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Wrong output of dropTag in SPLiT-seq mode #124

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realzhang opened this issue May 23, 2021 · 1 comment
Open

Wrong output of dropTag in SPLiT-seq mode #124

realzhang opened this issue May 23, 2021 · 1 comment

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@realzhang
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I found dropTag modified the original sequence of fastq read. Some read are wrongly assigned by sequence of others. There could be some error in the order of processing.

@realzhang
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For example,
Original fq: (SPLiT-seq)

@seq1
AAA
@seq2
**TTT**
@seq3
CCC

After tagging:

@seq1_bc3bc2bc1_UMI
AAA
@seq2_bc3bc2bc1_UMI
**CCC**
@seq3_bc3bc2bc1_UMI
CCC

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