AmpliconSeq

A set of protocols and pipelines for construction and analysis of 16S rRNA amplicon libraries.

!!!Currently Under Construction!!!

Wet Lab Protocols

Protocol 1: DNA Extraction
Protocol 2: Primary PCR
Protocol 3: Indexing PCR
Protocol 4: Pooling
Protocol 5: Clean up and size selection
Protocol 6: Library Quantification and QC
Protocol 7: Loading MiSeq

Computational Workflow

Protocol 8: QIIME2 Pipeline (2020.11)

Optional Protocols

Protocol O2: Primary PCR and Indexing for Low Biomass Samples
Protocol O4: Ampure XP Cleanup

Other

Design of primers and index here: .

Helpful Resources