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shinyApp article image
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Bavanaa committed Jul 5, 2024
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Expand Up @@ -111,13 +111,14 @@ statistical significance (P value) versus magnitude of change (fold change).
Here, the cutoffs for log2FC and P value can be adjusted to set thresholds
for statistical significance.

![](images/VolcanoPlot.png){width=70%}
![](images/VolcanoPlot.png)

### Find Markers

Marker features based on louvain/leiden cluster identities or cell metadata can be defined based on results of wilcoxon rank-sum test via [presto](https://www.biorxiv.org/content/10.1101/653253v1) or measures of cluster specificity with genesorteR (Ibrahim, 2020) . Variable numbers of marker genes per cell group can be specified based on adjusted p value and thresholded log fold change.

![](images/marker_gene_plot.png){width=70%}
![](images/marker_gene_plot.png)


### Subset Seurat Input

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