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This project aims to dissect the bile acid metabolism by the human gut microbiome.

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msk5737/BileAcids_sFMT_invitro_221211

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This repo is for the BA_sFMT_invitro experiment run on Dec 11, 2022.

This repository contains the .Rmd files used for data analysis for the bile acid quantitation of sFMT invitro experiment.

Experiment details:

37 strains of sFMT and 22 subgroups contains those strains were OD-normalized and incubated in a 96-well plate for 48 hours in BHI CHV media containing the following bile acids: CA-d5 CDCA-d5 DCA LCA GCA TCA

see BAmetadata and samplemetadata for more info

The strains were extracted and the bile acids were quantitated using targeted metabolomics approach using ThermoFisher TSQ triplequad.

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This project aims to dissect the bile acid metabolism by the human gut microbiome.

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