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HEV-GLUE is an open resource supporting comparative genomic analysis of Hepatitis E virus (HEV), developed using the GLUE software framework.
HEV is a major cause of acute viral hepatitis worldwide, particularly in developing regions with poor sanitation. It belongs to the Hepeviridae family and is classified as a positive-sense, single-stranded RNA virus. HEV primarily affects the liver, leading to inflammation and impaired liver function, though in most cases the infection is self-limiting.
GLUE is an open, integrated software toolkit designed for storing and interpreting sequence data. It supports the creation of bespoke projects that incorporate essential data items for comparative genomic analysis, such as sequences, multiple sequence alignments, genome feature annotations, and other associated data. Projects are loaded into the GLUE "engine," forming a relational database that represents the semantic relationships between data items. This foundation supports systematic comparative analyses and the development of sequence-based resources.
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Comprehensive Database: HEV-GLUE integrates genome feature definitions, reference genome sequences, multiple sequence alignments, and standardised metadata for all HEV genotypes and subtypes.
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GLUE Framework Integration: Built on the GLUE software framework, HEV-GLUE offers an extensible platform for efficient, standardized, and reproducible computational genomic analysis of HEV.
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Phylogenetic Structure: Sequence data in HEV-GLUE is organized in a phylogenetically-structured manner, allowing users to explore evolutionary relationships easily.
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Rich Annotations: Annotated reference sequences enable rigorous comparative genomic analysis related to conservation, adaptation, structural context, and genotype-to-phenotype associations.
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Automated Genotyping: HEV-GLUE can perform automated genotyping of HEV sequences (including subgenomic sequences).
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Exploratory and operational: The GLUE framework allows sequence-based resources to be used for exploratory work in a research setting, as well as more operational processes performed in a public health setting.
To begin using HEV-GLUE for comparative genomic analysis, follow these steps:
- Install GLUE: First, install the GLUE software framework, which forms the foundation of HEV-GLUE.
- Install the HEV-Project: Once GLUE is installed, HEV-GLUE can be installed as a prebuilt database, or constructed from scratch via a local project build process. The prebuilt database allows for quick setup, while the local build process allows for more customization.