1. The *.fa files were the whole chloroplast genomes of Nicotiana genus.
2. The correspondence between species names and accession numbers can be found in Table 1 of the article 'Comparative chloroplast genomes of Nicotiana species (Solanaceae): Insights into the genetic variation, phylogenetic relationship and polyploid speciation'.
3. We also upload the CDS sequences and gff3 annotation files used in this article.
4. If you have any questions, please feel free to contact us via email at 765278332@qq.com. We're here to assist you.