

Fixed

• Fixed a bug where the bams of additional Autorun pipelines would be pulled for processing than intended.
• The sample names for single stranded libraries now include the suffix _ss in the Sample Name field. Avoids file name collisions and makes merging of genotypes easier and allows end users to pick between dsDNA and ssDNA genotypes for individuals where both are available.
• Library names of single stranded libraries also include the suffix _ss in the Library Name field. This ensures that rows in the MultiQC report are sorted correctly.

Dependencies

• nf-core/eager=2.4.5

Deprecated

Contributors

• @TCLamnidis
• Thanks to @jfy133 for review comments.