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modupeore authored Feb 28, 2020
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Thank you for your interest in using the Variant Analysis Pipeline.
VAP is a comprehensive workflow for reference mapping and variant detection of genomic and transcriptomic reads using a suite of bioinformatics tools.

_**Article Source:**_

Variant analysis pipeline for accurate detection of genomic variants from transcriptome sequencing data
Adetunji MO, Lamont SJ, Abasht B, Schmidt CJ (2019) Variant analysis pipeline for accurate detection of genomic variants from transcriptome sequencing data. PLOS ONE 14(9): e0216838. https://doi.org/10.1371/journal.pone.0216838


## Bioinformatic tools
Bioinformatic tools are grouped based on sequencing reads
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* BOWTIE2
* BWA


### Transcriptomic Sequencing
* TOPHAT2
* STAR (2-PASS)
* HISAT2


### Variant Calling (for both Genomic/Transcriptomic Sequencing)
* PICARD + GATK HaplotypeCaller
* sort, addreadgroups, markduplicates using **Picard Tools**.
* split cigar reads using **GATK** from Transcriptomic Sequencing reads.
* variant detection using **GATK**.

**N.B.** : parameters of all tools are set to default.
Contact maintainer to make custom changes to the different tools if needed.

### Software used to design the VAP workflow are:

Software | Version
-------- | -------------
TopHat2 | 2.1.1
HiSAT2 | 2.1.0
STAR | 2.5.2b
SAMtools | 1.4.1
Picard tools | 2.13.2
GATK | 3.8
BWA-mem | 0.7.17
BOWTIE2 | 2.3.5.1

*Current pipeline is not compatible with GATK v4*

Contact maintainer to make custom changes to the different tools


## Things to be aware of
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